LOCUS       sequence-487627-        6768 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  Rab effector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6768)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEmerald RPH3A C3
FEATURES             Location/Qualifiers
     source          1..6768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3013..3022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3496..3617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3533..3552)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3587..3606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3624..4079)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3761..3782)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3973..3992
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4106..4210
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4212..4569
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4212..4403
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4235..4255)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4420..4555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4604..5398
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4604..5398
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4658..4677)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5268..5287
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5586..5605)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5630..5677
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6006..6594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6495..6514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1321 atgactgaca ctgtggtgaa ccgatggatg taccctggtg atggccctct gcagtcaaat
     1381 gacaaggaac agctgcaggc aggatggtcc gtccatcctg gagcacagac cgacaggcag
     1441 aggaagcagg aagaactgac agacgaggag aaggagatca tcaacagagt gattgctcgg
     1501 gcagagaaga tggaagccat ggaacaggaa cgcattgggc gcctggtgga ccgtctggag
     1561 accatgagga agaatgtggc tggagatggc gtgaaccgct gcattctgtg tggggaacag
     1621 ctgggtatgc tgggctcggc ctgtgtcgtg tgtgaagact gtaagaagaa tgtctgcacc
     1681 aagtgtgggg ttgagacctc caacaaccgt ccgcatccgg tatggctctg caagatctgc
     1741 cttgagcaga gagaggtctg gaagcgctca ggagcatggt tcttcaaagg tttccccaag
     1801 caggtccttc cacagcccat gcctataaag aagaccaagc cccagcagcc tgctggtgaa
     1861 ccggccaccc aggagcagcc tacacctgag tccaggcatc cagccagggc tccagctcga
     1921 ggtgacatgg aggacaggag gcccccaggg cagaagccag gccctgacct cacctctgct
     1981 cctgggagag gaagccatgg gcctcccacg cgtagggcct ctgaggcacg gatgagtaca
     2041 gccgcccggg attctgaggg ctgggaccat gcccatggtg ggggtactgg agacaccagc
     2101 cgtagcccag caggtttgag gcgagctaac tcagtccagg cagcccgccc tgccccagcc
     2161 ccagtgccaa gcccagcacc tcctcagccg gtgcagccag ggccccctgg gggcagcagg
     2221 gccactcctg ggccaggacg ctttccggag cagagcacag aggctcctcc aagtgaccct
     2281 ggctatccag gggctgtcgc cccagcccga gaggagagga caggacctgc ggggggcttc
     2341 caggcagcgc cgcacactgc agccccctat tcccaggcag cccctgctcg ccagccacca
     2401 cctgcggagg aggaggagga agaagccaat agttatgact ctgatgaagc aaccacactg
     2461 ggtgccctgg aattcagcct tctctatgac caagacaaca gcaacctgca gtgcaccatc
     2521 atcagggcga agggactgaa gcccatggat tccaatggct tggcagatcc ctatgtgaag
     2581 cttcatctgc tgcctggagc cagcaagtcc aacaagcttc gtacaaagac cctgcgcaac
     2641 actcggaacc ctgtgtggaa tgagacactg cagtatcatg gcattacaga ggaggacatg
     2701 cagaggaaga cactaaggat ctccgtgtgt gacgaggaca agtttggcca caacgagttc
     2761 attggtgaga ccaggttctc gctcaagaag ctgaaggcta accagaggaa aaacttcaac
     2821 atctgcctgg agcgggtgat cccgatgaag agagcaggga ccaccgggtc ggcccgtggc
     2881 atggctctct atgaggagga gcaggtagag cggatcggcg atatagagga acggggcaag
     2941 atcctggtgt ccctcatgta cagcacgcag cagggcggcc tcattgtggg aatcatccgc
     3001 tgtgtgcacc tggccgccat ggatgccaac ggctactcag acccctttgt caagctctgg
     3061 ctgaaaccgg acatggggaa gaaagccaag cacaagactc agattaaaaa gaagaccctg
     3121 aatcccgagt ttaacgagga gttcttttat gatatcaaac acagcgacct ggctaaaaag
     3181 tccctggata tctcggtgtg ggactacgac attggcaagt ctaatgatta catcggaggc
     3241 tgccagctgg ggatctcggc caaaggcgag cgcttgaaac attggtatga gtgtttgaag
     3301 aacaaagaca agaagattga gcgctggcac caactgcaga acgagaacca cgtgtccagt
     3361 gattaaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     3421 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3481 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3541 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3601 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     3661 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3721 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3781 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3841 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3901 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3961 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4021 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4081 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4141 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4201 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4261 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4321 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4381 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4441 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4501 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4561 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4621 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4681 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4741 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     4801 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4861 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4921 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4981 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5041 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5101 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5161 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5221 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5281 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5341 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5401 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     5461 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5521 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     5581 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     5641 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     5701 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     5761 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     5821 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     5881 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5941 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6661 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     6721 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
