LOCUS       sequence-488006-        6465 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Expresses human XPO1 with an N-terminal FLAG tag for pull-down 
            assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Wing CE, Fung HYJ, Kwanten B, Cagatay T, Niesman AB, Jacquemyn M, 
            Gharghabi M, Permentier B, Shakya B, Nandi R, Ready JM, Kashyap T, 
            Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM
  TITLE     SINE compounds activate exportin-1 degradation via an allosteric 
            mechanism.
  JOURNAL   bioRxiv [Preprint]. 2024 Oct 11:2024.10.07.617049. doi: 
            10.1101/2024.10.07.617049.
  PUBMED    39416201
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Oct 11:2024.10.07.617049. doi: 
            10.1101/2024.10.07.617049."
COMMENT     Sequence Label: pGL4-CMV-FLAG-XPO1
FEATURES             Location/Qualifiers
     source          1..6465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /note="synthetic polyadenylation signal"
     misc_feature    63..154
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     103..122
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        221..600
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        601..804
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     755..775
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     849..868
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        849..867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             913..936
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4173..4294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4184..4203)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4238..4257
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4542..4559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4713..5301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4793..4812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5501..6292,6293..6361))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctcttaatga tagctagcgg gccagtaccc
      181 aaaaagcggg gggtctggtg gcagtggagg gggatccgtt gacattgatt attgactagt
      241 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      301 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      421 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      481 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      541 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      601 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      661 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      721 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      781 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      841 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      901 ttggtaccaa tggattataa agatgatgat gataaaccag caattatgac aatgttagca
      961 gaccatgcag ctcgtcagct gcttgatttc agccaaaaac tggatatcaa cttattagat
     1021 aatgtggtga attgcttata ccatggagaa ggagcccagc aaagaatggc tcaagaagta
     1081 ctgacacatt taaaggagca tcctgatgct tggacaagag tcgacacaat tttggaattt
     1141 tctcagaata tgaatacgaa atactatgga ctacaaattt tggaaaatgt gataaaaaca
     1201 aggtggaaga ttcttccaag gaaccagtgc gaaggaataa aaaaatacgt tgttggcctc
     1261 attatcaaga cgtcatctga cccaacttgt gtagagaaag aaaaggtgta tatcggaaaa
     1321 ttaaatatga tccttgttca gatactgaaa caagaatggc ccaaacattg gccaactttt
     1381 atcagtgata ttgttggagc aagtaggacc agcgaaagtc tctgtcaaaa taatatggtg
     1441 attcttaaac tcttgagtga agaagtattt gatttctcta gtggacagat aacccaagtc
     1501 aaatctaagc atttaaaaga cagcatgtgc aatgaattct cacagatatt tcaactgtgt
     1561 cagtttgtaa tggaaaattc tcaaaatgct ccacttgtac atgcaacctt ggaaacattg
     1621 ctcagatttc tgaactggat tcccctggga tatatttttg agaccaaatt aatcagcaca
     1681 ttgatttata agttcctgaa tgttccaatg tttcgaaatg tctctctgaa gtgcctcact
     1741 gagattgctg gtgtgagtgt aagccaatat gaagaacaat ttgtaacact atttactctg
     1801 acaatgatgc aactaaagca gatgcttcct ttaaatacca atattcgact tgcgtactca
     1861 aatggaaaag atgatgaaca gaacttcatt caaaatctca gtttgtttct ctgcaccttt
     1921 cttaaggaac atgatcaact tatagaaaaa agattaaatc tcagggaaac tcttatggag
     1981 gcccttcatt atatgttgtt ggtatctgaa gtagaagaaa ctgaaatctt taaaatttgt
     2041 cttgaatact ggaatcattt ggctgctgaa ctctatagag agagtccatt ctctacatct
     2101 gcctctccgt tgctttctgg aagtcaacat tttgatgttc ctcccaggag acagctatat
     2161 ttgcccatgt tattcaaggt ccgtttatta atggttagtc gaatggctaa accagaggaa
     2221 gtattggttg tagagaatga tcaaggagaa gttgtgagag aattcatgaa ggatacagat
     2281 tccataaatt tgtataagaa tatgagggaa acattggttt atcttactca tctggattat
     2341 gtagatacag aaagaataat gacagagaag cttcacaatc aagtgaatgg tacagagtgg
     2401 tcatggaaaa atttgaatac attgtgttgg gcaataggct ccattagtgg agcaatgcat
     2461 gaagaggacg aaaaacgatt tcttgttact gttataaagg atctattagg attatgtgaa
     2521 cagaaaagag gcaaagataa taaagctatt attgcatcaa atatcatgta catagtaggt
     2581 caatacccac gttttttgag agctcactgg aaatttctga agactgtagt taacaagctg
     2641 ttcgaattca tgcatgagac ccatgatgga gtccaggata tggcttgtga tactttcatt
     2701 aaaatagccc aaaaatgccg caggcatttc gttcaggttc aggttggaga agtgatgcca
     2761 tttattgatg aaattttgaa caacattaac actattattt gtgatcttca gcctcaacag
     2821 gttcatacgt tttatgaagc tgtggggtac atgattggtg cacaaacaga tcaaacagta
     2881 caagaacact tgatagaaaa gtacatgtta ctccctaatc aagtgtggga tagtataatc
     2941 cagcaggcaa ccaaaaatgt ggatatactg aaagatcctg aaacagtcaa gcagcttggt
     3001 agcattttga aaacaaatgt gagagcctgc aaagctgttg gacacccctt tgtaattcag
     3061 cttggaagaa tttatttaga tatgcttaat gtatacaagt gcctcagtga aaatatttct
     3121 gcagctatcc aagctaatgg tgaaatggtt acaaagcaac cattgattag aagtatgcga
     3181 actgtaaaaa gggaaacttt aaagttaata tctggttggg tgagccgatc caatgatcca
     3241 cagatggtcg ctgaaaattt tgttccccct ctgttggatg cagttctcat tgattatcag
     3301 agaaatgtcc cagctgctag agaaccagaa gtgcttagta ctatggccat aattgtcaac
     3361 aagttagggg gacatataac agctgaaata cctcaaatat ttgatgctgt ttttgaatgc
     3421 acattgaata tgataaataa ggactttgaa gaatatcctg agcatagaac gaactttttc
     3481 ttactacttc aggctgtcaa ttctcattgt ttcccagcat tccttgctat tccacctaca
     3541 cagtttaaac ttgttttgga ttccatcatt tgggctttca aacatactat gaggaatgtc
     3601 gcagatacgg gcttacagat actttttaca ctcttacaaa atgttgcaca agaagaagct
     3661 gcagctcaga gtttttatca aacttatttt tgtgatattc tccagcatat cttttctgtt
     3721 gtgacagaca cttcacatac tgctggttta acaatgcatg catcaattct tgcatatatg
     3781 tttaatttgg ttgaagaagg aaaaataagt acatcattaa atcctggaaa tccagttaac
     3841 aaccaaatct ttcttcagga atatgtggct aatctcctta agtcggcctt ccctcaccta
     3901 caagatgctc aagtaaagct ctttgtgaca gggcttttca gcttaaatca agatattcct
     3961 gctttcaagg aacatttaag agatttccta gttcaaataa aggaatttgc aggtgaagac
     4021 acttctgatt tgtttttgga agagagagaa atagccctac ggcaggctga tgaagagaaa
     4081 cataaacgtc aaatgtctgt ccctggcatc tttaatccac atgagattcc agaagaaatg
     4141 tgtgattaag ggcccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     4201 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4261 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4321 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     4381 tggtaaaatc gataaagatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct
     4441 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     4501 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
     4561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5461 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
     5521 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
     5581 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
     5641 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
     5701 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
     5761 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
     5821 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
     5881 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
     5941 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
     6001 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
     6061 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
     6121 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
     6181 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
     6241 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
     6301 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
     6361 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
     6421 gataagtaag taatattaag gtacgggagg tattggacag gccgc
//
