LOCUS       sequence-488038-        9451 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Production of viral vector encoding full-length SHP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   van Vlimmeren AE, Voleti R, Chartier CA, Jiang Z, Karandur D, 
            Humphries PA, Lo WL, Shah NH
  TITLE     The pathogenic T42A mutation in SHP2 rewires the interaction 
            specificity of its N-terminal regulatory domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jul 23;121(30):e2407159121. doi: 
            10.1073/pnas.2407159121. Epub 2024 Jul 16.
  PUBMED    39012820
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-07-23"; volume: "121(30):e2407159121. 
            doi"; pages: " 10.1073/pnas.2407159121. Epub 2024 Jul 16"
COMMENT     Sequence Label: pMSCV-SHP2(T42A) full-length-PIG
FEATURES             Location/Qualifiers
     source          1..9451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     448..467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     701..718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    835..856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(871..876,877..894,895..901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    909..925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             1087..1601
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(join(1703..2416,2417..2419,2420..2422))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1748..1769)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2097..2116
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2356..2377
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    2434..2986
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2436..3003
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2596..2615)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2825..2842
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(3064..3663)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3147..3167)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3644..3663
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3684..4183)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3732..3751)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4125..4148
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5994..6023)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(6042..6458)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6058..6074)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6090..6112)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6525..6866
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             6930..7446
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(7646..7665)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7793..7815)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8009..8028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8128..8150
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8188..8206)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8274..8378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8379..8447,8448..9239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8597..8616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9410..9451,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca caattctccc cagctctttc tgcgctgtcc
      961 ataatgaact atttcaggag tattggagta tgtggattca aaacagtggc taattgagtc
     1021 aggattactt aatattcgac tcctaccctc agaatgttgt tctccaactt caagaaactg
     1081 ttacccatga aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg
     1141 aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc
     1201 gggcgacgca gtctatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt
     1261 gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa
     1321 ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta
     1381 gaaactgctg agggcgggac cgcatctggg gaccatctgt tcttggccct gagccggggc
     1441 aggaactgct taccacagat atcctgtttg gcccatattc tgctgtctct ctgttcctaa
     1501 ccttgatctg aacttctcta ttctcagtta tgtattttcc atgccttgca aaatggcgtt
     1561 acttaagcta gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga
     1621 ctaaataaaa tcttttattt tatcgataag cttggctgca ggtcgacggt atcgataagc
     1681 ttgataattc ttaattaatt cattacttgt acagctcgtc catgccgaga gtgatcccgg
     1741 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     1801 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     1861 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     1921 tcttgaagtt cgccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     1981 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     2041 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     2101 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     2161 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     2221 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     2281 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgcca gacacgctga
     2341 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     2401 gctcctcgcc cttgctcacc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa
     2461 accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta aacacatgta
     2521 aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc
     2581 ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa
     2641 ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt
     2701 tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag ggtcgctaca
     2761 gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac attcaacaga
     2821 ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg
     2881 ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa taacatatag
     2941 acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg gggggggggc
     3001 ggaattggat gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg
     3061 gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
     3121 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
     3181 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
     3241 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
     3301 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
     3361 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
     3421 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
     3481 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
     3541 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
     3601 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
     3661 catggtaagc ttgggctgca ggtcgaaagg cccggagatg aggaagagga gaacagcgcg
     3721 gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc
     3781 gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga
     3841 gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc
     3901 cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt
     3961 cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta
     4021 gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg
     4081 gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa
     4141 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttcttatctg
     4201 aaacttttct gctgttgcat caggcccacg ttttcataga ctctagcact gtcttctctc
     4261 atttctgcac agggtggcgt tggagtacaa ggcgggagag ggctctgatc tccactcgtc
     4321 tggtccgcta gagaatactt aatatttgta tattcgtgcc ctttcctctt gcttttctgc
     4381 tcttcttcaa tcctgcgctg tagtgtttca atataatgct ggaccgccat atagataaat
     4441 cggtactgtg cttctgtctg gaccatccct gacctctgag accgcaccat ctggatggtt
     4501 ttgggaacgt caatatcgca gtcaacacct ttctctctga tgatgtcaat aagaatatca
     4561 atcacaatga acgtccctgt ccggccaatt ccagcactgc agtgcaccac gaccggccct
     4621 gcatccatga tgctctcctg cttatggtgc acctcctcca ggaagtccag cacgccccca
     4681 gggtcgctgg gcacgccgtg gtccggccag gtccgaaagt ggtattgcca gaccgttctc
     4741 tccgtattcc cttgtccaac ctttgaaagt ttaagttctc ttagcgtata gtcatgagcg
     4801 gcgctttctt tgacgttcct aacacgcatg acgccatatt cttttagagc atactcatca
     4861 ggccagtatt tgacacattt actctttcct ctctccactt ctttcgttgt catgacaatc
     4921 actcgggagt tttcttggaa caccatccgc caaaagtcat tcaccgtgtt ttgcaggcag
     4981 ccttgtgtgg caatgtaact ctttttgggc tttgaattgt tgcacttggt ttcaaattca
     5041 ggcatgatga tatttgcatt gatgtaatct gaaacaggct cattgggatc accatcgtgt
     5101 aggacaaccc tggtatgatc aaagggcagg atgtttttat atctattttt gtttttgttt
     5161 tcttgccttt gaccctcttt tcggctgtag agaagtttgc actcctgttg ttgtagtgtc
     5221 tcaaattctt cccaaaagcc ttgtttgact ttatctgtgg tctcagctaa tttgcttagt
     5281 tctcgaactc tgctttctat ttcagcagca tttatacgag tcgtgttaag gggctgcttg
     5341 agttgtagta ctgtacccaa tgtttccacc ataggattct tcttataatg ttccacaaga
     5401 tctgtcaaag aatcaaaccg ttctcctcca ccaacgtcgt atttcagttc ctgacagcga
     5461 atcataacat gggtcacttt agacttgccg tcattgctct cccctttgtc atcaccagtg
     5521 cgcacagaaa gaacaaaatc tccagggtgg ctctggctct ctcgtacaag aaaactacca
     5581 tgttttcctt tttcagttaa taatttctct gcttctttcc cagagagatg tccatgaaac
     5641 cacctttcag aggtaggatc tgcacagttc agaggatatt taagctcaat gacatctcca
     5701 ttcttctctt ttaattgccc gtgatgttcc atgtaatact ggaccaactc agccaaagtg
     5761 gcaaatttct cccctccata caggtcatag taatcaccag tgttctgaat cttgatgtgg
     5821 gtgacagctc catttcttct aacggaaagt gcgaagtctc cagggttact tttactaggc
     5881 cttgccaaaa aactgccatc aactcctctt gtcaacagta ggttttctgc ctccacacca
     5941 gtgatatttg ggtgaaacca tctccgcgat gtcatctgcc cagactgccc tcccagatcc
     6001 tcttctgaga tgagtttttg ttccatggta gatctaattc cggcgcctag agaaggagtg
     6061 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
     6121 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
     6181 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
     6241 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg
     6301 ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa
     6361 cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga
     6421 cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga caaatacaga
     6481 aacacagtca gacagagaca acacagaacg atgctgcagc gctgcagcag acaagacgcg
     6541 cggcttcggt tccaaaccga aagcaaaaat tcagacggag gcgggaactg ttttaggttc
     6601 tcgtctccta ccagaaccac atatcctgac ggggtcggat tccacatcga ctcccttcct
     6661 caggtcgggc cacaaaaacg gcccccaaag tccctgggac gtctcccagg gttgcggccg
     6721 ggtgttcaga actcgtcagt tccaccacgg gtccgccaga tacagagcta gttagctaac
     6781 tagtacagac gcaggcgcaa acattagatg ccggcacaaa cacgcacaaa gacagagaca
     6841 gacacgaaac gaccgctggc cagcttacct cccggcgggg gggtcggtgg tccctgggca
     6901 ggggtctcca aatctcggtg gaacctccaa atgaaagacc cccgaggtgg gcagtcaatc
     6961 aatctgagga gaccctccca aggatcagcg agtccacgat tcggatgcaa acagcaagag
     7021 gctttattgg gaatacgggt acccgggcga cgcagtctat cggaggactg gcgcgccgag
     7081 tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag
     7141 aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc
     7201 ctggaaacat ctgatggttc tctagaaact gctgagggcg ggaccgcatc tggggaccat
     7261 ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat
     7321 attctgctgt ctctctgttc ctaaccttga tctgaacttc tctattctca gttatgtatt
     7381 ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct
     7441 ttcattcccc cctttttctg gagactaaat aaaatctttt attttatcta tggctcgtac
     7501 tctataggct tcagctggtg atattgttga gtcaaaacta gagcctggac cactgatatc
     7561 ctgtctttaa caaattggac taatcgcctc tacgccggac gcatcgtggc cggcatcacc
     7621 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     7681 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     7741 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcgcc gtcgttttac
     7801 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7861 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7921 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7981 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8041 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8101 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     8161 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8221 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9421 tttttctgcg cgtaatctgc tgcttgcaaa c
//
