LOCUS       sequence-487963-        8100 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_050, derived from human fusion protein DDX3X_MLLT10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8100)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_050
FEATURES             Location/Qualifiers
     source          1..8100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1633..2343,2344..2346,2347..2349))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1675..1696)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2024..2043
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2283..2304
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2346..2355
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2431..2634)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2460..2480)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2635..2938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3195..3239)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3424..3657
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4017..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4626..4751
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4798..5055
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5057..5436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5467..5571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5572..5640,5641..6432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5790..5809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6603..7191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7092..7111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7345..7362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7430..7564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7454..7473)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7508..7527
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7570..7765)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7584..7719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7824..7845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7860..7865,7866..7883,7884..7890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7898..7914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7903..7925
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7922..7938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7922..7938
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca tgttaccggt tgcataccgc tagctaacgc
      601 gcccaccgtc acaccattta cctgttgtac tccgctgaca cccgaccctt gctgtgcggg
      661 tgattgtccc gccgggggag gggtggacag agcgctgcta ctagatgtgc tttgtcccga
      721 agaggtcaga tcttggttaa gaacgggcaa tgaattgtct ggcaaaaagg agtttccaat
      781 atgtggtgac ttcgacgagt ttaaagaatc agtcgtcggt gcagtctgtg cactgaaagt
      841 atgaatttga tgagacggcg acgggtttgc ggtgattgtg gggaaaggca cagaaagctg
      901 agcattaaga agttgcaggc gttccttctt tgctgtcaga tttttgattt gctcttccag
      961 gcgacggttc tccacttgta actggtgcag agatttcaac atacccagaa tgtcggacgg
     1021 ggttccttgt tccagcagaa actgctgtcc ctcgctccat tgacgttcca gcagttgctc
     1081 aatcgatgct gccacgggcg gcaaattttc taatgaactg tttccaggtt gatcgtaacg
     1141 gatttgtaac gaggataccg gagagcgagg tgacaacgaa ccacgggggg aggaaccacg
     1201 tcctactaaa ttacgtgaat tatcgcccag atcctggtct gtctggttgt tctcactttg
     1261 tgcgattaag gcagccatag aactattgga gcggttccca tacatgtggg aaggagcctg
     1321 agaaagtgaa gatcccgaaa gggtgttggc ttgcgtggtt gcaaccgcgg gggccgcaga
     1381 ggagacagca gagggagaca aagcgccaac ctgttgaagg tgaccggagc tctgttgggg
     1441 aaggtggcta gaactaacgg gagtcgaact gcctgagcca gacaccacat tagggaagct
     1501 aacagccaca tcgttcgaat tgtaaatact agaagctgtc gaacccccag attggttgtc
     1561 ggaggagttc agatctaagc cggcgaactg ccgggaagcg gccgcacttc ccccggagcc
     1621 tccgcttccg cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     1681 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgggt
     1741 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     1801 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     1861 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     1921 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     1981 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     2041 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     2101 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     2161 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     2221 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     2281 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     2341 gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt accgtcgact gcagaattcg
     2401 gttcactaaa cgagcccggt tcactaaacg agctctgctt atatagacct cccaccgtac
     2461 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     2521 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     2581 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     2641 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     2701 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
     2761 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
     2821 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
     2881 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
     2941 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagc
     3001 acgcgttcga agcttgagct cggtcccctc ggggttggga ggtgggtctg aaacgataat
     3061 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     3121 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     3181 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     3241 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     3301 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     3361 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     3421 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     3481 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     3541 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     3601 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     3661 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     3721 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
     3781 tctcctcctt atggtgtttt actaaacttt tccatgttct aatcctcatc ctgtctactt
     3841 gccacacaat catcacctgc catctgtttt ccataatccc taatgatctt tgcttttctt
     3901 cttggcacta cttttatgtc actattatct tgtattacta ctgccccttc acctttccag
     3961 aggagctttg ctggtccttt ccaaagtgga tttctgctgt ccctgtaata aacccgaaaa
     4021 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     4081 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     4141 atgaatactg ccatttgtac tgctgtctta agatgttcag cctgatctct tacctgtcct
     4201 ataattttct ttaattcttt attcatagat tctactactc cttgactttg gggattgtag
     4261 ggaatttcca ggtctgaaga tcctgctgct tgctgtgctt ttttcttact tttgttttgc
     4321 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     4381 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     4441 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagta
     4501 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     4561 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     4621 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     4681 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     4741 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     4801 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     4861 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     4921 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     4981 tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg
     5041 cccaggccac gcctcccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5101 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     5161 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     5221 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     5281 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     5341 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     5401 ttgattacta ttaataacta gtcaataatc aatgtcgacc caggtggcac ttttcgggga
     5461 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5521 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5581 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5641 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5701 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5761 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5821 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5881 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5941 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6001 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6061 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6121 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6181 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6541 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6601 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6661 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6721 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6781 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6841 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6901 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6961 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7021 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7081 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7141 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7201 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7261 gcgttatccc ctgcttctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     7321 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcgg atccagacat
     7441 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7501 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     7561 agttagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7621 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     7681 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     7741 tatggttgct gactaattga gatgcggatc cgctggcacg acaggtttcc cgactggaaa
     7801 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7861 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7921 acaggaaaca gctatgacca tgattacgcc aagcttgggc tgcaggtcga gggatctcca
     7981 taagagaaga gggacagcta tgactgggag tagtcaggag aggaggaaaa atctggctag
     8041 taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat
//
