LOCUS       sequence-487923-        7518 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_004, derived from human fusion protein ADIPOR2_FOXJ2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7518)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_004
FEATURES             Location/Qualifiers
     source          1..7518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1051..1761,1762..1764,1765..1767))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1093..1114)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1442..1461
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1701..1722
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1764..1773
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1849..2052)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1878..1898)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2053..2356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2613..2657)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2842..3075
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3435..3552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4044..4169
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4216..4473
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4475..4854
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4885..4989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4990..5058,5059..5850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5208..5227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6021..6609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6510..6529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6763..6780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6848..6982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6872..6891)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6926..6945
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6988..7183)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7002..7137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7242..7263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7278..7283,7284..7301,7302..7308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7316..7332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7321..7343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7340..7356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7340..7356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca cgtgatcagg tcccaatcaa agtcatcggg
      601 aatttcctca ttggctccag gaggcggaac gctggggcga gggaccatgt ggtgtgcagg
      661 gcggttgtaa ccggctgaat cctgggtggg aatgggatac atgggtgatg gtcccggata
      721 caagtgaggt gcttggggat gtccgtatga attaacttga ctcatagcac tctcttgctt
      781 tcccgatgtt aaagcacaag aatccgcgat acgtgcaggc gcgggagggc gatgggtgcc
      841 gccctgaggg ggcacatgtc cggtttgtgt cagaaaatga ttcagagagt cgcacaaatt
      901 agcaatgtga tgctggtcca aggtccaatt cttttgctca gcctgacgaa gggactccat
      961 taactcactg aactgggatt gttcaatact gctccaattc agacggttcc gggaagcggc
     1021 cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat
     1081 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     1141 gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     1201 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     1261 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     1321 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     1381 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     1441 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     1501 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
     1561 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     1621 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     1681 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     1741 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcggtac
     1801 cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag ctctgcttat
     1861 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     1921 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     1981 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     2041 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     2101 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     2161 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     2221 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     2281 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     2341 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     2401 ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg
     2461 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     2521 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     2581 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     2641 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     2701 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     2761 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     2821 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     2881 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     2941 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     3001 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     3061 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     3121 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     3181 ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc catgttctaa
     3241 tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc ataatcccta
     3301 atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg tattactact
     3361 gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt tctgctgtcc
     3421 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
     3481 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
     3541 tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag atgttcagcc
     3601 tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc tactactcct
     3661 tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg ctgtgctttt
     3721 ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     3781 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     3841 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     3901 gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     3961 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     4021 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     4081 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     4141 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     4201 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     4261 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     4321 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     4381 ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct
     4441 cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg actaatacgt
     4501 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     4561 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     4621 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     4681 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     4741 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     4801 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcgaccca
     4861 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4921 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4981 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5041 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5101 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5161 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5221 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5281 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5341 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5401 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5461 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5521 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5581 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5641 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5701 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5761 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5821 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5881 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5941 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6001 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6061 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6841 tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6901 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6961 tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     7021 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     7081 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     7141 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg ctggcacgac
     7201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7321 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg
     7381 caggtcgagg gatctccata agagaagagg gacagctatg actgggagta gtcaggagag
     7441 gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa
     7501 taacacaaaa caaaatat
//
