LOCUS       sequence-487918-        9949 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Constitutive expression of a MESA CTEVp chain encoding (N to C): 
            VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard 
            JN
  TITLE     Conversion of natural cytokine receptors into orthogonal synthetic 
            biosensors.
  JOURNAL   Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 
            10.1038/s41589-025-01986-1.
  PUBMED    40846994
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 
            10.1038/s41589-025-01986-1."
COMMENT     Sequence Label: L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..9949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        359..1541
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          590..1532
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1489..1509
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1543..1567
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1648..1657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1732..1797
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4447..4467
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             4486..4506
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4513..4662
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     polyA_signal    5026..5250
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             5393..6187
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5447..5466)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6057..6076
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6218..6312
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     enhancer        6333..6712
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6713..6916
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6867..6887
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6961..6980
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6961..6979
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6999..7008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7752..7976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8022..8450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8109..8128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8319..8340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8473..8669
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8520..8655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8582..8601
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    8789..8910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8826..8845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8880..8899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8959..8975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8959..8975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8972..8994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8983..8999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9007..9013,9014..9031,9032..9037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9052..9073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9190..9207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9361..9949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9441..9460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag cgatcgaata
      181 ttagatctga gacccaattg acgcgtgagt aattcataca aaaggactcg cccctgcctt
      241 ggggaatccc agggaccgtc gttaaactcc cactaacgta gaacccagag atcgctgcgt
      301 tcccgccccc tcacccgccc gctctcgtca tcactgaggt ggagaagagc atgcgtgagg
      361 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      421 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      481 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      541 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      601 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      661 ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      721 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      781 ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      841 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      901 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      961 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     1021 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     1081 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     1141 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     1201 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1261 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1321 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1381 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1441 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1501 cagtggttca aagttttttt cttccatttc aggtgtcgtg agagctcgtt tagtgaaccg
     1561 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagag aacgggaccg
     1621 atccaaattc tcgaggctag ctgtacagcc accatggtgt cctactggga taccggcgtt
     1681 ctgctgtgtg ccctgctgtc ttgtctgctg ctgaccggat ctagcagcgg cgactacaag
     1741 gaccacgacg gcgattataa ggatcatgac atcgattaca aggacgatga cgacaagagc
     1801 aagctgaagg accccgagct gagcctgaag ggaacccagc atattatgca ggccggccag
     1861 acactgcacc tccagtgtag aggcgaagcc gctcacaaat ggtccctgcc tgagatggtg
     1921 tccaaagaga gcgagcggct gagcatcacc aagagcgcct gtggcagaaa cggcaagcag
     1981 ttctgctcca cactgaccct gaatactgcc caggccaacc acaccggctt ctacagctgc
     2041 aagtatctgg ccgtgcctac cagcaagaag aaagaaaccg agagcgccat ctacatcttc
     2101 atcagcgaca ccggcagacc cttcgtggaa atgtactctg agatccccga gatcatccac
     2161 atgaccgagg gcagagagct ggtcatccct tgcagagtga caagccccaa catcaccgtg
     2221 actctgaaga agttccctct ggacacactg atccccgacg gcaagagaat catctgggac
     2281 agccggaagg gcttcatcat cagcaacgcc acctacaaag agatcggcct gctgacctgt
     2341 gaagccaccg tgaacggcca cctgtataag accaactacc tgacacacag gcagaccaac
     2401 accatcatcg acgtgcagat cagcacccct cggccagtga aactgctgag aggacacacc
     2461 ctggtgctga attgcaccgc caccacacct ctgaacacca gagtgcagat gacctggtct
     2521 taccccgacg agaagaacaa gcgggcttcc gtgcggagaa gaatcgacca gagcaacagc
     2581 cacgccaaca tcttctactc cgtgctgacc atcgacaaga tgcagaacaa ggacaagggc
     2641 ctgtatacct gtagagtgcg gagcggccct agcttcaaga gcgtgaacac ctccgtgcat
     2701 atctacgaca aggccttcat cacagtgaag caccggaagc agcaggtcct ggaaacagtg
     2761 gccggcaaga ggtcctacag actgagcatg aaagtgaagg ctttcccatc tcctgaggtc
     2821 gtgtggctga aggatggact gcctgccaca gagaagtctg ccagatacct gaccagaggc
     2881 tacagcctga tcatcaagga cgtgaccgag gaagatgccg gcaactacac catcctgctg
     2941 tccatcaagc agagcaacgt gttcaagaac ctgaccgcca ctctgatcgt gaacgtgaag
     3001 ccccaaatct atgagaaggc cgtgtccagc tttcccgatc ctgctctgta tcccctgggc
     3061 tccagacaga tcctgacctg taccgcctat ggcatccctc agcctaccat caagtggttc
     3121 tggcacccct gcaaccacaa ccacagcgag gccagatgcg acttctgtag caacaacgag
     3181 gaatccttca tcctggacgc cgacagcaac atgggcaaca gaatcgagag catcacacag
     3241 cgcatggcca tcattgaggg caagaacaag atggcctcaa ctctggtggt ggccgactcc
     3301 agaatcagcg gcatctatat ctgtatcgcc agcaacaaag tgggcaccgt gggcagaaac
     3361 atcagcttct acatcaccga tgtgcccaac ggcttccacg tgaacctgga aaagatgccc
     3421 accgaaggcg aggacctgaa gctgagctgt accgtgaaca agttcctgta ccgggatgtg
     3481 acctggattc tgctgcggac agtgaacaac cggaccatgc actacagcat cagcaagcag
     3541 aagatggcca ttaccaaaga gcacagcatc accctgaatc tgaccatcat gaacgtgtcc
     3601 ctgcaagaca gcggcaccta cgcctgtaga gcccggaatg tgtataccgg cgaggaaatc
     3661 ctgcaaaaga aagagatcac catccgggac caagaggccc cttacctgct gagaaacctg
     3721 agcgatcaca ccgtggccat cagctccagc accacactgg actgtcatgc caatggcgtg
     3781 cccgagcctc agatcacctg gtttaagaac aaccacaaga tccagcaaga gcccggcatc
     3841 attctcggcc ctggaagctc taccctgttc atcgagagag tgaccgaaga ggacgagggc
     3901 gtgtaccact gcaaggccac caatcagaaa ggcagcgtgg aaagcagcgc ctacctgacc
     3961 gttcagggca ccagcgacaa gtccaacctg gaactgatca ctctgacctg cacctgtgtg
     4021 gccgctacac tgttttggct gctgctcacc ctgtttattc ggaagatgaa gcggggcgcc
     4081 aagagcatgt ctagcatggt gtcagacact agttgcacat tcccttcatc tgatggcata
     4141 ttctggaagc attggattca aaccaaggat gggcagtgtg gcagtccatt agtatcaact
     4201 agagatgggt tcattgttgg tatacactca gcatcgaatt tcaccaacac aaacaattat
     4261 ttcacaagcg tgccgaaaaa cttcatggaa ttgaagacaa atcaggaggc gcagcagtgg
     4321 gttagtggtt ggcgattaaa tgctgactca gtattgtggg ggggccataa agttttcatg
     4381 gtgaaacctg aagagccttt tcagccagtt aaggaagcga ctcaactcat gaatcttaag
     4441 ggctcggaga acctgtattt tcagatgggg ggaggcagcg ggggccctaa gaaaaagcgc
     4501 aaagtctccg gagacgctct ggatgacttt gatctggaca tgcttggttc cgatgccttg
     4561 gacgattttg acctcgatat gctgggctcc gacgctctcg acgatttcga cctcgacatg
     4621 ctgggtagcg atgcactgga tgactttgat ctggatatgc tcggatccgg tggtggcggg
     4681 tctggaggag gaggtagtgg tggaggtggc tctggtaccg ctagacccgg cgaaagacct
     4741 ttccagtgcc ggatctgcat gaggaacttc agcaagggcg agagactcgt gcggcacacc
     4801 agaacacaca caggcgagaa gcccttccag tgtagaatct gtatgcgcaa cttcagccgg
     4861 atggacaacc tgagcaccca cctgagaacc cataccgggg agaagccatt tcaatgccgc
     4921 atctgtatga gaaatttttc ccggaaggac gccctgaacc ggcacctgaa aacacacctg
     4981 agaggcagct gagcggccgc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     5041 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5101 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5161 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gtcaatagca
     5221 ggcatgctgg ggatgcggtg ggctctatgg gacgtcggaa ttgcaagttg ggccgccctc
     5281 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
     5341 atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5401 acaagatgga ttgcacgcag gttctccggc ggcttgggtg gagaggctat tcggctatga
     5461 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5521 tcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     5581 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcggctg tgctcgacgt
     5641 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5701 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5761 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5821 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5881 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgtatgcccg acggcgagga
     5941 tctcgtcgtg acccacggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6001 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6061 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6121 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6181 cttctgagcg ggactctggg gttcgagaat tggcttggac tcctgttgat agatccagta
     6241 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     6301 attggtgaga atccaagcac tagccatgac cagacattga ttattgacta gttattaata
     6361 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     6421 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6481 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     6541 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     6601 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     6661 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     6721 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     6781 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     6841 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     6901 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     6961 taatacgact cactataggg agtcccaagc tgactagcgc caccatggtt tccaagggcg
     7021 aagaactgat caaagaaaac atgcacatga agctctacat ggaaggcacc gtggacaacc
     7081 accacttcaa gtgtaccagc gaaggcgagg gcaagcctta cgagggcacc cagaccatga
     7141 gaatcaaggt ggtggaaggc ggccctctgc ctttcgcctt tgatatcctg gccaccagct
     7201 ttctgtacgg cagcaagacc ttcatcaatc acacccaggg catccccgat ttcttcaagc
     7261 agagcttccc cgagggcttc acctgggaga gagtgaccac atacgaggat ggcggcgtgc
     7321 tgacagccac acaggataca agcctccagg acggctgcct gatctacaac gtgaagatcc
     7381 ggggcgtgaa cttcaccagc aacggccccg tgatgcagaa gaaaaccctt ggctgggaag
     7441 ccttcaccga gacactgtat cctgccgatg gcggcctgga aggcagaaac gatatggccc
     7501 tgaagctcgt cggcggctct cacctgattg ccaatgccaa gaccacctac agaagcaaga
     7561 agcccgccaa gaacctgaag atgcccggcg tgtactacgt ggactaccgg ctggaaagaa
     7621 tcaaagaggc caacaacgag acatacgtgg aacagcacga ggtggccgtg gccagatact
     7681 gtgatctgcc ttctaagctg ggccacaagc tgaactgagc ggccaagttt aaacccgctg
     7741 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7801 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7861 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7921 gggggaggat tgggaagtca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7981 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     8041 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     8101 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     8161 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8221 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8281 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8341 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8401 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     8461 aatgtgtgtc agttaggctc aattagtcag caaccatagt cccgccccta actccgccca
     8521 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8581 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8641 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8701 gaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8761 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8821 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8881 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     8941 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9001 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9121 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa
//
