LOCUS       sequence-487696-       14650 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Wang Q, Yang L, Alexander C, Temple S
  TITLE     The Niche Factor Syndecan-1 Regulates the Maintenance and 
            Proliferation of Neural Progenitor Cells during Mammalian Cortical 
            Development.
  JOURNAL   PLoS One. 2012;7(8):e42883. Epub 2012 Aug 24.
  PUBMED    22936997
REFERENCE   2  (bases 1 to 14650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(8)"; pages: "e42883. Epub 2012 Aug 24"
COMMENT     Sequence Label: FUGW-H1-Scrambled control shRNA
FEATURES             Location/Qualifiers
     source          1..14650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1067..1083
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1067..1083
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12809..12825)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12810..12826)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(12850..12869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12851..12869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12876..12893)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12876..12892)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12885..12907)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13034..13489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     13331..13352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13515..13619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13620..13688,13689..14480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13838..13857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg
     1081 atcccccggg ctgcaggaat tcatgtgctc aggcaatttg ttctggcctt cgatgatcat
     1141 ggaaagtatc tcatccttaa aaatctgatg gggacattgc agcacggcgg actaatgggc
     1201 tacctcggag ggatgtacaa ggaccttgtt gacaaggcgc tagggcaatc tggtcccatg
     1261 ccggaagttt atagcggaaa gctcttccgc gagatgttgc tgctctgcgt ctgtgtgctg
     1321 ccccatgacg tgaaatccga tcttcttctg cactccgatc gcctgtgtca tgctctgaat
     1381 atcctacgct actttgcggc aagagacaag aatgatgtga ctggcttctg gcaggctctg
     1441 ccggaaattg aaaaggaatt gctggaccca ctgggaacag cgcttaactt ttcgatggcc
     1501 cattacaagg cgtacaagga gcgcgtcgaa aagggtcaga gtgcttcgga tgatgaacta
     1561 atgcagcgcc aactcaacat gctcgccgtc aacataagca atagcggaat ggctggcggc
     1621 gacgcggaga gcaagctgcc cgacattgga cggcaggaga agctcgatgt gctggccagt
     1681 tccattacca gcttggaaac gctacaatcc ttatatctgc gcgccagaga gatcatcgaa
     1741 cagtccgctc gcctgcgacg ggttgccaat ccatcagatg cttagcttgt actttgtcca
     1801 caatgaaagc gaaatgataa cgtaaactgt gtgtaaacag aaccgaaaac gaaatcgaaa
     1861 cttaaactga tctatgtggt ctgtgggttc ttggctgggg cgataattaa ccgactaatt
     1921 gagccagttg tggtgtgcta actaactggc cagcgatgcg ggctttgcat tgcgatctgc
     1981 gcactgaaat gtagttgcac acaaataggt acacataggt gtgcgtactt aacacctaag
     2041 ctggcagcgt ttttctaatt tttatagcca ttttgaatat gtttttgtat ttcaaatata
     2101 tgaattagct aggcaataaa acaaaactcg ctttttcaaa ccttaaatgg tgttactaca
     2161 aaggctcgcc ttcttgcaga tgcgactagc tcccgtctgg ctccactgca gccgagacca
     2221 ttgacatttt taatagcaag gagttacgga aaggatgaga agacagccaa tctagaagac
     2281 tttagagccc gcgaggagca gcgcgaaagg gagcgggatg cggccgatca agctgccgaa
     2341 aaggcatcac aaaaagaagc aggcggaggt ggtggactgg gtgcaaaagg atcagagtct
     2401 agaggcccag aagatgacgc caagcaagcc aaggtagatg ccataagggc aaagatcctg
     2461 gacgctgcat tgcagcatgt gccccagcag ggctggaccc gccaggccat cattctgggt
     2521 gccgaggaaa gtggctatcc tagcgtggtg cacggcatgt ttcccgaggg tggcttcgcg
     2581 ctggtctccc acttcaacgg caagtgcaac gcccagctgg tggagagcct gcagcaaaag
     2641 acagatggtg gcaagcagga ggtggaggat cctctagatt ttctggtaca agcagtgcgc
     2701 cagcgtctgg agatggttac tccctacaag acgcattggc cccaggcgat ggccctatta
     2761 gcccagcccc agcatgcgcc cacagctctg gcccaggtgc tcaccctggt ggatgacatc
     2821 tgctactact ccggagatcg atctgtggat gtgagtaacg tatagtctcg tatgtacaat
     2881 gcccgactta atcaatgagc ttcctctagt tcggttggta cacgagacgt gtgggtctgg
     2941 cgaccatcat gaagatgacc gagttgtact tcctgcagga caagtcccca ggacatgcgc
     3001 agacctggga gttcctcaag agccgcatgg acgaggctgt gcaactccaa atgatgctat
     3061 cccaaacaga gggcatgacg cacacgttcc aacgctcctt taactcggcg ttcatcacgg
     3121 tgagtttcca ggcatgaatc gcacagtgat ccactaatct ttcatttcta ttgtaggcgc
     3181 gtaatattct cggtctgggc tacaatcgta actagcacgg aagtgaaacc atgtcgggat
     3241 ccgatgcctt gagtgccaca cgcagtccaa agattaataa ttttagtgag aacggttctg
     3301 ctagccctta aagatctaca cctatactta ccttagatcc atatgtatat atgtgcatat
     3361 aagtacatcc tattcaacac ccaatgacag ttttaagctt atttgtattt tcggggaagt
     3421 tgattttaaa tattttgtta cgacatattc tcgatttttc ttactttgct gatattcgta
     3481 gcccaatact gcaaataaag ggggaaaacg ggaatgtgtc cataattttt aaagtttgtt
     3541 tccttattag tgtttcctat taacgttgcc aattttctga atagttggct ctgtccaaac
     3601 gctgactaat caagcgcatg accttcgtgc cctcaaactt gcagttgaaa cgcttaagtg
     3661 attttgcata gatacagctc acggaaattt aaggtttact taaggcttag gtaaatataa
     3721 taaaggttaa acaagctgac gtgctttttt tcgtttcttt acttcgttta agttgaatca
     3781 tagctttgtt tcgtcattca tagaagatac gataggaata agtccacctg tttttccagc
     3841 tcaaccaacg gatcggacaa ctgctccaat aaaaacatct tggttaagct gccatatcca
     3901 ttggttgact gcgttgagtt gcctaaagat ttcctgtgta ctgctgttaa gatcataatg
     3961 ctgggtactg ccatggtagg attgcgtaga tctcctcagt tggtcatcca gatttttata
     4021 gtaatggacc cgcaatccga ccattcgtca ttaatcgatg tgtgtctgcg tggcggagga
     4081 actccagatg gagttcttct gggcgtcaaa gggttcatag aaggttcctt tggtttggtt
     4141 gaagtggctt tgctgtgata tcttattgta agttcttgga agttggactt aaaatcccag
     4201 tactgtctgc tggttccttg aaattcgatt tttttggggt attttgtact gcttgggtta
     4261 aattcgtttt tgatgttgga atgcattttt cacttttact tcttttaggc ggtgggtgtt
     4321 tacccatcat aggcaaggac tttggactga aattcaagat gtttttcaag tggctcggtc
     4381 cataagggga aactcctttg ttggctatgt gcgctaatga tatcaaaact ggactctttg
     4441 gttccgtcac ttcggagtag agatcagata tacttctcac cataaaggga actgttttct
     4501 tcttgtactt tccgtataaa ttattgattt ccagctcgct aagggcgctc aggagctttg
     4561 gaatggacac aacatcgctg tctccaaaga gatctcttaa caatcgtacc agttcgtctt
     4621 gctctcccaa taggagcaat acatctccaa agtcctcgcc gttgccacac cacttgtact
     4681 tgcgattcag cgctctcagc cgaaggatgt agtcctgcat catgtggagt agctttccca
     4741 gagtttcggg agccatggga tcggagaggt aaccattgct atcgaaatgt gcgctggcgg
     4801 agaacaaatc ctgatcggga aacccactta gtggcacctt gtatctcctt cgttttctgg
     4861 atatcatcat cacttttcta tagttggcat acaacaggcg aagcaaaata taatgagctg
     4921 cctctggaga tcgacataat ctttttgcca aggcaaatat gacccgtcga ttgaggtcca
     4981 gccagcgcgg tggcaaggtg tcgcagggat cgggggttgg ctcccgatcc ggacctctta
     5041 tgaaaggatt ctcggcgagg ggccgactgc ccagtggaca gcagctggag atggtgcgca
     5101 ccgggcagtg tggacagctg tagggaatcg gtggacatgg tgtccggcgg aggcgacagc
     5161 agtggatgca catcgatcga ggtggccaga agtagaattg aaacactgta aacaagccag
     5221 cttggaccga ctttcaattt ggaattttca aaaatgatgc tgattggggt ccaaataaac
     5281 gaccttcttt cgaaacctaa gccgcaagga ggcacaaaca ttttgaaatg gtcggtttct
     5341 atgagacata aagggcactg ctagaagttt actctataaa tatcttggtt tttcttataa
     5401 aaaagaaatt aaatttttct taggctagct ttaatgttta ttgctatgca aggttatctg
     5461 gcgcatagca ttgtctgaat tggaactcga atttagaatg tattgcactc gttcattata
     5521 atcttgaagc aatgactatt aattcaaagg ttctaggtat agctcttcct cgcatggcac
     5581 ttgctccact ttactaaatt gtaaaaggta aaaaagtaac gtatacaatt gtctttttaa
     5641 agattactta cctgtttaga ccgtcacatg actttaattt tgactcggtt gcagcttgca
     5701 gttgtttcag cctttctgga acatcgttag tggccaaaat catgccacca agtctgtgtt
     5761 taagggcgga aaatgttggc ctgtgtgatg gcacggagct ccagcagctt tccatcaggg
     5821 aaaacctgag ttaagcaaat gtgatcaaca attatgtact tcgctaagca ctgataccca
     5881 catttcttgc gtacatccct ccggtcgctt catccgatga ccttgtcgca gtagctgcaa
     5941 gagatcactg ggagacaccg acggatatgg cattccaccg agagtggtga tctcatagag
     6001 cagcacacca aaggaccaac taagagggta agatatcagc cacaaaatcg cttcggactt
     6061 aacaaaaata tatacttaca catcgctctg actggtgtac acctggtggg tgagggactc
     6121 cagcgcgagc cacttgatgg gcagcttgcc acttcctccg gactttcggt acacgttctc
     6181 atgatacaca tctcgactca gccttaaaaa aaaaaaacac aatataacta atcattgaac
     6241 tttcagagat acccacccaa aatctgctat cttgatgctg cgatctacgg agattagaac
     6301 attccgggca gccagatccc tatgcactac tttgttttgg gccagaaatt cctaaaatgg
     6361 caaatgatac atgctacact ttttaattac atttagggat agagtagatg taggctacca
     6421 ttcccaccgc cacctgttgg gcgatgtcta gcaaatctgc atatttcagt ggtattcggg
     6481 gcttggtatc cgatgagtcg aggcaatcaa agtattcctt gttctcaaag attcgcttct
     6541 tgttgtccga tttgaaatcc tgcttcgagt ctttcttggg ctgcggcttc agcttccgtt
     6601 ttggctgatc ctttggagct tcagcggttt tgggaatgta actcccaccg atgttttcaa
     6661 tgtcctccag gccaaagccc ttgttgtcgg ctgcattggt tattcgagtg agggtgtagg
     6721 tctcacatcg acttgagtgt gtctgatccg attcactctc ctcttccaca gtggacagca
     6781 tcgagttgtt gatatcctct atgcgatcat ggatggaatt tctagggagt cggttaaacc
     6841 gtcggttgtc cacgttctgt tcaaggttct tcttaagtcc aattgcattt tgctcctgcc
     6901 tgaacttcca ctcctcacta agtaatacat tttttactat acatcacttg ttttccaata
     6961 tgtataaaaa taacttaccg taaaaagttc tgcaggcttc caaggctgca gtattcaatt
     7021 agcaacatca tctggttgct aaagcgagtg gagtatccca cgatacccac aatatttgga
     7081 tgcttgccca cggccttgag catctgaatt tcgcacttga acgcatatac gtcctcgtcg
     7141 tttggttcat ctgtgagaac aaaaattcca tgaattattc aaggacttca ataccaaatg
     7201 gtacaacctt tcagcaactt gacggccact tggcgtttct tgtaaactcc acgtcgcacc
     7261 aagccaaagg ctccttcgcc gaggacatcc tgaaggagca ccgagtgtgg ctccacctcc
     7321 agctcgtcca ttacttccag actctcgttg gccgagaggg tgaccagcag gccactgctg
     7381 tccatcaggg agagatgaaa ttcactggcc ttcgcgtcct tagcgtccat ttgcagcgcc
     7441 tgaacctccg aacgatttcg tctgcagaac gtcagggagc acagcatcaa aatgcagcat
     7501 attggcacga caataaagag taccaacttg accatgttgc cctctgttca atggatggaa
     7561 atggatgtct aaatatttaa tttccttcag cgtacgtacc gctcagcaac acacctcgag
     7621 gaaccttgtc caacgtcaaa ccggatacgt tttttccgcc tgccgactgg gccaccaaat
     7681 gtacttcgaa atgggaaccc agtaccgtga tcttgggtac atagaagtgg ctcctgtttt
     7741 ggtcaagtgt atagtttaac tccgtacctc ctttgaatag atcaaagatg tgaagtgtat
     7801 agttatccgg caggtatctg ggacgcgccc aggtgatgtt cagggccaaa atatttggca
     7861 gatactgctt ctgggtcaca gtaagattct ctggcttatg gggtgctatg aaagggtgga
     7921 acaatattat tattattaat attattatta tataggactt tcgaggaacc tacggcagag
     7981 tgtgtagtta tagggatacc aatccaagca gcttggaaca gcgattggca gccactgcag
     8041 atcgctctcc agtcgattca ttatgttcac cgttcttacg ccaacgtgat actgtttgtc
     8101 aaactccaag ttgtccaccg tgtgcctgta cagctttttg cgctgcagta aaaattagct
     8161 atgctctagc attgcagggg cgtcatggca tactcacatc ccggaactgc acctccagtg
     8221 gctcgtccat attcacgctg ttggttgaat acgacaccat gtcgaaatag cagttgctct
     8281 ctggaatatg aaagtagggt tcgtagatcg ttgattgatt aaactgatgg agctacttac
     8341 ccgccgatgg cttccactcg atttcggcag caatgtgatg caggtcgtct ggttggggga
     8401 caaaccgcag cagattcacc gctcccattt tgctgggctg atagcctcgg atgagggtgg
     8461 cgaaggtctg gtcctttgct atcaaggagt agctgccatc tccgtgcatc gccagggcgg
     8521 tgatgttgtg ggtagagttg gactccaatc ccggcaggat gagaaagttg tcatcagact
     8581 gcaagcgaat aagtcaatga aaaagagcga tccacttgta aaacgaaaca ttaggtcttg
     8641 ttgactacat agtacatctt tctctagaaa ttcatcaaat aaaatgatat tttaaagtaa
     8701 atagtaatga gtttacatct aatatctgac caggtaaagc gtggtttctt tgttgtcgtc
     8761 cttgacagca tccacccgga ttatgtaggt ggcatttgga gccggctcgg ttcccctgct
     8821 gtgctgcacc caatctattt ggaaaacgat ttccgactcc gtgcggcaga tcatgttcat
     8881 ctggtagttt tcctgcactt tgcggagagc cccgggaaat gttccattaa tcctgcagtc
     8941 ctttaaacag cttcggaaat cctgtcgaga aaagaagcat ccacttttgg ttatggagtg
     9001 ttccaacatt ctaagtgggg taaacatacc atctggccct tttccagaca attctgggtg
     9061 caagcggtga cgttgagaag cgtcttatcg tgagagattt tgtccattag caacatttca
     9121 ccttgattgc ttcccacgaa aaaccagaag ataaatgcgt acttcgcata gaaaataagc
     9181 attttccttg ttctgctttt gaaacccttc tttagttctt cgttgtgtgt cggtgtgtgt
     9241 gtgttggaat gaaatgtgta tttgtttaaa accgacttaa agtttattgc acttttgcca
     9301 ccacgcactt tgatacaaca ctcaataaca cacacagcaa attcgaatac ttagtttcta
     9361 gattccaact ttcgagatca ataccgccgt ccttctctga ttatcttgct gaactgagaa
     9421 agaactgaga aactgaggcc aaaaatgcga tggtaaaaaa gcgacgaaga aggaggaatg
     9481 atgagaagcg gcggactcgt tgaatatatt ttgcgctatt ttgttttgat ttgtttttca
     9541 cctcttcact gcatcgtatt gtacttgatc gtatggatgg gattggacta ggggtcattt
     9601 gaaatgcaaa aatttggatc gctactccgt catagtagcg ccagggcttg ggataagcat
     9661 agaacattga cttcagataa ctcttcgcaa ttttcatttg tgataccacc aacaacgttt
     9721 gtatttcaat cttctatgat ctgttgcgcc aaaagagctt gtattgcagc aaaccgataa
     9781 atcagaatgc agtgctggtc gcttccatcc ctagaaacca tgttgggcgc cgatattcga
     9841 aattaagcaa aaaacaacta tgctaatttc gcccacattg tagcttttgc gtgatcacac
     9901 tttccctgat tccatttcaa ttggaacaga gctgacaata caggtatttc cttatagcga
     9961 cttgcaaatg cacatgacct cagttgcctc tgattcaggt tattcgtata gaacgctcga
    10021 ttacattggt gactcaattg ttgcatcgca ttcgagtact cgaaacactc acatgggaaa
    10081 ctcgagcatt gctctctgac ttttcagtgc cactcgcaag tttagttaaa tatagtacgc
    10141 tcgcgtcgtc ggttctgctg ctcaaacgcg ataacgatcc attacgtgtg taaacattaa
    10201 taataaattt ctatgggagc gtctagttta ttaaagctct acaagtttaa aataattagc
    10261 ctgtgatact aggccaaagc ggatcggatc aaaagcaata cgagtaactc ccagttagtt
    10321 ttatcaccat tcaattaaag tgaggaggtt gtcgtgcaac aacagtttcg ttctctttct
    10381 aaaggcattc aaaccagttg caattgaaat ttcaaaacaa taattatata tatgtgatca
    10441 caagtatcgg acaaacgtta aagccggctg ttgttggcaa tgataagatc atatcatata
    10501 atatcatatc ggcattctag ccagacgtcc atatagagca agtcattatc ttgattgctc
    10561 gaatttttaa acaccccaag cgaatagcca ttaaatcgaa ggtatttttt taacaactct
    10621 aaccaatagc cacgcactcg cgccaccgac ggatactgat aaggataaga aatggtaact
    10681 aaacattgaa catttctatt tttcagtcgc cggaggaccg tgctaccaag cagttcaact
    10741 ttccgagttc gagagctctg acatcatgaa aatcgagtgg gctcccaaag gagttcccat
    10801 ggaacggcgt cgccagacgt ttgccatggc cttcctaatc ctatccttta tgatactctc
    10861 ctttggatcc tatttcttcg ttgctgccgt gctggtaaat acatcagtgc ttcaatattt
    10921 tcaaaagtaa agcattccct tccagttcta tggaagcctt ttgtggcgca ccattatggt
    10981 catctatttg gtctacgtct acgcgaatca caagagaacc cactccatta tggatggcaa
    11041 tgggtaggcg tcatgaaatc cctataagaa cacttaaagc taaaataatc cattttagct
    11101 ggaaaattaa ccgcaacaat tggctgtttc ggcattatcg ggactacttt cccgtacagc
    11161 tggtcaagac cgcagagctg ccgccgaata agaactacat tttggccagt tttccgcacg
    11221 gaattttggg gtaagcttgt tgactgatag tcaatcttat cgatggttgc ttaggtgacg
    11281 aaacatttgt ataccttctt acgcacatat tgtaaccctg ataggactgg catttccatt
    11341 aatatgggtc tagacatctc caagtggctg caactgttcc cacaggtgag gcccaaggtg
    11401 gccactttgg atcaaaactt cctgacgccc atcgtgcgtg gtctcctgag atcttggggc
    11461 ctggtgtcgg tttccaagga ggccttagta tatctgctaa ccaaatcaaa cgatcccaag
    11521 cacaaggaca atcgtgatgg gttcacctcc aacgcggtgg ctattctggt gggtggtgcc
    11581 caggaggcac tagactcgca ccctggcaaa tatatattga cactgaagaa ccgcaaggga
    11641 ttcgttaaaa tggccattag aacagggtag gttgaaatcg gatacaacta aaagttagtt
    11701 ttaattgtat ccggtcttca ttttaggtct tcgattgttc ccacgttttc ctttggcgag
    11761 gtggacattc tggatcaggt ggccaatcca ccgaactctc gggttcgtcg ctttcaagac
    11821 tttgtaaaga ggataacggg gatatctccg ctgattcccg ttggccgggg catcttcaac
    11881 tactcctttg gctttctgcc caaccgtcga cgcattgtcc aagttggtga gtcctcagct
    11941 catttaaatg tcgcatagtt gtaagattca aataaactta aaaaaatttc agttggcgct
    12001 cccatcgacg tggttcagag cgatcaacca gacgcagcct atgtggataa gatacacaaa
    12061 caggttattg acgacctgga gaagatgttc gccaagtaca aggatcagta cataccgaac
    12121 tccaagcagg acaagctgat tatacactag tctagaagtt attgtctaaa caatgtgatc
    12181 aaatgagtgc tttataagca tttaatataa atattacgaa ttttatcagg caaataaaat
    12241 tgccatacac gctttttaaa acgacgggta gtgatcgaaa ctttgaataa gcagctatcg
    12301 ctattgatag caattattta tcgataagac ggcaaacagt gttgatcaac gatcagtaga
    12361 agttggcgtt caatttaaaa tgaataacta tgatttatta attgatatat agggaaaaaa
    12421 cataacaatg agcgcagaat ataccaaatg tagtaataac agcattatca tttatttcta
    12481 ataatccctg ttcaaccagc tttattgatt tttaaactac agccttcaat taagaagcaa
    12541 ttatttcatc atacaagcat acaacttaat ccgttgtatg tttttgtaat ttcgttgtat
    12601 gtagtataaa agtaatacag cttatcactt aactgactta taagtcttaa gaaatacgac
    12661 ccacagcata aaatgcttat tgaatcatag tgctcttgaa agtgggtaat ctcctaggaa
    12721 tcgccgcata cctttgcacc cagacggcga ttgcatgccg agtttataat cttcatcact
    12781 ccaatcgcga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
    12841 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
    12901 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    12961 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    13021 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    13081 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    13141 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    13201 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    13261 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    13321 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    13381 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    13441 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    13501 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    13561 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    13621 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    13681 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    13741 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    13801 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    13861 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    13921 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    13981 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    14041 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    14101 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    14161 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    14221 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    14281 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    14341 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    14401 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    14461 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    14521 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    14581 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    14641 aaggatcttc
//
