LOCUS       sequence-487909-        3111 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Fragmid fragment: (promoter) CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3111)
  TITLE     Fragmid
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AA583
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     enhancer        444..747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        748..951
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     902..922
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(1021..1037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1021..1037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1034..1056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1045..1061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1069..1075,1076..1093,1094..1099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1114..1135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1252..1269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1423..2011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1503..1522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2189..2998)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2912..2931
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2990..3009
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta caccgttaca taacttacgg taaatggccc gcctggctga
      481 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      541 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      601 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      661 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      721 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      781 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      841 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      901 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc taggagcgtc
      961 ttcatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg tgtaatcatg
     1021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaagcc caatctgaat
     2161 aatgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     2221 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     2281 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     2341 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     2401 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     2461 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     2521 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     2581 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     2641 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg
     2701 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     2761 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     2821 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaagc
     2881 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2941 cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa
     3001 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
     3061 tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc a
//
