LOCUS       sequence-487708-        5047 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR
  TITLE     Substitution of three amino acids switches receptor specificity of 
            Gq alpha to that of Gi alpha.
  JOURNAL   Nature. 1993 May 20. 363(6426):274-6.
  PUBMED    8387644
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 20. 363(6426):274-6."
COMMENT     Sequence Label: qi5
FEATURES             Location/Qualifiers
     source          1..5047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1779..1797)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1780..1797)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          1881..1946
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2076..2096
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2368..2502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2405..2424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2459..2478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3353..3488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3983..4002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(4115..4573)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4252..4273)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4467..4486
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            complement(join(4696..4715,4716..4800,4801..4875))
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccat gactctggag tccatcatgg catgctgcct gagcgaggag
      721 gccaaggaag cccggaggat caacgacgag atcgagcggc agctgcgcag ggacaagcgc
      781 gacgcccgcc gggagctcaa gctgctgctg ctggggacag gggagagtgg caagtcgacc
      841 ttcatcaagc agatgaggat catccacggg tcgggctact ctgacgaaga caagcgcggc
      901 ttcaccaagc tggtgtatca gaacatcttc acggccatgc aggccatgat cagagcgatg
      961 gacacactca agatcccata caagtatgaa cacaataagg ctcatgcaca attggttcga
     1021 gaggttgatg tggagaaggt gtctgctttt gacgtccccg actacgcggc aataaagagc
     1081 ttgtggaatg atcctggaat ccaggagtgc tacgacagac gacgggaata tcagttatct
     1141 gactctacca aatactatct gaatgacttg gaccgtgtag ccgacccttc ctatctgcct
     1201 acacaacaag acgtgcttag agttcgagtc cccactacag ggatcatcga ataccccttt
     1261 gacttacaaa gtgtcatttt cagaatggtc gatgtagggg gccaaaggtc agagagaaga
     1321 aaatggatac actgctttga aaatgtcacc tccatcatgt ttctagtagc gcttagcgaa
     1381 tatgatcaag ttcttgtgga gtcagacaat gagaaccgca tggaggagag caaagcactc
     1441 tttagaacaa ttatcaccta cccctggttc cagaactcct ctgtgattct gttcttaaac
     1501 aagaaagatc ttctagagga gaaaatcatg tattcccacc tagtcgacta cttcccagaa
     1561 tatgatggac cccagagaga tgcccaggca gctcgagaat tcatcctgaa aatgttcgtg
     1621 gacctgaacc ccgacagtga caaaatcatc tactcccact tcacgtgcgc cacagatacc
     1681 gagaacatcc gcttcgtctt tgcagccgtc aaggacacca tcctgcagct gaaccttaag
     1741 gactgtggcc tcttctgaat gcatctagag ggccctattc tatagtgtca cctaaatgct
     1801 agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     1861 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     1921 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     1981 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     2041 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     2101 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     2161 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     2221 aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     2281 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2341 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2461 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgatcccgc catggtatca
     2521 acgccatatt tctatttaca gtagggacct cttcgttgtg taggtaccgc tgtattccta
     2581 gggaaatagt agaggcacct tgaactgtct gcatcagcca tatagccccc gctgttcgat
     2641 ttacaaacac aggcacagta ctgacaaacc catacacctc ctctgaaata cccatagttg
     2701 ctagggctgt ctccgaactc attacaccct ccaaagtcag agctgtaatt tcgccatcaa
     2761 gggcagcgag ggcttctcca gataaaatag cttctgccga gagtcccgta agggtagaca
     2821 cttcagctaa tccctcgatg aggtctacta gaatagtcag tgcggctccc attttgaaaa
     2881 ttcacttact tgatcagctt cagaagatgg cggagggcct ccaacacagt aattttcctc
     2941 ccgactctta aaatagaaaa tgtcaagtca gttaagcagg aagtgactaa ctgaccgcag
     3001 ctggccgtgc gacatcctct tttaattagt tgctaggcaa ctgccctcca gagggcagtg
     3061 tggttttgca agaggaagca aaaagcctct ccacccaggc ctagaatgtt tccacccaat
     3121 cattactatg acaacagctg ttttttttag tattaagcag aggccggggg cccctggcct
     3181 ccgcttactc tggagaaaaa gaagagaggc attgtagagg cttccagagg caacttgtca
     3241 aaacaggact gcttctattt ctgtcacact gtctggccct gtcacaaggt ccagcacctc
     3301 cataccccct ttaataagca gtttgggaac gggtgcgggt cttactccgc ccatcccgcc
     3361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3421 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3481 tggaggccta ggcttttgca aaaagctaat tcggcgtaat ctgctgcttg caaacaaaaa
     3541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3601 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctgaagccag
     3721 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagagat
     3781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4081 aaaacgccag caacgcaagc tagcttctag ctagaaattg taaacgttaa tattttgtta
     4141 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4201 aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg
     4261 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4321 cagggcgatg gccgcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg
     4381 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     4441 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     4501 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     4561 ctacagggcg cgtactatgg ttgctttgac gagcacgtat aacgtgcttt cctcgttaga
     4621 atcagagcgg gagctaaaca ggaggccgat taaagggatt ttagacagga acggtacgcc
     4681 agctggaccg cggtctttcg gacttttgaa agtgatggtg gtgggggaag gattcgaacc
     4741 ttcgaagtcg atgacggcag atttagagtc tgctcccttt ggccgctcgg gaaccccacc
     4801 acaggtaatg cttttactgg cctgctccct tatcgggaag cggggcgcat catatcaaat
     4861 gacgcgccgc tgtaaagtgt tacgttgaga aagctgctcc ctgcttgtgt gttggaggtc
     4921 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     4981 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     5041 acgcgtt
//
