LOCUS       sequence-487952-        8139 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_038, derived from human fusion protein CREBBP_ZNF384.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8139)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_038
FEATURES             Location/Qualifiers
     source          1..8139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1672..2382,2383..2385,2386..2388))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1714..1735)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2063..2082
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2322..2343
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2385..2394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2470..2673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2499..2519)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2674..2977
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3234..3278)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3463..3696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4056..4173
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4665..4790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4837..5094
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5096..5475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5506..5610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5611..5679,5680..6471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5829..5848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6642..7230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7131..7150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7384..7401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7469..7603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7493..7512)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7547..7566
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7609..7804)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7623..7758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7863..7884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7899..7904,7905..7922,7923..7929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7937..7953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7942..7964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7961..7977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7961..7977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca cagtttgcgt ttttcaggat ctgcagtggg
      601 gcctgttgca attgcttgtg tgggtacaat tcccactgat gtttgcatct gagacatatt
      661 tggcacgttg gtgactgaag tattcttgat atctgtaggg aaggtgggca aactgttgac
      721 catgctctgt ttgctggcta actgggggtt cactccagtg gctcccattg gctgccctcc
      781 agcttgacta aagggctgtc caaatggact tgtgttccca gttattccca tcttggccat
      841 gcctcctgcc tgtgcggtgt tcagtcccgc gtgaccagtc atttgcgggg aaacctgcgt
      901 tagggtctca gccagcacgc tgctcgaggc gccctgcatg gctggagtag ggtacggcat
      961 tccagctccc cttcctctgc cagcagcccc aagagatcca ttcatgactt gcgcctgccc
     1021 ttgtgaagcc tgattaatta agctatggcc agagttacta ttgaggaggc ctgggtgggt
     1081 ctggttaaag ttagcattca tgcagatacc aggtccagtc tgtgacgtgg cagggctgct
     1141 agtcgccagc cccacttgct tttgtgcttg cggattcagt gcttgggagg cagcgggggt
     1201 gggcccagag gtgctggctg cctgtttagg caggctgggg gctgaagaat ctccctggct
     1261 cagagggctc ttgcccatgg cactgaggct ggccatgtta gcactgttcg gctgcccttg
     1321 agcctggcca cccaggccct gctgcacggg gctgctggcg ctcacatttc ctattcctgg
     1381 gttgatacta gagccgctgc ctcctcgtag aagctccgac agttgtttat gtttggaagc
     1441 agcatctgga acaaggttcc cactgtttaa aaggcctaat tctcctccat tgggtatcag
     1501 ctcatcagga agatcatttt ccaagtcaaa caatgatcca aaatctgtgc tgtcattcgc
     1561 cgagaaaccg ggcgagctga gtttggctct tttggggttg ggcggtccgt ccagcaagtt
     1621 ctcagccatc cgggaagcgg ccgcacttcc cccggagcct ccgcttccgc ccttgtacag
     1681 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     1741 gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg
     1801 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     1861 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     1921 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     1981 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     2041 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     2101 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     2161 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     2221 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     2281 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     2341 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg
     2401 tggatcccgg gcccgcggta ccgtcgactg cagaattcgg ttcactaaac gagcccggtt
     2461 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     2521 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     2581 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     2641 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     2701 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     2761 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     2821 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     2881 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2941 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     3001 aactaatgac cccgtaattg attactatta ataactagca cgcgttcgaa gcttgagctc
     3061 ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact
     3121 ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc
     3181 attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt
     3241 gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct
     3301 tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag
     3361 gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca
     3421 gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt
     3481 aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt
     3541 tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata
     3601 ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt
     3661 cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc
     3721 tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact
     3781 ttatatttat ataattcact tctccaattg tccctcatat ctcctcctta tggtgtttta
     3841 ctaaactttt ccatgttcta atcctcatcc tgtctacttg ccacacaatc atcacctgcc
     3901 atctgttttc cataatccct aatgatcttt gcttttcttc ttggcactac ttttatgtca
     3961 ctattatctt gtattactac tgccccttca cctttccaga ggagctttgc tggtcctttc
     4021 caaagtggat ttctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
     4081 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     4141 ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtact
     4201 gctgtcttaa gatgttcagc ctgatctctt acctgtccta taattttctt taattcttta
     4261 ttcatagatt ctactactcc ttgactttgg ggattgtagg gaatttccag gtctgaagat
     4321 cctgctgctt gctgtgcttt tttcttactt ttgttttgct cttcctctat cttgtctaaa
     4381 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     4441 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     4501 atttgtctac agccttctga tgtttctaac aggccagtat taactgcgaa tcgttctagc
     4561 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     4621 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     4681 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     4741 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     4801 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     4861 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     4921 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     4981 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct
     5041 tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctcccatgg
     5101 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     5161 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     5221 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     5281 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     5341 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     5401 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     5461 tcaataatca atgtcgaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5521 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5581 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5641 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5701 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5761 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5821 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5881 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5941 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6001 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6061 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6121 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6181 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6241 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6301 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6361 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6421 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6481 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6541 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6601 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6661 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6721 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6781 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6841 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6901 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6961 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7021 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7081 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7141 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7201 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7261 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7321 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7381 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7441 gcgcgttggc cgattcatta atgcagcgga tccagacatg ataagataca ttgatgagtt
     7501 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7561 tattgcttta tttgtaacca ttataagctg caataaacaa gttagctttt tgcaaaagcc
     7621 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     7681 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     7741 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     7801 atgcggatcc gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7861 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7921 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7981 gattacgcca agcttgggct gcaggtcgag ggatctccat aagagaagag ggacagctat
     8041 gactgggagt agtcaggaga ggaggaaaaa tctggctagt aaaacatgta aggaaaattt
     8101 tagggatgtt aaagaaaaaa ataacacaaa acaaaatat
//
