LOCUS       sequence-487942-        7896 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_028, derived from human fusion protein C11orf95_RELA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7896)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_028
FEATURES             Location/Qualifiers
     source          1..7896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1429..2139,2140..2142,2143..2145))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1471..1492)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1820..1839
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2079..2100
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2142..2151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2227..2430)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2256..2276)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2431..2734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2991..3035)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3220..3453
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3813..3930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4422..4547
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4594..4851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4853..5232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5263..5367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5368..5436,5437..6228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5586..5605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6399..6987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6888..6907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7141..7158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7226..7360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7250..7269)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7304..7323
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7366..7561)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7380..7515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7620..7641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7656..7661,7662..7679,7680..7686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7694..7710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7699..7721
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7718..7734
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7718..7734
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gtcgccgctt aataagccgt tgggcaagcc
      601 cggtgcaccc agcggcgctg gagccgggtc cggcggacgc tgcgcgcccg tcaccagacg
      661 ggtgatcgct tccgggtatt ccatcagcat cggttcggtg gtgtgcgggg ccaccggaat
      721 gccctgattt agcagctgtt ggaactcact gttgtccacg cttgccaagt cggtaaacac
      781 cgccgggtcc gttgagttgc ccaacagcgc gccaagatcc tcatcatcaa attgcagctg
      841 cagcagcgcc tccgacagcg taccctcacc cgcctgtgtc ggcttcgggg ccggaggcgc
      901 gaccgcttga ggcggacccg gcgccaggac tggaaccggt gccggcgctt gagccagagc
      961 gcttaccatg gccggagccg gcgccggagc cggggcttgg ggcagaacct gcggtggcgc
     1021 tggcgccaaa gcgctcgctt gtgagatttg accagacggg aacaccatcg tcgggaactc
     1081 atcatagtta atggtggaca aagatgaagt aaacgggtag ggctgaggcg ccggcttcgg
     1141 cacggaagca gagctccgag acggcaccgc aatgcgacgc gggggcggac gtgggtcggt
     1201 cgggccgcta aacgggctct ttttcatgat gcttttaaag gtttcgtacg tacgtttgcg
     1261 cttctcctca atgcggtggc ggtcgtcggt gtccggcaga tattggaact ccatcggttc
     1321 ggacagttca cggtcgcttg ggcgacgcag ctgcatgctc acgcgcaccg gcgcttgcag
     1381 gctcggccgg gaagcggccg cacttccccc ggagcctccg cttccgccct tgtacagctc
     1441 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     1501 cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag
     1561 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     1621 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
     1681 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     1741 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     1801 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     1861 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     1921 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
     1981 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     2041 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     2101 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg
     2161 atcccgggcc cgcggtaccg tcgactgcag aattcggttc actaaacgag cccggttcac
     2221 taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     2281 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     2341 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     2401 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     2461 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     2521 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     2581 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     2641 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     2701 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     2761 taatgacccc gtaattgatt actattaata actagcacgc gttcgaagct tgagctcggt
     2821 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     2881 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     2941 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     3001 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     3061 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     3121 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     3181 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     3241 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     3301 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     3361 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     3421 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     3481 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     3541 tatttatata attcacttct ccaattgtcc ctcatatctc ctccttatgg tgttttacta
     3601 aacttttcca tgttctaatc ctcatcctgt ctacttgcca cacaatcatc acctgccatc
     3661 tgttttccat aatccctaat gatctttgct tttcttcttg gcactacttt tatgtcacta
     3721 ttatcttgta ttactactgc cccttcacct ttccagagga gctttgctgg tcctttccaa
     3781 agtggatttc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt
     3841 tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg
     3901 taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtactgct
     3961 gtcttaagat gttcagcctg atctcttacc tgtcctataa ttttctttaa ttctttattc
     4021 atagattcta ctactccttg actttgggga ttgtagggaa tttccaggtc tgaagatcct
     4081 gctgcttgct gtgctttttt cttacttttg ttttgctctt cctctatctt gtctaaagct
     4141 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     4201 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     4261 tgtctacagc cttctgatgt ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc
     4321 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     4381 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     4441 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     4501 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     4561 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     4621 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     4681 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     4741 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat
     4801 atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct cccatggtaa
     4861 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     4921 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     4981 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     5041 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     5101 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     5161 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     5221 ataatcaatg tcgacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5281 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5341 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5401 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5461 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5521 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5581 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5641 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5701 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5761 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5821 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5881 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5941 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6001 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6061 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6121 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6181 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6241 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6301 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6361 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6421 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6481 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6541 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6601 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6661 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6721 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6781 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6841 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6901 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6961 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7021 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7081 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7141 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7201 cgttggccga ttcattaatg cagcggatcc agacatgata agatacattg atgagtttgg
     7261 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     7321 tgctttattt gtaaccatta taagctgcaa taaacaagtt agctttttgc aaaagcctag
     7381 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     7441 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     7501 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     7561 cggatccgct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7621 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7681 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7741 tacgccaagc ttgggctgca ggtcgaggga tctccataag agaagaggga cagctatgac
     7801 tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg aaaattttag
     7861 ggatgttaaa gaaaaaaata acacaaaaca aaatat
//
