LOCUS       sequence-487960-        8124 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_047, derived from human fusion protein CUX1_NUTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8124)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_047
FEATURES             Location/Qualifiers
     source          1..8124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1657..2367,2368..2370,2371..2373))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1699..1720)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2048..2067
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2307..2328
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2370..2379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2455..2658)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2484..2504)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2659..2962
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3219..3263)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3448..3681
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4041..4158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4650..4775
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4822..5079
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5081..5460
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5491..5595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5596..5664,5665..6456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5814..5833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6627..7215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7116..7135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7369..7386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7454..7588
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7478..7497)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7532..7551
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7594..7789)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7608..7743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7848..7869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7884..7889,7890..7907,7908..7914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7922..7938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7927..7949
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7946..7962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7946..7962
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca catgtaaact tcatattgtt ccggggtcag
      601 cggcggcacg ctcggctttg tcggcttgct catcggcacg atgggagagc tcgggagcgg
      661 gctattctgc ggggtttcgc tacggctcgc gccggtgaga gacagttcgc tcgactggct
      721 gtaggggctt tcagctgaag gccactcttt ccagtactca ctgctagaag gaccttgcaa
      781 ctgggatctc tccgcacggg gctggcttcc gccaccgcta ccgcctgagc ccttctcctt
      841 accaccaccc gtctcttctg ccttcgcctc ctcgctagac gccgcgttac gacgttctgg
      901 ttgaaccgcg ctccaccaat gatctttcca cgcaccgcta cgacccactt cattcttcac
      961 ctgacgcaac acaccctgag cacaatccgc agcgccctgc gggtccagtc caggagcgtc
     1021 ttgggcttcc ttctttaatg ccggcggatt cggcagcgcg gacgcacccg cctccggggc
     1081 agcgctcggt tttttgaggg aaatcgccaa aggcgggtag ctgctaacag tcggcatcgg
     1141 ggaggtgctc aacaatttcg gcgtcaagat ggtaatgtcg gattgcgaca gcggtgcctg
     1201 ctttagagcc gggtccagcg ccgcttgctg ggcttccatt tcacgacggg cttgttgcag
     1261 aatactacga atagcgtcat cactgttacc ggagccagat gcactcgacg gttgtgccgg
     1321 ctctgccgtc ttctgaactt gaagctcacg ctttgcctgc tccaggatgc ttttaatggc
     1381 ctcgtcgcta ccggtttcgg aagcgcggat tctggtggta atgttacctt cagaaccgtt
     1441 acggcgcttc ggtacctcct gaaacaggcg gttcaaactc tggcccggat tctcacgctg
     1501 acgtccctgg atgcttctca gcgccagaat gttttgctcg tcgcttaaga actgtttcat
     1561 tttgtggaac ggctccttac cacgaactgt cagcttattc cacggtttcg gccgccggga
     1621 agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt ccatgccgag
     1681 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     1741 gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     1801 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     1861 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     1921 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     1981 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     2041 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     2101 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     2161 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     2221 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     2281 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     2341 cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat cccgggcccg
     2401 cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta aacgagctct
     2461 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     2521 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     2581 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     2641 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     2701 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     2761 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     2821 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     2881 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     2941 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     3001 aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc cctcggggtt
     3061 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     3121 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     3181 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     3241 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     3301 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     3361 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     3421 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     3481 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     3541 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     3601 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     3661 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     3721 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     3781 tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa cttttccatg
     3841 ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg ttttccataa
     3901 tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt atcttgtatt
     3961 actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag tggatttctg
     4021 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     4081 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     4141 cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt cttaagatgt
     4201 tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat agattctact
     4261 actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc tgcttgctgt
     4321 gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     4381 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     4441 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     4501 tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct gcttgcccat
     4561 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     4621 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     4681 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     4741 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     4801 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     4861 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     4921 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     4981 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     5041 agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata gcgatgacta
     5101 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5161 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5221 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5281 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     5341 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     5401 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     5461 gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5821 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6121 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7261 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7321 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7381 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7441 cattaatgca gcggatccag acatgataag atacattgat gagtttggac aaaccacaac
     7501 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7561 aaccattata agctgcaata aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa
     7621 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     7681 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     7741 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgcg gatccgctgg
     7801 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7861 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7921 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     7981 gggctgcagg tcgagggatc tccataagag aagagggaca gctatgactg ggagtagtca
     8041 ggagaggagg aaaaatctgg ctagtaaaac atgtaaggaa aattttaggg atgttaaaga
     8101 aaaaaataac acaaaacaaa atat
//
