LOCUS       sequence-487900-       11387 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express Hut1p membrane protein cargo, peroxisomal Ynd1p truncation, 
            and Pex22 peroxisomal chauffeur.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11387)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1336
FEATURES             Location/Qualifiers
     source          1..11387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5357..5544
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5412..5436)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5888..6301
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6435..7142
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             7660..8463
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9105..9920)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9105..9920)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9828..9847
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9912..9931
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10145..10733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10634..10653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatggcggga agtacatcca gtttggtgat ctgtgcgatt ggtatatatg
      781 ctactttttt gacgtgggca ttagtacagg agcctttggc tacaagaacc tggccaaact
      841 ccatgggaaa gtttcaattc cctaatgtaa tatccctcat acaagcttct gtggcaatga
      901 tgatgggcta cctgtactta aactggaaga aggtggaata tccgcccaga aaaatgatta
      961 aagatcattg gaaacaattg atgttgatat catttacaca gagtagttca ggcccgctgg
     1021 ctacaacttc tttaaagcac gtggattatc tcacttacat gttagcaaag tcatgcaaga
     1081 tgatccccgt gttgttggtg catttgcttt tgtataggac gcccatagca agccaaaaaa
     1141 aagttgtagc tctcttggta agtctcggtg tcactatttt cactataggt ggcaacgatg
     1201 gtaaaaagtt gaagagatcc tttaatgaga gcggaaatga taacaaatta caagggtttg
     1261 gactactttt ctctagttta ttccttgatg gattgaccaa cgccacccaa gataagctac
     1321 taaaggccaa taaggccaaa gaaaagggaa aacaaacttt gataacaggt gcacacttga
     1381 tgtttacact gaacctcttc gtgatattgt ggaatatttt gtattttatt gtgatcgact
     1441 gcaaacaatg ggataatgcc gtttctgtat tgacgatgga cccacaagtg tggggctatt
     1501 taatgcttta ttcgttctgt ggcgctatgg gacaatgctt tattttctat actctagaac
     1561 aatttggctc tttagttttg atcatgataa cagtcaccag gaaaatggtt tcgatgattt
     1621 tgagtattat tgtcttcggt aagagtgtac gtttccagca atgggtgggg atgtttatag
     1681 tatttggtgg aatcacttgg gaagcactca acaagaagaa ggctaatata ccgaaggcaa
     1741 aatctgcggg ttctgtttct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
     1801 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
     1861 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
     1921 catggccaac cttagtcact actttatctt ggggtgttca atgttttgca agatacccag
     1981 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     2041 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     2101 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     2161 ttttaggtca caaattggaa tacaattatt tctctgacaa tgtttacatc actgctgaca
     2221 aacaaaagaa tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg
     2281 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     2341 cagacaacca ttacttatcc actcaatcta agttatccaa agatccaaac gaaaagaggg
     2401 accacatggt cttgttagaa tttgttactg ctgctggtat taccttgggt atggatgaat
     2461 tgtacaaagg atccgcagag tatgtgcggg ccctgtttga ctttaatggg aatgatgaag
     2521 aagaccttcc ctttaagaaa ggagacatcc tgagaatccg ggataagcct gaagagcagt
     2581 ggtggaatgc agaggacagc gaaggaaaga gggggatgat tcctgtccct tacgtggaga
     2641 agtatggatc tgggagcgga tctggaggca gcatggcgga atatgtacga gctctttttg
     2701 attttaatgg caatgatgag gaggatctcc cttttaaaaa gggcgatatt ctgcgcattc
     2761 gtgataagcc agaggaacaa tggtggaacg ccgaggatag tgaggggaaa cgcggcatga
     2821 ttccggtgcc gtatgtagag aagtactaat ggcgccaatt ggtgcggcaa ttgataataa
     2881 cgaaaatgtc ttttaatgat ctgggtataa tgaggaattt tccgaacgtt tttactttat
     2941 atatatatat acatgtaaca tatattctat acgctataga gaaaggaaat ttttcaatta
     3001 aaaaaaaaat agagaaagag tttcacttct tgattatcgc taacactaat ggttgaagta
     3061 ctgctacttt aattttatgc tgccaaacca gatgtcaaca cagctacaac gtatatatta
     3121 aatttgctct tgttctgtac tttcctaatt cttatgtaaa aagacaagaa tttatgatac
     3181 tatttaataa caaaaaacta cctaagaaaa gcatcatgca gtcgaaattg aaatcgaaaa
     3241 gtaaaacttt aacggaacat gtttgaaatt ctaagaaagc atacatcttc atcccttata
     3301 tatagagtta tgtttgatat tagtagtcat gttgtaatct ctggcctaag tatacgtaac
     3361 gaaaatggta gcacgtcgcg tttatggccc ccaggttaat gtgttctctg aaattcgcat
     3421 cactttgaga aataatggga acaccttacg cgtgagctgt gcccaccgct tcgcctaata
     3481 aagcggtgtt ctcaaaattt ctccccgttt tcaggatcac gagcgccatc tagttctggt
     3541 aaaatcgcgc ttacaagaac aaagaaaaga aacatcgcgt aatgcaacag tgagacactt
     3601 gccgtcatat ataaggtttt ggatcagtaa ccgttatttg agcataacac aggtttttaa
     3661 atatattatt atatatcatg gtatatgtgt aaaatttttt tgctgactgg ttttgtttat
     3721 ttatttagct ttttaaaaat tttactttct tcttgttaat tttttctgat tgctctatac
     3781 tcaaaccaac aacaacttac tctacaacta agatctatgc tcatagaaaa cactaatgat
     3841 cggtttggta tcgtcataga tgcagggtct tcgggttcga gaatccatgt gtttaagtgg
     3901 caggacacgg aatcgcttct tcatgcaaca aaccaggact cacagtccat attacaatca
     3961 gtacctcata ttcatcaaga aaaagactgg acttttaagc tgaatccagg cttgtcgagt
     4021 tttgaaaaaa aacctcaaga tgcgtacaaa tctcacatca agccgttact agattttgct
     4081 aagaatatta tccctgaatc acattggtcg agttgtcccg tttttattca agcaaccgcg
     4141 ggcatgcgcc ttttacctca agacatacaa tcttccattt tggatggttt gtgtcagggg
     4201 ctcaaacacc ctgcagaatt tttggttgag gattgctcag cacaaattca agtcattgat
     4261 ggtgaaaccg aaggtttata tggctggctt ggcttaaact atctatatgg acactttaat
     4321 gactataatc cagaagtctc tgaccatttt acatttgggt ttatggacat gggcggtgcc
     4381 tctactcaga ttgcgtttgc accgcatgat tcaggagaaa tagctagaca tagagatgac
     4441 atcgccacca tcttcttgag gagtgttaac ggagatttgc agaaatggga cgtttttgtt
     4501 agtacgtggt tagggtttgg tgccaatcag gctagaagaa ggtacttggc tcagttgatc
     4561 aatacccttc cagaaaacac aaatgattac gaaaatgatg acttctctac aaggaacttg
     4621 aatgatccat gtatgccgcg aggaagcagc acggattttg aatttaaaga taccatattt
     4681 cacatcgcgg gttctgggaa ttacgaacaa tgtacaaaat ctatttatcc gttactttta
     4741 aagaacatgc cttgtgatga cgagccgtgc ttgtttaatg gtgttcatgc tcctcgaata
     4801 gactttgcca atgacaaatt tataggtact tctgagtact ggtacactgc caacgacgta
     4861 ttcaaacttg ggggcgaata caactttgac aaatttagca aaagcctaag ggagttttgc
     4921 aattccaatt ggacgcaaat attagcgaac agtgataaag gcgtgtataa ttctattccg
     4981 gagaattttt tgaaagatgc atgctttaag ggcaattggg tccttaatat acttcatgaa
     5041 gggtttgata tgcctcgaat agatgtcgac gcagaaaatg tcaatgacag gcccttattt
     5101 caaagtgtgg aaaaagtcga agaacgagag ttatcgtgga cactaggcag aattttactt
     5161 tatgcctcag gtagcatatt ggcaggtaat gatgacttca tggtgggtat tgcgcccagt
     5221 gaaagaagaa ctaagctcac tggtaagaaa ttcatacctg gcaagttact ggagtctgat
     5281 caactacgca agcaaagttc cggatccggt tcattgggaa gaggtagaag atccaaattg
     5341 taataactcg agtggcgcga atttcttatg atttatgatt tttattatta aataagttat
     5401 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     5461 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
     5521 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt
     5581 cgctggatgc acacactggc ttaagatgac aacgccttgc caacagggag ttcttcagag
     5641 acatggaggc tcaaaacgaa attattgaca gcctagacat caatagtcat acaacagaaa
     5701 gcgaccaccc aactttggct gataatagcg tataaacaat gcatactttg tacgttcaaa
     5761 atacaatgca gtagatatat ttatgcatat tacatataat acatatcaca taggaagcaa
     5821 caggcgcgtt ggacttttaa ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc
     5881 cacaagtgat cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc
     5941 agattttctc ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact
     6001 aaatttcccc tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa
     6061 agaaaaaaga caccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat
     6121 cacgtttctt tttcttgaaa attttttttt ttgatttttt tctctttcga tgacctccca
     6181 ttgatattta agttaataaa cggtcatcaa tttctcaagt ttcagtttca tttttcttgt
     6241 tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa
     6301 gttttaatta caaaagatct atgccaccac catcaagaag tagaataaac aaaacaagaa
     6361 cattaggaat agtgggtaca gctatagcag tgttggtcac gtcctactat atatatcaaa
     6421 aggtgacagg ttctgtgtcc aaaggagagg agttaatcaa ggaaaacatg agaatgaaag
     6481 ttgtcatgga gggctccgtt aatggtcacc aattcaagtg tacaggggaa ggtgaaggta
     6541 atccttacat gggtacacaa actatgagaa ttaaagtaat tgaaggcgga ccactaccat
     6601 ttgcatttga cattctggca acgtcattca tgtacggatc acgaactttc atcaagtacc
     6661 ctaaaggtat accagacttt ttcaagcaat cttttccaga gggttttaca tgggaaaggg
     6721 ttacaagata cgaagatggg ggtgtcgtca cagttatgca agatacttca ttagaagatg
     6781 gctgccttgt ctatcatgtg caagtaagag gggtgaattt tccttctaac ggacctgtga
     6841 tgcagaaaaa gaccaaaggt tgggaaccaa atactgaaat gatgtaccca gctgatggag
     6901 gtttgagagg ctacacacac atggcgctta aagttgatgg tggaggtcat ttgtcttgta
     6961 gttttgttac cacttatcgt tctaaaaaga ctgttggcaa tatcaaaatg ccaggaatac
     7021 atgctgtaga ccacagacta gaaagactcg aagagagcga taacgaaatg ttcgttgtac
     7081 agagagagca tgccgtagcc aaatttgctg gcttaggcgg tggtatggat gaattgtata
     7141 agggatccgg gtcaggctcc ggatctggcc ctcccccagc tcttccgcca aaaagacgaa
     7201 ggtaatggca gtgcttttaa ctaagaatta ttagtctttt ctgcttattt tttcatcata
     7261 gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta aaaattgata
     7321 cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg cactggaagg
     7381 ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa aaatgctgag
     7441 cagttacaga gatgttacga accactagtg cactgcagta cacggtttcc ttgaaatttt
     7501 tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg
     7561 gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa
     7621 ctgcacagaa caaaaacgtg caggaaacga agataaatca tgtcgaaagc tacatataag
     7681 gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa
     7741 aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta
     7801 gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat
     7861 ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta
     7921 ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg
     7981 ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca
     8041 ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt
     8101 ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt
     8161 actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac
     8221 atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat
     8281 gacaagggag atgcattggg tcaacagtat agaaccgtgg atgatgtggt ttctacagga
     8341 tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag
     8401 ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac
     8461 taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt
     8521 taattatatc agttattacc cgagtagagc acttgaatcc actgccccgg gaatctcggt
     8581 cgtaatgatt tctataatga cgaaaaaaaa aaaattggaa agaaaaagct tcatggcctt
     8641 tataaaaagg aactatccaa tacctcgcca gaaccaagta acagtatttt acggggcaca
     8701 aatcaagaac aataagacag gactgtaaag atggacgcat tgaactccaa agaacaacaa
     8761 gagttccaaa aagtagtgga acaaaagcaa atgaaggatt tcatgcgttt gtactctaat
     8821 ctggtagaaa gatgtttcac agactgtgtc aatgacttca caacatcaaa gctaaccaat
     8881 aaggaacaaa catgcatcat gaagtgctca gaaaagttct tgaagcatag cgaacgtgta
     8941 gggcagcgtt tccaagaaca aaacgctgcc ttgggacaag gcttgggccg ataaggtgta
     9001 ctggcgtata tatatctaat tatgtatctc tggtgtagcc catttttagc atgtaaatat
     9061 aaagaccgag cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     9121 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     9181 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     9241 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     9301 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     9361 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     9421 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgaggcga
     9481 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     9541 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     9601 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     9661 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     9721 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     9781 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     9841 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt
     9901 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     9961 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
    10021 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc atgaccaaaa
    10081 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10141 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10261 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10741 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10801 ctgcgttatc ccctgattct gtggataacc gtgcggccgc caatgctaaa ttcgagtgaa
    10861 acacaggaag atcagaaaat cctcatttca tccatattaa caataatttc aaatgtttat
    10921 ttgcattatt tgaaactagg caagacaagc aacgaaacgt ttttgaaaat tttgagtatt
    10981 ttcaataaat ttgtagagga ctcagatatt gaaaaaaagc tacagcaatt aatacttgat
    11041 aagaagagta ttgagaaggg caacggttca tcatctcatg gatctgcaca tgaacaaaca
    11101 ccagagtcaa acgacgttga aattgaggct actgcgccaa ttgatgacaa tacagacgat
    11161 gataacaaac cgaagttatc tgatgtagaa aaggattaaa gatgctaaga gatagtgatg
    11221 atatttcata aataatgtaa ttctatatat gttaattacc ttttttgcga ggcatattta
    11281 tggtgaagga taagttttga ccatcaaaga aggttaatgt ggctgtggtt tcagggtcca
    11341 taaagcccac atggataaca ttacccctga attcgcatct agactga
//
