LOCUS       sequence-487951-        7857 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_037, derived from human fusion protein CREBBP_ATG10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7857)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_037
FEATURES             Location/Qualifiers
     source          1..7857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1390..2100,2101..2103,2104..2106))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1432..1453)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1781..1800
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2040..2061
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2103..2112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2188..2391)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2217..2237)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2392..2695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2952..2996)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3181..3414
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3774..3891
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4383..4508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4555..4812
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4814..5193
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5224..5328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5329..5397,5398..6189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5547..5566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6360..6948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6849..6868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7102..7119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7187..7321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7211..7230)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7265..7284
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7327..7522)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7341..7476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7581..7602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7617..7622,7623..7640,7641..7647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7655..7671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7660..7682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7679..7695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7679..7695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ggcagtattc agaccagcat gacctgtcat
      601 ttgaggagat acctgagtta aggtctctgc caggacagag cttgaggctc cttgcatggc
      661 tggtgttgga tatggcatgc ctgccccacg tccgcgacct gcagcaccca atgacccatt
      721 cataacttgg gcttggcctt gagatgcctg attgatcaag ctatgcccgg agttcgagtt
      781 taacagtcca ggatgtgttt ggttgaagtt tgcgttcaca caaatgccgg gaccagtttg
      841 tgacgttgct ggcgaagacg tagccagtcc aacctgcttt tgcgcctgtg gattaagtgc
      901 ttggctcgcc gcgggtgttg ggccggacgt actggctgcc tgctttggca gcgagggagc
      961 actactgtcg ccctgactta aaggactttt tcccattgcg ctaaggctag ccatattcgc
     1021 agaattaggt tgtccttgag cctgcccccc taagccttgt tggacgggcg aggacgcaga
     1081 tacgttccca atgccagggt taattgaaga accactacct ccacgcagta actcggataa
     1141 ctgtttgtgt tttgatgctg cgtcaggaac aaggttaccc gaatttaaaa gccctaactc
     1201 gccgccattt ggaataagct cgtcaggcag atcgttttct aagtcgaaca aagagccaaa
     1261 atcggttgaa tcgtttgccg aaaaaccggg cgaagacaat ttagcgcgtt tcgggtttgg
     1321 cggtccgtca agtaagttct ctgccatccg ggaagcggcc gcacttcccc cggagcctcc
     1381 gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     1441 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct
     1501 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     1561 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     1621 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     1681 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     1741 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     1801 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     1861 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     1921 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     1981 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     2041 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     2101 caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca gaattcggtt
     2161 cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
     2221 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     2281 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     2341 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     2401 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     2461 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     2521 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     2581 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     2641 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     2701 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagcacg
     2761 cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     2821 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     2881 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     2941 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     3001 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     3061 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     3121 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     3181 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     3241 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     3301 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     3361 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     3421 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     3481 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     3541 cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg tctacttgcc
     3601 acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc ttttcttctt
     3661 ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc tttccagagg
     3721 agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac ccgaaaattt
     3781 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     3841 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     3901 aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac ctgtcctata
     3961 attttcttta attctttatt catagattct actactcctt gactttgggg attgtaggga
     4021 atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt gttttgctct
     4081 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     4141 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     4201 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccagtatta
     4261 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     4321 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     4381 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
     4441 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
     4501 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
     4561 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4621 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4681 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4741 aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc
     4801 aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4861 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4921 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4981 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5041 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5101 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     5161 attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt tcggggaaat
     5221 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5281 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5341 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5401 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5461 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5521 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5581 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5641 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5701 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5761 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5821 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5881 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5941 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6001 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6061 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6121 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6181 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6241 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6301 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6481 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7021 ttatcccctg cttctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7081 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7141 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc cagacatgat
     7201 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     7261 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7321 tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     7381 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     7441 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     7501 ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga ctggaaagcg
     7561 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7621 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7681 ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg atctccataa
     7741 gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa
     7801 aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatat
//
