LOCUS       sequence-487646-       13541 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  For microglia differentiation from iPSC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Drager NM, Sattler SM, Huang CT, Teter OM, Leng K, Hashemi SH, Hong 
            J, Aviles G, Clelland CD, Zhan L, Udeochu JC, Kodama L, Singleton 
            AB, Nalls MA, Ichida J, Ward ME, Faghri F, Gan L, Kampmann M
  TITLE     A CRISPRi/a platform in human iPSC-derived microglia uncovers 
            regulators of disease states.
  JOURNAL   Nat Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 
            10.1038/s41593-022-01131-4.
  PUBMED    35953545
REFERENCE   2  (bases 1 to 13541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2022 Aug 11. pii: 10.1038/s41593-022-01131-4. doi: 
            10.1038/s41593-022-01131-4."
COMMENT     Sequence Label: CYBL-SPI1-CEBPB-IRF5
FEATURES             Location/Qualifiers
     source          1..13541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    856..874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    892..910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    928..946
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    964..982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1000..1018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1036..1054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1072..1090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1135..1159
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1242..1251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2067..2120
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3183..3236
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    4750..4871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4787..4806)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4841..4860
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4920..4936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4920..4936)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4933..4955)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4944..4960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4968..4974,4975..4992,4993..4998))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5013..5034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5151..5168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5460..5479
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5478..5533
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5579..5598
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5689..6435)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6467..6486)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6494..7510
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6548..6570)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        7790..8169
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8175..8399
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8388..8405
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    8421..8454
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8730..8749)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8999..9017
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      9159..9168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9206..9474
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9487..9698)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    9734..9958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(9963..10763)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10074..10093)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10684..10703
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(10758..10811)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10835..10860
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    10861..10894
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(11752..11820,11821..12612)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11970..11989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12783..13371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13272..13291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagcccaga gcagggcctt agggaagcgg gacggctctg ttggagagtc cagtattgaa
       61 ttagtgatag atacacagaa aaaaagcaaa ttgaataggt gaatggataa acaaactgtg
      121 gaacatccag acaatgaaat attcaccaat aaaaagaaat gagctatcaa gccacattgg
      181 ctatgccctt cacatttaag gaggaacctt aaatgcatat tgccaaattt taaaagctag
      241 tctgaaaatg ctacagactg tatgattcca attatatgac attttagaaa aggcaaaact
      301 atagagatca gtggttgcca gcggctcatg gaggcaggat gaattggtgg aacacaggga
      361 tgctttcagg gtaatgaaaa tatcttgtgt gatcctgtaa taatggatac gtggcataat
      421 gcatttgtta aaacctgtaa actagccagg tatagtggct tttttgcacc tatagtctca
      481 gctacttggt aggctgagac aggaagatca cttgagccca ggacttcaag accagcctgg
      541 gtaatatagt aagaccccat ctctattttt taaagaaatt aagaaagaaa agaaacccat
      601 caactgtata acacaaagaa tcgtaatgta aactacagtc ctgagtaatg aaacattttt
      661 aaagaaataa ataaattcaa ggcaattatt aacttcagga aaaacagtaa agtaaacata
      721 agtatacaaa tcaaacatgg ctcagttgtg aaaatattta caaggtcact ttaagtaaca
      781 ttgaatgttg atataatccc aaaatatatt tatgttggaa ggatgaggag atatctccac
      841 cccacagtgg tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
      901 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      961 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
     1021 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
     1081 gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
     1141 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
     1201 tccgtaccac ttcctaccct cgtaaactta aggttaatta agccaccatg gaaggattcc
     1261 ccctggtgcc ccctccctcc gaagatctcg tgccctacga caccgatctg taccagagac
     1321 agacacatga gtattacccc tacctgtcca gcgacggaga gtcccacagc gaccactatt
     1381 gggattttca cccccaccat gtgcactccg agttcgagag ctttgctgag aacaacttca
     1441 ccgagctcca gtccgtgcag cctcctcaac tccagcagct ctatagacac atggagctgg
     1501 agcagatgca tgtgctggat acccccatgg tgcctcccca ccctagcctg ggacaccaag
     1561 tctcctacct gcccaggatg tgcctccagt acccttccct gtcccccgct cagcccagca
     1621 gcgatgagga agaaggcgaa aggcagtccc ctcctctgga ggtgtccgac ggagaggctg
     1681 atggcctcga gcctggaccc ggactgctgc ccggcgagac cggcagcaaa aagaagatta
     1741 ggctgtacca gttcctcctg gatctgctca gatccggcga catgaaggac agcatttggt
     1801 gggtcgacaa ggacaagggc acctttcagt tctccagcaa gcacaaagag gctctggctc
     1861 acaggtgggg catccagaag ggcaacagga agaagatgac ctatcagaag atggccaggg
     1921 ccctcagaaa ctacggcaag accggagagg tgaaaaaagt caagaagaag ctcacatacc
     1981 aattctccgg cgaggtgctg ggaagaggag gactggctga gaggagacac cctcctcact
     2041 ccaggatgca tagcaggggc agcggcgagg gaaggggttc tctgctcacc tgtggagatg
     2101 tcgaggagaa ccccggcccc atgcagaggc tggtggcttg ggaccctgcc tgcctccctc
     2161 tccctcctcc ccctcctgct ttcaagagca tggaggtcgc caacttttac tacgaggccg
     2221 attgtctggc cgccgcttat ggcggaaaag ctgcccccgc cgcccccccc gccgccagac
     2281 ccggccccag gccccccgct ggcgaactgg gaagcatcgg cgaccacgag agggccatcg
     2341 actttagccc ctacctggag cctctcggcg ccccccaasc ccccgcccct gccacagctm
     2401 ccgacacatt cgaggccgcc ccccctgccc ccgcycccgc ccccgcctcc agcggccagc
     2461 accatgactt tctgtccgac ctgttctccg atgactacgg cggcaaaaac tgcaagaaac
     2521 ccgccgagta cggctatgtg tccctgggca ggctgggagc cgctaaagga gccctgcacc
     2581 ctggctgttt tgcccctctg cmcccccccc cccccccccc cccccccccc gccgagctca
     2641 aagctgagcc tggcttcgag cccgccgatt gcaagagaaa agaggaggct ggcgcccctg
     2701 gaggaggcgc cggcatggct gctggattcc cctatgctct gagggcctac ctgggctacc
     2761 aagccgtccc tagcggaagc agcggctccc tgtccacatc ctcctcctcc agccctcctg
     2821 gaacaccttc ccccgccgat gctaaagccc cccccacagc ctgttacgcc ggcgccgctc
     2881 ccgccccttc ccaagtgaaa agcaaggcca agaagaccgt ggacaagcac tccgacgagt
     2941 ataaaatcag aagggaaaga aacaacatcg ccgtcagaaa gtccagggat aaggccaaga
     3001 tgaggaacct cgagacacag cacaaggtgc tcgagctcac cgccgagaac gaaagactgc
     3061 aaaagaaagt cgagcagctc tccagggaac tgtccaccct cagaaacctg ttcaaacagc
     3121 tgcccgagcc cctcctggcc agcagcggcc actgtggaag catgcattcc agaggttctg
     3181 gcgaaggaag gggcagcctg ctgacatgcg gcgacgtgga agaaaatccc ggccccatga
     3241 atcagagcat tcccgtggcc cctacccccc ctagaagagt cagactgaaa ccttggctcg
     3301 tcgcccaggt gaatagctgt cagtaccctg gcctgcaatg ggtgaacgga gagaagaagc
     3361 tcttttgcat cccctggaga cacgctacca ggcacggacc ttcccaggat ggcgacaaca
     3421 ccattttcaa ggcctgggcc aaggagaccg gaaaatacac cgagggcgtc gacgaagccg
     3481 accctgccaa gtggaaagcc aacctcagat gtgccctcaa caagagcagg gatttcaggc
     3541 tgatttacga cggccccagg gacatgcctc ctcaacctta caaaatctat gaggtgtgca
     3601 gcaatggccc tgcccccaca gacagccagc ctcccgagga ctattccttc ggagccggag
     3661 aagaggaaga agaagaagag gagctccaga ggatgctccc cagcctgagc ctcaccgaag
     3721 acgtgaagtg gccccctaca ctccagcctc ccacactgag gccccccaca ctccaacccc
     3781 ctacactgca gccccccgtg gtgctgggcc cccccgcccc cgaccccagc cctctggctc
     3841 ctcctcccgg caatcctgcc ggctttaggg aactgctgag cgaagtcctg gagcccggcc
     3901 ccctgcctgc tagcctgccc cctgctggag agcagctgct gcctgatctc ctcattagcc
     3961 cccacatgct gcccctcacc gatctggaga tcaagttcca gtacagaggc aggcccccca
     4021 gagccctgac catctccaac cctcacggct gtaggctctt ctactcccag ctggaggcca
     4081 cacaagagca ggtggaactg ttcggcccca tcagcctgga acaggtgagg ttcccctccc
     4141 ccgaggatat cccctccgac aagcagaggt tttacaccaa tcagctcctg gatgtgctgg
     4201 acagaggcct gatcctgcag ctgcagggcc aggatctcta cgctatcagg ctctgccagt
     4261 gcaaggtgtt ttggagcgga ccttgtgcta gcgcccacga ctcctgtccc aaccccattc
     4321 aaagagaggt gaagaccaaa ctcttcagcc tcgagcactt cctcaatgag ctcatcctgt
     4381 tccagaaagg ccaaaccaat accccccccc ctttcgagat cttcttctgc ttcggagagg
     4441 agtggcctga caggaagccc agagaaaaaa agctcattac agtgcaggtc gtgcccgtcg
     4501 ctgccagact cctcctggag atgtttagcg gcgagctcag ctggagcgcc gacagcatca
     4561 ggctgcagat cagcaacccc gacctcaaag ataggatggt ggagcagttt aaagagctcc
     4621 accacatctg gcaatcccag cagagactgc agcctgtcgc tcaggctccc cccggcgccg
     4681 gcctgggcgt gggccaaggc ccctggccta tgcaccctgc tggaatgcag tgaatcgatc
     4741 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4801 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4861 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     4921 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4981 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5041 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5101 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5161 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggacggc
     5221 cgcacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa acaaaatata
     5281 aaaaaaatct aacctcaagt caaggctttt ctatggaata aggaatggac agcagggggc
     5341 tgtttcatat actgatgacc tctttatagc caacctttgt tcatggcagc cagcatatgg
     5401 gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg atttgccctc
     5461 ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc aatgaaaata
     5521 aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc cccataattt
     5581 ttggcagagg gaaaaagatc tcagtggtat ttgtgagcca gggcattggc cacaccagtc
     5641 accaccttct gataggcagc ctgcacctga ggagtgaatt cacgcgtttt agcctggcag
     5701 catatcgaga tcgaagtcat cgagagcgtc agcagggagc atatccagat caaaatcatc
     5761 cagggcatcg gcgggcagca tgtccaggtc gaagtcgtcc agggcgtcgg tgggtccgcc
     5821 gctctcgcac ttcagctgct tctcgaggcc gcagatgatc agttccaggc cgaacaggaa
     5881 ggcgggctcg gcgccctgtc tgtcgaacag ctcgatggcc tgcttcagca gggggggcat
     5941 gctgtcggtg gtgggtgtct ctctttcctc tttggcgacc tggtgttcct gttcttccag
     6001 cacgcagccc agggtgaagt ggcccacggc gctcagggcg tacagggcgt tttccaggct
     6061 gaagccctgc tggcacagga aggccagctg gttttccagt gtctcgtact gcttctcggt
     6121 gggtctggtg cccaggtgca ctttggcgcc gtcccggtgg ctcagcaggg cgcatctgta
     6181 gctcttggcg ttgttccgca ggaagtcctg ccagctctcg ccttccagag ggcagctgtg
     6241 ggtgtggtgc cggtccagca tctcgatggg cagggcgtcc agcagggccc gcttgttctt
     6301 cacgtgccag tacagggtgg gctgttccac gcccagtttc tgggccagct tccgggtggt
     6361 caggccctcg atgcccacgc cgttcagcag ttccagggcg ctgttgatca ctttgctctt
     6421 gtccagccgg ctcatgtcga cattctttgc caaaatgatg agacagcaca ataaccagca
     6481 cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca gaggctctag
     6541 agccgccggt cacacgccag aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc
     6601 cgtccccccg cggacagccc cgaggctgga gagggagaag gggacggcgg cgcggcgacg
     6661 cacgaaggcc ctccccgccc atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc
     6721 cgctagaggg ggtgcggcgg cgcctcccag atttcggctc cgcacagatt tgggacaaag
     6781 gaagtccctg cgccctctcg cacgattacc ataaaaggca atggctgcgg ctcgccgcgc
     6841 ctcgacagcc gccggcgctc cgggggccgc cgcgccccyc ccccgagccc yccccggccc
     6901 grggcggccc cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga
     6961 gccccgcgcc acgccccgta cggagccccg cacccgaagc cgggccgtgc tcagcaactc
     7021 ggggaggggg gtgcaggggg ggttgcagcc cgaccgacgc gcccacaccc cctgctcacc
     7081 cccccacgca cacaccccgc acgcagcctt tgttcccctc gcagcccccc ccgcaccgcg
     7141 gggcaccgcc cccggccgcg ctcccctcgc gcacactgcg gagcgcacaa agccccgcgc
     7201 cgcgcccgca gcgctcacag ccgccgggca gcgcggagcc gcacgcggcg ctccccacgc
     7261 acacacacac gcacgcaccc cccgagccgc tccccccgca caaagggccc tcccggagcc
     7321 cctcaaggct ttcacgcagc cacagaaaag aaacaagccg tcattaaacc aagcgctaat
     7381 tacagcccgg aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc
     7441 agagccgggg cgggcggcgc gaggcggcgg cggagcgggg cacggggcga aggcagcgcg
     7501 cagcgactcc cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg
     7561 ccataaaagg aaactttcgg agcgcgccgc tctgattggc tgccgccscm ccycyccccc
     7621 cccccccccc cccccccccc cccccccccc ccccccccgg cgcgcgcccc cccccccccc
     7681 ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt gggggtgggg
     7741 agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc atggtaatag
     7801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     7861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     7921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     7981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     8041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     8101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     8161 atcaatgtcg agacccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     8221 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     8281 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     8341 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
     8401 cgaggctgat cagcgggttt ataacttcgt ataatgtatg ctatacgaag ttatggtacc
     8461 ttactcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat
     8521 ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg
     8581 cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc
     8641 gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat
     8701 ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
     8761 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
     8821 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
     8881 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
     8941 ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt
     9001 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
     9061 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc
     9121 cttgatgatg gccatgttat cctcctcgcc cttgctcacc atggtggcga ccggtagcgc
     9181 tagcgtaggc gccggtcaca gcttggatct gtaacggcgc agaacagaaa acgaaacaaa
     9241 gacgtagagt tgagcaagca gggtcaggca aagcgtggag agccggctga gtctaggtag
     9301 gctccaaggg agcgccggac aaaggcccgg tctcgacctg agctttaaac ttacctagac
     9361 ggcggacgca gttcaggagg caccacaggc gggaggcggc agaacgcgac tcaaccggcg
     9421 tggatggcgg cctcaggtag ggcggcgggc gcgtgaagga gagatgcgag cccctcgaag
     9481 cttcagctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc
     9541 acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact
     9601 ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc
     9661 caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac cggagcgatc
     9721 gcagatcatg catccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     9781 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     9841 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     9901 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacaggt
     9961 ttttagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
    10021 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
    10081 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
    10141 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
    10201 acgacgagat cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc
    10261 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
    10321 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
    10381 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
    10441 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
    10501 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
    10561 cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
    10621 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
    10681 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
    10741 ccatcttgtt caatcctagg gccgggattc tcctccacgt caccgcatgt tagaagactt
    10801 cctctgccct ctccgctgcc agatctctcg aggccctgtg ggaggaagag aagaggtcag
    10861 ataacttcgt ataatgtatg ctatacgaag ttatgcggcc gctgggagaa gggtaagaga
    10921 gctcaatcct catcttacat agaaggaagt cagtagataa tatcttacat ttaaaagtca
    10981 agaaataaca gtctgaccat actatctaga aatatggaag ttaacagtaa ggaagttaaa
    11041 acagttgaaa gtagtttcgt ctagaaagaa agacttgggg aagaacaaag aaggctaatt
    11101 ttcattatga gctttattgt aacagaaaag agaccactaa ctcagctagg ttttcccaat
    11161 tcctcaacac tttggggcac agtgtttagt cagagctgtg atcactctct gagcacatgg
    11221 attgtatctt aattttcatc tcattgaaaa tatgtaccat ttgtccaagt ggaaactggg
    11281 ctgaagcatg actgtgacca ccaccaaaga aatggctaga ctcgctaagg tctgctgggg
    11341 tgacccgtga ttgtgttgtg cttgatcttg ttgagcatgg ctcttttaag tcaccatacc
    11401 tgcccctgcc aatgcaccaa acaccaactt aaagggactt tcagcttttg taagtcagtc
    11461 tcagccttac ttctgaacca tttctttgac ttctttcatg actctgatcc tgtcccagtt
    11521 ttaagcctcc cagccaccag tcatgctgtt ttgctctgct atttacattc ttctctacct
    11581 actttggact tgatggtatg cctactatat tagttattta ttgctgtgta acaaaccact
    11641 ctgaaacata gtggcttaca accacaacca ttttatttta ttatgaaact gtgggtcaga
    11701 atgtatattg gcccattggc atgattctcc tgctattgaa aaaggaagag tatgagtatt
    11761 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    11821 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    11881 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    11941 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    12001 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    12061 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    12121 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    12181 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    12241 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    12301 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    12361 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    12421 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    12481 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    12541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    12601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    12661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    12721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    12781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    12841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    12901 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    12961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    13021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    13081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    13141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    13201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    13261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    13321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    13381 aacgcggcct ttttacggtt cctggcgaat tcgcccttag agcagagcca ggaacccctg
    13441 tagggaaggg gcaggagagc caggggcatg agatggtgga cgaggaaggg ggacagggaa
    13501 gcctgagcgc ctctcctggg cttgccaagg actcaaaccc a
//
