LOCUS       sequence-487713-       11724 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Fluorescent reporter gene with miRNA-124 target sequence to repress 
            transgene expression in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Deglon Lab
  TITLE     Deglon Lab Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SIN-cPPT-gfa2(B)3-GFP-WPRE-miR124T
FEATURES             Location/Qualifiers
     source          1..11724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      4665..4674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4671..4673,4674..4676,4677..5387)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4671..4673,4674..4676,4677..5387)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4716..4737)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4977..4996)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5324..5345
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5464..5480)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5465..5481)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5494..6082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5547..5567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5965..5976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6286..6519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6544..6562)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6545..6562)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6852..6871)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7085..7104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7204..7226
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7264..7282)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7350..7454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7455..7523,7524..8315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8486..9074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8975..8994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9228..9245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9315..9335)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9320..9649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9500..9635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10783..10848
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10978..10998
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11423..11557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11460..11479)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11514..11533
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatccg atgtacgggc
     2161 cagatatacg cgttgacatt gattattgac tagaccagcc tgggcaacat ggcaagaccc
     2221 tatctctaca aaaaaagtta aaaaatcagc cacgtgtggt gacacacacc tgtagtccca
     2281 gctattcagg aggctgaggt gaggggatca cttaaggctg ggaggttgag gctgcagtga
     2341 gtcgtggttg cgccactgca ctccagcctg ggcaacagtg agaccctgtc tcaaaagaca
     2401 aaaaaaaaaa aaaaaaaaaa aagaacatat cctggtgtgg agtaggggac gctgctctga
     2461 cagaggctcg ggggcctgag ctggctctgt gagctgggga ggaggcagac agccaggcct
     2521 tgtctgcaag cagacctggc agcattgggc tggccgcccc ccagggcctc ctcttcatgc
     2581 ccagtgaatg actcaccttg gcacagacac aatgttcggg gtgggcacag tgcctgcttc
     2641 ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc ccattgagca gggggcttgc
     2701 attgcacccc agcctgacag cctggcatct tgggataaaa gcagcacagc cccctagggg
     2761 ctgcccttgc tgtgtggcgc caccggcggt ggagaacaag gctctattca gcctgtgccc
     2821 aggaaagggg atcaggggat gcccaggcat ggacagtggg tggcaggggg ggagaggagg
     2881 gctgtctgct tcccagaagt ccaaggacac aaatgggtga ggggactggg cagggttctg
     2941 accctgtggg accagagtgg agggcgtaga tggacctgaa gtctccaggg acaacagggc
     3001 ccaggtctca ggctcctagt tgggcccagt ggctccagcg tttccaaacc catccatccc
     3061 cagaggttct tcccatctct ccaggctgat gtgtgggaac tcgaggaaat aaatctccag
     3121 tgggagacgg aggggtggcc agggaaacgg ggcgctgcag gaataaagac gagccagcac
     3181 agccagctca tgtgtaacgg ctttgtggag ctgtcaaggc ctggtctctg ggagagaggc
     3241 acagggaggc cagacaagga aggggtgacc tggagggaca gatccagggg ctaaagtcct
     3301 gataaggcaa gagagtgccg gccccctctt gccctatcag gacctccact gccacataga
     3361 ggccatgatt gacccttaga caaagggctg gtgtccaatc ccagccccca gccccagaac
     3421 tccagggaat gaatgggcag agagcaggaa tgtgggacat ctgtgttcaa gggaaggact
     3481 ccaggagtct gctgggaatg aggcctagta ggaaatgagg tggcccttga gggtacagaa
     3541 caggttcatt cttcgccaaa ttcccagcac cttgcaggca cttacagctg agtgagataa
     3601 tgcctgggtt atgaaatcaa aaagttggaa agcaggtcag aggtcatctg gtacagccct
     3661 tccttccctt tttttttttt ttttttgtga gacaaggtct ctctctgttg cccaggctgg
     3721 agtggcgcaa acacagctca ctgcagcctc aacctactgg gctcaagcaa tcctccagcc
     3781 tcagcctccc aaagtgctgg gattacaagc atgagccacc ccactcagcc ctttccttcc
     3841 tttttaattg atgcataata attgtaagta ttcatcatgg tccaaccaac cctttcttga
     3901 cccaccttcc tagagagagg gtcctcttgc ttcagcggtc agggccccag acccatggtc
     3961 tggctccagg taccacctgc ctcatgcagg agttggcgtg cccaggaagc tctgcctctg
     4021 ggcacagtga cctcagtggg gtgaggggag ctctccccat agctgggctg cggcccaacc
     4081 ccaccccctc aggctatgcc agggggtgtt gccaggggca cccgatcttc ctcttcatgc
     4141 ccagtgaatg actcaccttg gcacagacac aatgttcggg gtgggcacag tgcctgcttc
     4201 ccgccgcacc ccagcccccc tcaaatgcct tccgagaagc ccattgagca ggcccgatct
     4261 tcctcttcat gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac
     4321 agtgcctgct tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag
     4381 caggcccgat cttcctcttc atgcccagtg aatgactcac cttggcacag acacaatgtt
     4441 cggggtgggc acagtgcctg cttcccgccg caccccagcc cccctcaaat gccttccgag
     4501 aagcccattg agcaggcccg ggcatcgcca gtctagccca ctccttcata aagccctcgc
     4561 atcccaggag cgagcagagc cagagcaggt tggagaggag acgcatcacc tccgctgctc
     4621 gcggggatcc aggcctaagc ttacgcgtcc tagcgctacc ggtcgccacc atggtgagca
     4681 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4741 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4801 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4861 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4921 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4981 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5041 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5101 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5161 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5221 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5281 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5341 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     5401 ctcgactagc tagtagctag ctagctagtc gagctcaagc ttcgaattcg atatcaagct
     5461 tatcgatacc gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt
     5521 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5581 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5641 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5701 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5761 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5821 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5881 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5941 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6001 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6061 tccctttggg ccgcctcccc gcatcgggaa ttcgagctcg gtacctaatg gcattcaccg
     6121 cgtgccttaa ttcgaatggc attcaccgcg tgccttaaac gcgttggcat tcaccgcgtg
     6181 ccttaaatgc attggcattc accgcgtgcc ttaaggtacc tttaagacca atgacttaca
     6241 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6301 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg
     6541 tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta
     6601 gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca
     6661 gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg
     6721 agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat
     6781 cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     6841 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     6901 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     6961 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     7021 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc
     7081 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     7141 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     7201 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     7261 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7321 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7681 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8161 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9241 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9301 attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     9361 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9421 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9481 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9541 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9601 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca
     9661 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
     9721 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
     9781 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
     9841 cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta
     9901 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
     9961 gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt
    10021 ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga
    10081 aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt
    10141 tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg
    10201 tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc
    10261 gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct
    10321 ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta
    10381 ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct
    10441 ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg
    10501 tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga
    10561 tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga
    10621 tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg
    10681 gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
    10741 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
    10801 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
    10861 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
    10921 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
    10981 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
    11041 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
    11101 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
    11161 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
    11221 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
    11281 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
    11341 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    11401 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    11461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    11521 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    11581 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    11641 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    11701 aatatgtact acaaacttag tagt
//
