LOCUS       sequence-487964-        8013 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_051, derived from human fusion protein DDX3X_MLLT10; 
            KMT2A_MLLT10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8013)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_051
FEATURES             Location/Qualifiers
     source          1..8013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1546..2256,2257..2259,2260..2262))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1588..1609)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1937..1956
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2196..2217
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2259..2268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2344..2547)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2373..2393)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2548..2851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3108..3152)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3337..3570
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4539..4664
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4711..4968
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4970..5349
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5380..5484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5485..5553,5554..6345)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5703..5722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6516..7104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7005..7024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7258..7275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7343..7477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7367..7386)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7421..7440
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7483..7678)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7497..7632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7737..7758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7773..7778,7779..7796,7797..7803)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7811..7827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7816..7838
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7835..7851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7835..7851
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ggtcaccggc tgcatgccgc ttgccagcgc
      601 ccccacggtc actccattaa cctgttgcac accgctgaca ccagagccct gttgcgccgg
      661 actctgaccg gccggcggcg gcgttgacag cgcgctgcta ctagaggtag actgcccgct
      721 agaggtcaga tcctgattca ggaccgggag gctgttatcg ggcagaaagg aatttccgat
      781 atgcggtgac ttagagctgt tcaggctgtc ggtagtcggc gccgtttgcg cagaaaaggt
      841 gtggatctgg tgggacggcg acgggttcgc ggtaatggtc gggaatggca cggacagttg
      901 cgcgttcagc agctgcagac gctccttctt ggcggtcaag ttcttaatct gctcttccag
      961 tctacgattc tcgacttgca actggtgcag gctcttcagc atgcccagga tgtcgctcgg
     1021 ggtgccttgc tcaagcagaa actgttgccc ttcgctccat tgacgctcca gcaactgctc
     1081 tatagaggca gccaccggcg gcaagttctc cagcgagcta ttacccggtt gatcgtaacg
     1141 gatttgcaac gagctgaccg gagaacgcgg gcttaagctc ccgcgtggtg atgagccgcg
     1201 gccgaccaga tttctggaat tatcacccaa atcctggtcg gtctggttgt tctcgctttg
     1261 ggcgatgagt gctgccatgc tggagttaga acgattgcca tacatgtggc ttggggcctg
     1321 agacaggctg gacccggaca gggtattcgc ctgggtggtc gcaactgcgg gggccgcgct
     1381 gctcacagcg ctcggagaca gcgctccaac ttgttgtaga tgaccagagc tctgctgtgg
     1441 caggtgtgac gaggatactg gggtgctaga gccgctgccg ctaacaacat tcgggaagga
     1501 gacccgggaa gcggccgcac ttcccccgga gcctccgctt ccgcccttgt acagctcgtc
     1561 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     1621 ctcgttgggg tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag
     1681 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     1741 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     1801 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     1861 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     1921 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     1981 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     2041 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     2101 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     2161 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     2221 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc
     2281 ccgggcccgc ggtaccgtcg actgcagaat tcggttcact aaacgagccc ggttcactaa
     2341 acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     2401 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     2461 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     2521 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     2581 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     2641 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     2701 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     2761 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     2821 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     2881 tgaccccgta attgattact attaataact agcacgcgtt cgaagcttga gctcggtccc
     2941 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     3001 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     3061 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     3121 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     3181 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     3241 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     3301 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     3361 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     3421 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     3481 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     3541 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     3601 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     3661 ttatataatt cacttctcca attgtccctc atatctcctc cttatggtgt tttactaaac
     3721 ttttccatgt tctaatcctc atcctgtcta cttgccacac aatcatcacc tgccatctgt
     3781 tttccataat ccctaatgat ctttgctttt cttcttggca ctacttttat gtcactatta
     3841 tcttgtatta ctactgcccc ttcacctttc cagaggagct ttgctggtcc tttccaaagt
     3901 ggatttctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     3961 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     4021 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tactgctgtc
     4081 ttaagatgtt cagcctgatc tcttacctgt cctataattt tctttaattc tttattcata
     4141 gattctacta ctccttgact ttggggattg tagggaattt ccaggtctga agatcctgct
     4201 gcttgctgtg cttttttctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     4261 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     4321 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     4381 ctacagcctt ctgatgtttc taacaggcca gtattaactg cgaatcgttc tagctccctg
     4441 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     4501 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     4561 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     4621 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     4681 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     4741 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     4801 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     4861 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg
     4921 caggatctga gggctcgcca ctccccagtc ccgcccaggc cacgcctccc atggtaatag
     4981 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     5041 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     5101 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     5161 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     5221 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     5281 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     5341 atcaatgtcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5401 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5461 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5521 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5581 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5641 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5701 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5761 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5821 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5881 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5941 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6001 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6061 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6121 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6181 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6241 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6301 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7141 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7201 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7261 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7321 tggccgattc attaatgcag cggatccaga catgataaga tacattgatg agtttggaca
     7381 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7441 tttatttgta accattataa gctgcaataa acaagttagc tttttgcaaa agcctaggcc
     7501 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7561 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     7621 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcgg
     7681 atccgctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7741 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7801 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7861 gccaagcttg ggctgcaggt cgagggatct ccataagaga agagggacag ctatgactgg
     7921 gagtagtcag gagaggagga aaaatctggc tagtaaaaca tgtaaggaaa attttaggga
     7981 tgttaaagaa aaaaataaca caaaacaaaa tat
//
