LOCUS       sequence-487927-        8079 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_008, derived from human fusion protein ARID1B_QKI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8079)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_008
FEATURES             Location/Qualifiers
     source          1..8079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1282..1299)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1303..1320)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1612..2322,2323..2325,2326..2328))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1654..1675)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2003..2022
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2262..2283
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2325..2334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2410..2613)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2439..2459)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2614..2917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3174..3218)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3403..3636
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3996..4113
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4605..4730
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4777..5034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5036..5415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5446..5550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5551..5619,5620..6411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5769..5788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6582..7170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7071..7090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7324..7341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7409..7543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7433..7452)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7487..7506
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7549..7744)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7563..7698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7803..7824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7839..7844,7845..7862,7863..7869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7877..7893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7882..7904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7901..7917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7901..7917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gcccccggca cccgcgccgc ccccaccgcc
      601 accgccaccg ctgccgcctc cgccgccccc cccaccacct ccaccaccac cggcgcccgg
      661 acgactgtaa cccggataat ggttgcattg gctgttcggg taaccctcat ggctattctg
      721 cagcggatcc atgctgcccg gcgcgccggc cgcagccgcg ctagcgctat gcatcatacc
      781 catgcccggg ctctgttggc cgccgtgctg atcaaaacac ggacctgctc tcccacccgg
      841 accgccggaa gctggcgctg ccgagccgta atagttgttg aactctggca cagcagccgg
      901 gccgcccccg ccaccaggga ctgcaaccgg cggctgctgc gtacccagcg cacccagtcc
      961 cggatgttca taacgaccac ccgccggctc gcccattttt ggcggtgcgt cgtcttcatc
     1021 tcccggctta cttagcagag gcggacccgg cggatctgcc tggccacccg ccgcgctgtc
     1081 ctttgcccca ccatgctgcg gctgctccat atccgggccc ggctgcggtg cgccgccacc
     1141 tgcgccacca agagaattgt tgttgctgat tgggtgctgt tgttgctgct gttgttgttg
     1201 ctgctgctgc tgttgttgtt gttgctgttg aaactgattc agttgttgct gcaacgcgtg
     1261 gtggtgatgc aggtgatgcg cgtgatggtg gtggtggtgg gcgtggtggt gatggtggtg
     1321 ttgctgatgc ggaggcgctg ccggagcttc accaacggtc ttcagtttat ggtttggcag
     1381 taaaccggtc tccatagcac tacccggacc agaggatgag gaggaggacg ccgcagccgc
     1441 cgccgcagag gaagaggacg aggaggacaa cgccgctgcg ctaccaccct ccttcaaagc
     1501 tgcttcagag ccaccgctct tagcgctatg cgtgcctgcc gcagcagcgg ctcctgcatt
     1561 gtgtgccatc cgggaagcgg ccgcacttcc cccggagcct ccgcttccgc ccttgtacag
     1621 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     1681 gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg
     1741 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     1801 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     1861 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     1921 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     1981 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     2041 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     2101 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     2161 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     2221 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2281 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg
     2341 tggatcccgg gcccgcggta ccgtcgactg cagaattcgg ttcactaaac gagcccggtt
     2401 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     2461 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     2521 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     2581 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     2641 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     2701 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     2761 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     2821 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2881 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     2941 aactaatgac cccgtaattg attactatta ataactagca cgcgttcgaa gcttgagctc
     3001 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     3061 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     3121 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     3181 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     3241 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     3301 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     3361 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     3421 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     3481 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     3541 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     3601 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     3661 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     3721 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctta tggtgtttta
     3781 ctaaactttt ccatgttcta atcctcatcc tgtctacttg ccacacaatc atcacctgcc
     3841 atctgttttc cataatccct aatgatcttt gcttttcttc ttggcactac ttttatgtca
     3901 ctattatctt gtattactac tgccccttca cctttccaga ggagctttgc tggtcctttc
     3961 caaagtggat ttctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
     4021 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     4081 ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtact
     4141 gctgtcttaa gatgttcagc ctgatctctt acctgtccta taattttctt taattcttta
     4201 ttcatagatt ctactactcc ttgactttgg ggattgtagg gaatttccag gtctgaagat
     4261 cctgctgctt gctgtgcttt tttcttactt ttgttttgct cttcctctat cttgtctaaa
     4321 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     4381 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     4441 atttgtctac agccttctga tgtttctaac aggccagtat taactgcgaa tcgttctagc
     4501 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     4561 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     4621 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     4681 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     4741 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     4801 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     4861 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     4921 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct
     4981 tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctcccatgg
     5041 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     5101 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     5161 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     5221 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     5281 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     5341 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     5401 tcaataatca atgtcgaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5521 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5581 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5641 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5701 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5761 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5821 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5881 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5941 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6001 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6061 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6121 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6181 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6241 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6301 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6361 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6421 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgcttctgtg
     7261 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7321 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7381 gcgcgttggc cgattcatta atgcagcgga tccagacatg ataagataca ttgatgagtt
     7441 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7501 tattgcttta tttgtaacca ttataagctg caataaacaa gttagctttt tgcaaaagcc
     7561 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     7621 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     7681 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     7741 atgcggatcc gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7921 gattacgcca agcttgggct gcaggtcgag ggatctccat aagagaagag ggacagctat
     7981 gactgggagt agtcaggaga ggaggaaaaa tctggctagt aaaacatgta aggaaaattt
     8041 tagggatgtt aaagaaaaaa ataacacaaa acaaaatat
//
