LOCUS       sequence-487903-        9403 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Constitutive expression of a MESA NTEVp chain encoding (N to C): 
            VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard 
            JN
  TITLE     Conversion of natural cytokine receptors into orthogonal synthetic 
            biosensors.
  JOURNAL   Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 
            10.1038/s41589-025-01986-1.
  PUBMED    40846994
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 
            10.1038/s41589-025-01986-1."
COMMENT     Sequence Label: L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in...
FEATURES             Location/Qualifiers
     source          1..9403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        359..1541
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          590..1532
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1489..1509
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1543..1567
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1711..1776
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4483..4707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             4850..5644
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4904..4923)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5514..5533
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5675..5769
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     enhancer        5790..6169
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6170..6373
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6324..6344
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6418..6437
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6418..6436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6456..6465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6717..6726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7206..7430
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7476..7904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7563..7582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7773..7794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7927..8123
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7974..8109
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8036..8055
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8243..8364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8280..8299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8334..8353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8413..8429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8413..8429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8426..8448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8437..8453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8461..8467,8468..8485,8486..8491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8506..8527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8644..8661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8815..9403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8895..8914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag cgatcgaata
      181 ttagatctga gacccaattg acgcgtgagt aattcataca aaaggactcg cccctgcctt
      241 ggggaatccc agggaccgtc gttaaactcc cactaacgta gaacccagag atcgctgcgt
      301 tcccgccccc tcacccgccc gctctcgtca tcactgaggt ggagaagagc atgcgtgagg
      361 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      421 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      481 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      541 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      601 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      661 ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      721 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      781 ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      841 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      901 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      961 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     1021 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     1081 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     1141 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     1201 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1261 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1321 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1381 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1441 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1501 cagtggttca aagttttttt cttccatttc aggtgtcgtg agagctcgtt tagtgaaccg
     1561 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagag aacgggaccg
     1621 atccaaattc tcgaggctag ctgtacagcc accatgcaga gcaaggtgct gctggctgtt
     1681 gccctgtggc tgtgcgtgga aaccagagcc gactacaagg accacgacgg cgattataag
     1741 gatcacgaca tcgattacaa agacgacgac gacaaggcca gcgtgggcct gccttctgtt
     1801 tctctggatc tgcccagact gagcatccag aaggacatcc tgaccatcaa ggccaacacc
     1861 acactccaga tcacctgtcg gggacagcgc gatctggatt ggctgtggcc taacaatcag
     1921 agcggcagcg agcagcgcgt ggaagtgaca gagtgttccg atggcctgtt ctgcaagacc
     1981 ctgacaatcc ccaaagtgat cggcaacgac accggggcct acaagtgctt ctacagagag
     2041 acagacctgg cctccgtgat ctacgtgtac gtgcaggact acagaagccc ctttatcgcc
     2101 agcgtgtccg atcagcacgg cgtggtgtat atcaccgaga acaagaacaa gaccgtggtc
     2161 atcccctgcc tgggcagcat cagcaacctg aatgtgtccc tgtgcgcccg gtatcctgag
     2221 aagagattcg tgcccgacgg caacagaatc agctgggaca gcaagaaggg cttcactatc
     2281 cccagctaca tgatcagcta cgccggcatg gtgttctgcg aggccaagat caacgacgag
     2341 agctaccaga gcatcatgta tatcgtggtg gtcgtgggct accgcatcta cgatgtggtg
     2401 ctgagcccta gccacggcat cgaactgtct gtgggagaga agctggtgct gaactgcacc
     2461 gccagaaccg agctgaatgt gggcatcgac ttcaactggg agtaccccag cagcaagcac
     2521 cagcacaaga aactggtcaa ccgggacctg aaaacccaga gcggctccga gatgaagaag
     2581 ttcctgagca cactgacaat cgacggcgtg accagaagcg accagggcct gtatacttgt
     2641 gccgcctcta gcggcctgat gaccaagaaa aacagcacct tcgtgcgggt ccacgagaag
     2701 ccttttgtgg cctttggcag cggcatggaa agcctggtgg aagccacagt gggcgagaga
     2761 gtcagaatcc ccgctaagta cctgggctac cctccacctg agatcaagtg gtacaagaac
     2821 ggcatccctc tggaaagcaa ccacacaatc aaggccggcc acgtgctgac aatcatggaa
     2881 gtgtccgaga gagacaccgg caattacacc gtgatcctga ctaaccccat cagcaaagaa
     2941 aagcagagcc acgtggtgtc cctggtggtg tatgtgcctc ctcagatcgg cgagaagtcc
     3001 ctgatcagcc ccgtggacag ctaccagtac ggcaccacac agacactgac ctgcaccgtg
     3061 tacgccattc ctccacctca ccacatccac tggtactggc agctggaaga ggaatgcgcc
     3121 aacgagcctt ctcaggccgt gtccgtgaca aacccctatc cttgtgaaga gtggcggagc
     3181 gtggaagatt tccaaggcgg caacaagatc gaagtcaaca agaaccagtt cgccctgatc
     3241 gagggcaaga acaaaacagt gtctaccctc gtgatccagg ccgccaatgt gtctgccctg
     3301 tataagtgcg aggccgtgaa caaagtcggc agaggcgaaa gagtgatcag cttccacgtg
     3361 acaagaggcc ccgagatcac cctccagcca gatatgcagc ctaccgagca agagtccgtc
     3421 agcctgtggt gtaccgccga cagatccacc ttcgagaacc tgacctggta caagctggga
     3481 ccccagcctc tgcctattca cgtgggagaa ctgcccacac ctgtgtgcaa gaacctggac
     3541 accctgtgga aactgaacgc caccatgttc agcaacagca ccaacgacat tctgatcatg
     3601 gaactgaaga acgccagcct ccaggaccag ggcgattatg tgtgtctggc ccaggacaga
     3661 aagaccaaaa agcggcactg tgtcgtgcgg cagctgaccg tgcttgaaag agtggcccca
     3721 accatcaccg gcaacctgga aaatcagacc accagcatcg gcgagtccat cgaggtgtca
     3781 tgtaccgcct ctggcaaccc acctccacag atcatgtggt tcaaggacaa cgagacactg
     3841 gtcgaggaca gcggcatcgt gctgaaggac ggcaatcgga atctgaccat tcggagagtg
     3901 cggaaagagg acgagggact gtatacctgc caggcctgta gcgtgctggg ctgtgctaaa
     3961 gtggaagcct tctttatcat cgagggcgct caagaaaaga ccaacctcga aatcatcatc
     4021 ctcgtgggca cagccgtgat cgccatgttc ttttggctgc tgctcgtgat catcctgcgg
     4081 accgtgaaag gcgccgagag cttgtttaag gggccgcgtg attacaaccc gatatcgagc
     4141 accatttgtc atttgacgaa tgaatctgat gggcacacaa catcgttgta tggtattgga
     4201 tttggtccct tcatcattac aaacaagcac ttgtttagaa gaaataatgg aacactgttg
     4261 gtccaatcac tacatggtgt attcaaggtc aagaacacca cgactttgca acaaagcctc
     4321 attgatggga gggacatgat aattattcgc atgcctaagg atttcccacc atttcctcaa
     4381 aagctgaaat ttagagagcc acaaagggaa gagcgcatat gtcttgtgac aaccaacttc
     4441 caaacttagg cggccgcgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4501 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4561 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4621 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagtc aatagcaggc
     4681 atgctgggga tgcggtgggc tctatgggac gtcggaattg caagttgggc cgccctctgg
     4741 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     4801 gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4861 agatggattg cacgcaggtt ctccggcggc ttgggtggag aggctattcg gctatgactg
     4921 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcagggtcg
     4981 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     5041 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcggctgtgc tcgacgttgt
     5101 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5161 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5221 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5281 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5341 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgt atgcccgacg gcgaggatct
     5401 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5461 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5521 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5581 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5641 ctgagcggga ctctggggtt cgagaattgg cttggactcc tgttgataga tccagtaatg
     5701 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     5761 ggtgagaatc caagcactag ccatgaccag acattgatta ttgactagtt attaatagta
     5821 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     5881 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     5941 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6001 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6061 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6121 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     6181 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     6241 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     6301 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     6361 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
     6421 tacgactcac tatagggagt cccaagctga ctagcgccac catggtttcc aagggcgaag
     6481 aggacaatat ggccagcctg cctgccacac acgagctgca catctttggc agcatcaacg
     6541 gcgtggactt cgacatggtc ggacaaggca ccggcaatcc caacgacggc tacgaggaac
     6601 tgaacctgaa gtccacaaag ggcgacctcc agttcagccc ctggattctg gtgcctcaca
     6661 tcggctacgg cttccaccag tacctgcctt atcctgacgg catgagccct ttccaggccg
     6721 ccatggttga tggctctggc taccaggtcc accggaccat gcagtttgag gatggcgctt
     6781 ccctgaccgt gaactacaga tacacctacg agggcagcca catcaaaggc gaggcccaag
     6841 tgaaaggcac aggcttccct gctgatggcc ccgtgatgac caattctctg acagccgccg
     6901 actggtgcag aagcaagaaa acatacccca acgacaagac catcatcagc accttcaagt
     6961 ggtcctacac caccggcaac ggcaagcggt acagaagcac cgccagaacc acctacacct
     7021 tcgccaaacc tatggccgcc aactacctga agaaccagcc tatgtacgtg ttccgcaaga
     7081 ccgagctgaa gcactccaag acagaactga acttcaaaga gtggcagaaa gccttcaccg
     7141 acgtgatggg catggacgaa ctctacaaat gagcggccaa gtttaaaccc gctgatcagc
     7201 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     7261 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7321 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     7381 ggattgggaa gtcaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     7441 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7681 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7801 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     7921 tgtcagttag gctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8041 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8101 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggaggtt
     8161 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8221 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8341 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8401 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8461 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8521 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8581 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8641 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8701 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caa
//
