LOCUS       sequence-488015-        3276 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Contains the ORF for the homing endonuclease I-SceI under the 
            control of the strong, constitutive yeast promoter PTDH3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3276)
  TITLE     Reddy lab plasmids 2025
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pI-SceI
FEATURES             Location/Qualifiers
     source          1..3276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     273..292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        489..1155
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     1025..1048
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      complement(2108..2142)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2213..2872)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2787..2806
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(3060..3276,1..372)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
       61 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      121 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      181 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      241 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      301 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      361 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      421 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg taacgcagtt
      481 cgagtttatc attatcaata ctgccatttc aaagaatacg taaataatta atagtagtga
      541 ttttcctaac tttatttagt caaaaaatta gccttttaat tctgctgtaa cccgtacatg
      601 cccaaaatag ggggcgggtt acacagaata tataacatcg taggtgtctg ggtgaacagt
      661 ttattcctgg catccactaa atataatgga gcccgctttt taagctggca tccagaaaaa
      721 aaaagaatcc cagcaccaaa atattgtttt cttcaccaac catcagttca taggtccatt
      781 ctcttagcgc aactacagag aacaggggca caaacaggca aaaaacgggc acaacctcaa
      841 tggagtgatg caacctgcct ggagtaaatg atgacacaag gcaattgacc cacgcatgta
      901 tctatctcat tttcttacac cttctattac cttctgctct ctctgatttg gaaaaagctg
      961 aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct acttgactaa taagtatata
     1021 aagacggtag gtattgattg taattctgta aatctatttc ttaaacttct taaattctac
     1081 ttttatagtt agtctttttt ttagttttaa aacaccaaga acttagtttc gaataaacac
     1141 acataaacaa acaaaagatc tatgaagaac atcaagaaga accaagttat gaacttgggt
     1201 ccaaactcta agttgttgaa ggaatacaag tctcaattga tcgaattgaa catcgaacaa
     1261 ttcgaagctg gtatcggttt gatcttgggt gacgcttaca tccgatctcg cgacgaaggt
     1321 aagacctact gtatgcaatt cgaatggaag aacaaggctt acatggacca cgtttgtttg
     1381 ttgtacgacc aatgggtttt gtctccacca cacaagaagg aaagagttaa ccacttgggt
     1441 aacttggtta tcacctgggg tgctcaaacc ttcaagcacc aagctttcaa caagttggct
     1501 aacttgttca tcgttaacaa caagaaaacc atcccaaaca acttggttga aaactacttg
     1561 accccaatgt ctttggctta ctggttcatg gacgacggtg gtaagtggga ctacaacaag
     1621 aactctacca acaagtctat cgttttgaac acccaatctt tcaccttcga agaagttgaa
     1681 tacttggtta agggtttgag aaacaagttc caattgaact gttacgttaa gatcaacaag
     1741 aacaagccaa tcatctacat cgactctatg tcttacttga tcttctacaa cttgatcaag
     1801 ccatacttga tcccacaaat gatgtacaag ttgccaaaca ccatctcttc tgaaaccttc
     1861 ttgaagtaat aactcgagat aaagcaatct tgatgaggat aatgattttt ttttgaatat
     1921 acataaatac taccgttttt ctgctagatt ttgtgatgac gtaaataagt acatattact
     1981 ttttaagcca agacaagatt aagcattaac tttacccttt tctttctaag tttcaatatt
     2041 agttatcact gtttaaaagt tatggcgaga acgtcggcgg ttaaaatata ttaccctgaa
     2101 cgcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     2161 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     2221 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     2281 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     2341 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     2401 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     2461 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     2521 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     2581 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     2641 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     2701 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     2761 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     2821 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2881 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2941 tatggtgaaa gttggaacct cttacgtgcc cgatcaagga tcctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctg
//
