LOCUS       sequence-487702-        6340 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Distribution and localization of human TBC1D10B in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6340)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple TBC1D10B
FEATURES             Location/Qualifiers
     source          1..6340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3068..3189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3105..3124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3159..3178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3196..3651)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3333..3354)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3545..3564
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3678..3782
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3784..4141
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3784..3975
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3807..3827)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3992..4127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4176..4970
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4176..4970
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4230..4249)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4840..4859
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5158..5177)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5202..5249
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5578..6166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6067..6086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggtc
      781 tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc cgagggcttc
      841 aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgttaa ccaggactcc
      901 tccctgcagg acggcgtgtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccgagga gcggatgtac
     1021 cccgaggacg gcgccctgaa gagcgagatc aagaagaggc tgaagctgaa ggacggcggc
     1081 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacatcg tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tccggactca gatccatgtc tgggaccttg gagtccttgg cggatgatgt gagctccatg
     1321 ggctcagatt cagagataaa cgggctggcc ctgcgcaaga cggacaagta tggcttcctt
     1381 gggggcagcc agtactcggg cagcctagag agctccattc ccgtggacgt ggctcggcag
     1441 cgggagctca gatggctgga catgttcagt aactgggata agtggctgtc acggcgattc
     1501 cagaaggtga agctgcgctg ccggaagggg atcccctcct ctctcagagc caaagcctgg
     1561 cagtacctgt ctaatagcaa ggaacttctg gagcagaacc caggaaagtt tgaggagctg
     1621 gaacgggctc ctggggaccc caagtggctg gatgtgattg agaaggacct gcaccgccag
     1681 ttccctttcc acgagatgtt tgctgctcga ggggggcatg ggcaacagga cctgtaccga
     1741 atcctgaagg cctacaccat ctaccggcct gacgagggtt actgccaggc ccaggccccc
     1801 gtggctgcgg tcctgctcat gcacatgcct gcggagcaag ccttttggtg cctggtgcag
     1861 atctgcgaca agtacctccc aggttactac agtgcagggc tggaggccat tcagctggac
     1921 ggggagatct tttttgcact cctgcgccgg gcctccccgc tggcgcatcg ccacctgcgg
     1981 cggcagcgca ttgaccctgt gctctacatg acggagtggt tcatgtgcat cttcgcccgc
     2041 accctgccct gggcgtcggt gctgcgtgtc tgggacatgt ttttctgtga aggcgttaag
     2101 atcatcttcc gggtggccct ggtcctgctg cgccacacgc tgggctcagt ggagaagctg
     2161 cgctcctgcc aaggcatgta tgagaccatg gagcagctgc gtaacctgcc ccagcagtgc
     2221 atgcaggaag acttcctggt gcatgaggtg accaatctgc cggtgacaga agcactgatt
     2281 gagcgggaga atgcagccca gctcaagaag tggcgggaaa cgcgggggga gctgcagtat
     2341 cggccctcac ggcgactgca tgggtcccgg gccatccacg aggagcgccg gcggcaacag
     2401 ccacccctgg gcccctcctc cagcctcctc agcctccctg gcctcaagag ccgagactcc
     2461 cgggcagctg gaggggcccc gtccccgccg ccccccgtcc gcagagccag tgctgggtct
     2521 gccccagggc ctgtggtcac tgctgaggga ctgcatccat cccttccctc acccactggc
     2581 aatagcaccc ccttgggttc cagcaaggag acccggaagc aggagaagga gcggcagaaa
     2641 caggagaagg agcggcagaa acaggagaag gagcgggaga aggagcggca gaagcaggag
     2701 aaagagcgag agaagcagga aaaggagcga gagaagcagg agaaggagcg gcagaagcag
     2761 gagaagaagg ctcaaggccg gaagctttcg ctgcgtcgaa aggcagatgg gcccccaggc
     2821 ccccatgatg gtggggacag gccctcagcc gaggcccggc aggacgctta cttctgacct
     2881 ctgccctaat cactagtgaa ttcgcggccg cctgcaggtc gacggtaccg cgggcccggg
     2941 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     3001 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     3061 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3181 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3241 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3301 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3361 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3421 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3481 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3541 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3601 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     3661 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3721 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3781 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3841 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3901 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3961 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4021 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4081 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4141 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4201 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4261 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4321 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     4381 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4441 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4501 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4561 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4621 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4681 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     4741 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4801 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4861 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4921 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4981 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     5041 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     5101 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     5161 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5221 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     5281 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     5341 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     5401 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     5461 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5521 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5641 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5701 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5761 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5821 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5881 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5941 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6001 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6061 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6121 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6181 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6241 atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa
     6301 ttacggggtc attagttcat agcccatata tggagttccg
//
