LOCUS       sequence-487640-        6921 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  for DNA-free gene knockout editing in rice and wheat or other 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Qiu F, Xue C, Liu J, Li B, Gao Q, Liang R, Chen K, Gao C
  TITLE     An efficient mRNA delivery system for genome editing in plants.
  JOURNAL   Plant Biotechnol J. 2025 Apr;23(4):1348-1358. doi: 
            10.1111/pbi.14591. Epub 2025 Feb 10.
  PUBMED    39928528
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14591";
            journalName: "Plant Biotechnol J"; date: "2025-04"; volume: "23"; 
            issue: "4"; pages: "1348-1358"
COMMENT     Sequence Label: pIVT-Cas9-TMV
FEATURES             Location/Qualifiers
     source          1..6921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    44..99
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             107..127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             152..4252
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             152..4252
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             4165..4173
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4253..4300
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4751..4767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4751..4767)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4764..4786)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4775..4791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4799..4805,4806..4823,4824..4829))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4844..4865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4982..4999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5153..5741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5233..5252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5912..6703,6704..6772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6535..6554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6773..6877)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctaatacga ctcactatag gggtattttt acaacaatta ccaacaacaa
       61 caaacaacaa acaacattac aattactatt tacaattaca atggctccga agaagaagag
      121 gaaggttggc atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgatat
      181 tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa
      241 gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct
      301 cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg
      361 gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc
      421 gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa
      481 gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa
      541 gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct
      601 ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga
      661 gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac
      721 gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat
      781 cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg
      841 cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa
      901 cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata
      961 cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct
     1021 cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga
     1081 gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga
     1141 tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt
     1201 cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga
     1261 gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt
     1321 gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc
     1381 acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc
     1441 tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta
     1501 cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga
     1561 gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt
     1621 catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca
     1681 ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac
     1741 cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct
     1801 cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa
     1861 gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct
     1921 ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga
     1981 gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat
     2041 gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct
     2101 caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg
     2161 ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg
     2221 caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc
     2281 tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc
     2341 agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat
     2401 gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca
     2461 gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct
     2521 ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct
     2581 ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa
     2641 caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc
     2701 cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc
     2761 ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct
     2821 gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct
     2881 ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt
     2941 tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg
     3001 cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca
     3061 gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc
     3121 ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg
     3181 ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa
     3241 ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac
     3301 tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga
     3361 gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca
     3421 agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct
     3481 gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta
     3541 cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa
     3601 gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg
     3661 ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa
     3721 gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa
     3781 gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa
     3841 gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga
     3901 caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga
     3961 gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct
     4021 ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca
     4081 tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat
     4141 cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc
     4201 gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc
     4261 agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag tgaactagtg tcatgggtcg
     4321 tttaagctgc cgatgtgcct gcgtcgtctg gtgccctctc tccatatgga ggttgtcaaa
     4381 gtatctgctg ttcgtgtcat gagtcgtgtc agtgttggtt taataatgga ccggttgtgt
     4441 tgtgtgtgcg tactacccag aactatgaca aatcatgaat aagtttgatg tttgaaatta
     4501 aagcctgtgc tcattatgtt ctgtctttca gttgtctcct aatatttgcc tgcagaaaaa
     4561 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4681 aagagacctc cctcacaccg gtgacgggga tcgcatgcga tatctcgaga tctagcttgg
     4741 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4801 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4861 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4921 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4981 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5041 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5101 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5161 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5221 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5281 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5341 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5401 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5461 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5521 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5581 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5641 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5701 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5761 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5821 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5881 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5941 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6001 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6061 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6121 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6181 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6241 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6301 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6361 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6421 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6481 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6541 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6601 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6661 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6721 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6781 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6841 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6901 ctgccagtgc caagctaatt c
//
