LOCUS       sequence-487639-        6949 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  for DNA-free gene knockout editing in rice and wheat or other 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Qiu F, Xue C, Liu J, Li B, Gao Q, Liang R, Chen K, Gao C
  TITLE     An efficient mRNA delivery system for genome editing in plants.
  JOURNAL   Plant Biotechnol J. 2025 Apr;23(4):1348-1358. doi: 
            10.1111/pbi.14591. Epub 2025 Feb 10.
  PUBMED    39928528
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14591";
            journalName: "Plant Biotechnol J"; date: "2025-04"; volume: "23"; 
            issue: "4"; pages: "1348-1358"
COMMENT     Sequence Label: pIVT-Cas9-DEN2
FEATURES             Location/Qualifiers
     source          1..6949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             135..155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             180..4280
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             180..4280
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             4193..4201
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4281..4328
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4779..4795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4779..4795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4792..4814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4803..4819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4827..4833,4834..4851,4852..4857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4872..4893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5010..5027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5181..5769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5261..5280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5940..6731,6732..6800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6563..6582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6801..6905)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctaatacga ctcactatag ggagttgtta gtctacgtgg accgacaaag
       61 acagattctt tgagggagct aagctcaacg tagttctaac agttttttaa ttagagagca
      121 gatctctgat ggctccgaag aagaagagga aggttggcat ccacggggtg ccagctgctg
      181 acaagaagta ctcgatcggc ctcgatattg ggactaactc tgttggctgg gccgtgatca
      241 ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt
      301 ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg gctgaggcta
      361 cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc atctgctacc
      421 tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc cacaggctgg
      481 aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc ttcggcaaca
      541 ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc
      601 tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg gcccacatga
      661 ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat agcgatgttg
      721 acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag aaccccatta
      781 atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag tctcggcgcc
      841 tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc gggaatctca
      901 ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc gctgaggacg
      961 ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc ctggcccaga
     1021 tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc
     1081 tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca gcctccatga
     1141 tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtcaggcagc
     1201 agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc tacgctgggt
     1261 acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg attctggaga
     1321 agatggacgg cacggaggag ctgctggtga agctcaatcg cgaggacctc ctgaggaagc
     1381 agcggacatt cgataacggc agcatcccac accagattca tctcggggag ctgcacgcta
     1441 tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag aagatcgaga
     1501 agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc aactcccgct
     1561 tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg
     1621 tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc gacaagaacc
     1681 tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc acagtctaca
     1741 acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct ttcctgtctg
     1801 gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag gtcacggtta
     1861 agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc gagatctctg
     1921 gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg aagatcatta
     1981 aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac attgtgctga
     2041 cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact tacgcccatc
     2101 tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc tgggggaggc
     2161 tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg atcctcgact
     2221 tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac gatgacagcc
     2281 tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac tcgctgcacg
     2341 agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg cagaccgtga
     2401 aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg
     2461 agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc gagaggatga
     2521 agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag cacccggtgg
     2581 agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat ggccgcgata
     2641 tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc gatcatatcg
     2701 ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc aggtcggaca
     2761 agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag atgaagaact
     2821 actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat aacctcacaa
     2881 aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag aggcagctgg
     2941 tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg atgaacacta
     3001 agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc
     3061 tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc aacaattacc
     3121 accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc aagaagtacc
     3181 caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg aggaagatga
     3241 tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc tactctaaca
     3301 ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg aagcgccccc
     3361 tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga
     3421 ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca gaggtccaga
     3481 ctggcgggtt ctctaaggag tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc
     3541 gcaagaagga ctgggatccg aagaagtacg gcgggttcga cagccccact gtggcctact
     3601 cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg
     3661 agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg atcgatttcc
     3721 tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc cccaagtact
     3781 cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctggc gagctgcaga
     3841 aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg gcctcccact
     3901 acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc gtcgagcagc
     3961 acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag cgcgtgatcc
     4021 tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa
     4081 tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc ggcgcccctg
     4141 ctgctttcaa gtacttcgac acaactatcg atcgcaagag gtacacaagc actaaggagg
     4201 tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg cgcatcgacc
     4261 tgtctcagct cgggggcgac aagcggccag cggcgacgaa gaaggcgggg caggcgaaga
     4321 agaagaagtg aactagtgtc atgggtcgtt taagctgccg atgtgcctgc gtcgtctggt
     4381 gccctctctc catatggagg ttgtcaaagt atctgctgtt cgtgtcatga gtcgtgtcag
     4441 tgttggttta ataatggacc ggttgtgttg tgtgtgcgta ctacccagaa ctatgacaaa
     4501 tcatgaataa gtttgatgtt tgaaattaaa gcctgtgctc attatgttct gtctttcagt
     4561 tgtctcctaa tatttgcctg cagaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4681 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gagacctccc tcacaccggt gacggggatc
     4741 gcatgcgata tctcgagatc tagcttggcg taatcatggt catagctgtt tcctgtgtga
     4801 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     4861 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     4921 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     4981 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5041 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5101 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5161 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5221 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5281 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5341 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5401 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5461 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5521 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5581 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     5641 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5701 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5761 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5821 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5881 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5941 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6001 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6061 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6121 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6181 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6241 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6301 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6361 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6421 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6481 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6541 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6601 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6661 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6721 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6781 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6841 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6901 ccgcgcacat ttccccgaaa agtgccacct gccagtgcca agctaattc
//
