LOCUS       sequence-487953-        8028 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_039, derived from human fusion protein CREBBP_ZNF384.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8028)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_039
FEATURES             Location/Qualifiers
     source          1..8028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1561..2271,2272..2274,2275..2277))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1603..1624)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1952..1971
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2211..2232
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2274..2283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2359..2562)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2388..2408)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2563..2866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3123..3167)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3352..3585
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3945..4062
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4554..4679
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4726..4983
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4985..5364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5395..5499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5500..5568,5569..6360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5718..5737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6531..7119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7020..7039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7273..7290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7358..7492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7382..7401)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7436..7455
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7498..7693)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7512..7647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7752..7773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7788..7793,7794..7811,7812..7818)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7826..7842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7831..7853
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7850..7866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7850..7866
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gccgtctttc tgatggtcat catcatcctc
      601 aggggagagg acataagggt cattcatctc gggcagccct gattccagca tccgcttctt
      661 cttccggccc cggggtggct taggagccac actgccacct ccaccaccac ctccgcctcc
      721 ttcctcggtt agggtcgatg ctaccttctt ggagaggtca gggacaacct gcagggcttg
      781 tgagccaggg ggaagagctg agacaatcat gggagccgaa atggggaagg tctgagctga
      841 tgatgctgtg gtcacaagag agcctgaggg ggacgtgatt accaaacccg gaccccttga
      901 ttgactccct tctcttctcc acctctgaga acaggagact ccagcagtca tcagtcctgt
      961 agacggcaca gggaccaccg tgatattctg ggtaacggac gcttggctgt gtggggtcag
     1021 ctggtctgac ttggactctg tgtccatact gatgcctgag ggcagggaca ctgaggcagg
     1081 cactgtcagc aaggtggggt agtgaggtgg ggccagacca cagcccttct ctggcaacag
     1141 ctgatccttc atcttgttga tgaacattgt gttctcgatc tgacctgaga ctgtggggat
     1201 agaaggccag aagtacgggt tagaattgaa gtgagattct tccattctac ccagggggtt
     1261 gagagtcctc atctgctggt gggtttgagg ctgtgctggt tgctggccag gaacctgagg
     1321 ctgcagctgc gtctggggct ggttcatgta ggggagtccg agagcagcat aggctcgctg
     1381 catggagctg gggtctatgg gatttgggtt acttaaagaa gtggcattct gttgccctgt
     1441 gccaacagaa ccaattgtgt tttgaattcc actagctgga gaccccagga tggtttgttg
     1501 gtttcgcttg tcactggccc gggaagcggc cgcacttccc ccggagcctc cgcttccgcc
     1561 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     1621 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc tcaggtagtg
     1681 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     1741 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     1801 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     1861 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     1921 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     1981 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     2041 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     2101 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     2161 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     2221 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
     2281 ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt tcactaaacg
     2341 agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     2401 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     2461 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     2521 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     2581 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     2641 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     2701 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2761 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2821 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     2881 tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac gcgttcgaag
     2941 cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     3001 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     3061 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     3121 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     3181 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     3241 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     3301 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     3361 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     3421 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     3481 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     3541 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     3601 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     3661 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctccttat
     3721 ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc cacacaatca
     3781 tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct tggcactact
     3841 tttatgtcac tattatcttg tattactact gccccttcac ctttccagag gagctttgct
     3901 ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
     3961 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     4021 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
     4081 atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat aattttcttt
     4141 aattctttat tcatagattc tactactcct tgactttggg gattgtaggg aatttccagg
     4201 tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc ttcctctatc
     4261 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     4321 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     4381 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt aactgcgaat
     4441 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     4501 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     4561 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     4621 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     4681 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     4741 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     4801 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     4861 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa
     4921 gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc
     4981 ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     5041 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     5101 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     5161 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     5221 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     5281 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     5341 aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga
     5401 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5461 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5521 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5581 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5641 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5701 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5761 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5821 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5881 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5941 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6001 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6061 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6181 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6421 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6481 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6661 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7141 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7201 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7261 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7321 cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga taagatacat
     7381 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7441 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttagcttttt
     7501 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     7561 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     7621 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     7681 ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7741 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7801 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7861 tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata agagaagagg
     7921 gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta aaacatgtaa
     7981 ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatat
//
