LOCUS       sequence-488120-        6204 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Golden Gate Level 0 ""SC"" RUBY module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6204)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pL0M-SC-RUBY
FEATURES             Location/Qualifiers
     source          1..6204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             58..4008
                     /label=RUBY
     CDS             1558..1614
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2449..2505
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4048..4066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4134..4238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4460..5251
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5422..6010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5911..5930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6164..6181
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc tcaaatgatg
       61 gatcatgcga ccctcgccat gatcctcgcg atctggttca tcagcttcca cttcatcaag
      121 ctgctgttct cccagcagac caccaagctg cttccgccag gaccaaagcc gcttccgatc
      181 atcggcaaca tccttgaggt gggcaagaag ccgcatcggt ccttcgccaa cctcgccaag
      241 attcacggcc cactcatttc cctcagactc ggctctgtga ccaccatcgt tgtgtcctct
      301 gccgacgtgg ccaaagagat gttcctcaag aaggatcacc cgctctccaa ccgcacgatc
      361 ccgaatagtg ttacagccgg cgaccaccac aagctcacca tgtcttggct cccggtgtct
      421 ccgaagtggc gcaacttccg caagattacc gccgtgcatc tgctctcccc acagagactc
      481 gatgcctgcc agacattcag gcacgccaag gtgcagcagc tctacgagta cgttcaagag
      541 tgcgcccaga aaggccaggc cgtggatatt ggcaaggccg cctttacgac cagcctcaac
      601 ctcctcagca agctgttctt cagcgtcgag ctggcgcacc acaagtccca taccagccaa
      661 gagttcaaag agctgatctg gaacatcatg gaagatatag gcaagccgaa ctacgccgac
      721 tacttcccga ttctcggctg cgttgaccca tctggcatta gaagaaggct cgcctgctcc
      781 ttcgacaagc tgatcgccgt gttccagggc atcatctgcg agagactcgc cccagattcc
      841 tccaccacaa ctaccaccac caccgacgac gtgctcgatg tgctcctcca gctgttcaag
      901 cagaacgagc tgacgatggg cgagatcaac cacctcctcg tggacatctt cgacgccggc
      961 accgatacca catcctccac attcgagtgg gtgatgaccg agctgatccg caatccagag
     1021 atgatggaaa aggcccaaga ggaaatcaag caggtcctcg gcaaggacaa gcagatccaa
     1081 gagtccgaca tcatcaacct gccgtacctc caggcgatca tcaaagagac actccgcctc
     1141 catccgccga ccgtgttctt gctcccaaga aaggccgaca ccgatgtcga gctgtacggc
     1201 tacatcgtgc cgaaggatgc ccagatcctc gtgaacctct gggccattgg cagggaccca
     1261 aacgcctggc agaacgccga tattttcagc ccagagcgct tcatcggctg cgagatcgat
     1321 gttaagggcc gcgatttcgg cctccttcca tttggcgctg gccgcagaat ttgcccaggc
     1381 atgaatctcg ccatcaggat gctcaccctc atgctcgcca cactcctcca gttcttcaac
     1441 tggaagctcg aaggcgacat ctccccgaag gacctcgaca tggacgagaa gttcggcatt
     1501 gcgctccaaa agaccaagcc gctcaagctc atcccgattc cgcgctacgg tagcggagct
     1561 accaatttta gcctccttaa gcaggcaggt gatgtagaag agaaccccgg gcctatgaag
     1621 atgatgaacg gcgaggacgc caacgaccag atgatcaaag agtccttctt catcacccac
     1681 ggcaacccga tcctcaccgt cgaggataca catccgctca ggccgttctt cgagacatgg
     1741 cgcgagaaga ttttctccaa gaagccgaag gccatcctca tcatctccgg ccactgggag
     1801 acagtgaagc caaccgtgaa cgccgtgcac atcaacgaca ccatccacga cttcgacgac
     1861 tacccagccg ccatgtacca gttcaagtac ccagctccag gcgagccaga gcttgcgaga
     1921 aaggtggaag agatcctcaa gaagtccggg ttcgagacag ccgagacaga ccaaaagagg
     1981 ggccttgatc acggcgcctg ggttccactc atgctcatgt atccagaggc ggacatcccg
     2041 gtgtgccagc tctcagttca gccacatctc gacggcacct accactacaa tctcggcaga
     2101 gccctcgcgc cgctcaagaa tgatggcgtg ctcattattg gctccggcag cgccacacat
     2161 ccactcgatg agacaccgca ctacttcgat ggtgttgccc cttgggccgc tgccttcgat
     2221 tcttggctta ggaaggccct catcaacggc cgcttcgagg aagtgaacat ctacgagagc
     2281 aaggccccga actggaagct cgcccatcca tttccagagc acttctaccc gctccacgtt
     2341 gtgctcggcg ctgctggtga aaagtggaag gccgagctga tccactcctc ctgggatcat
     2401 ggcacacttt gccacggctc ctacaagttc acctccgccg gatccggagc aaccaacttt
     2461 agcctgctca agcaagcagg agatgttgag gaaaatcctg gccccatgac cgccatcaag
     2521 atgaacacca acggcgaggg cgagacacag cacatcctca tgatcccgtt catggcgcag
     2581 ggccacctca ggccatttct cgaactcgcc atgttcctct acaagcgctc ccacgtgatc
     2641 atcaccctgc tcacaactcc gctcaatgcc ggcttcctca ggcacctcct tcaccaccat
     2701 tcctactcct ccagcggcat caggatcgtc gagctgccat tcaactccac caaccacgga
     2761 ctcccaccgg gcatcgagaa caccgataag ctcacactcc cgctcgtggt gtccctcttc
     2821 cattccacca tcagcctcga tccgcacctc cgcgattaca tctccaggca tttcagccca
     2881 gccaggccac cactctgcgt gatccatgat gtgttcctcg gctgggttga ccaggtggcc
     2941 aaggatgtgg gctctacagg cgtggtgttc acaacaggcg gcgcttatgg cacatccgcc
     3001 tacgtgtcca tctggaacga tctcccgcac cagaactact ccgacgacca agagttcccg
     3061 ctgccaggct tcccagagaa ccataagttc cgcaggtccc agctccatcg gttcctcaga
     3121 tatgccgacg gctccgacga ttggtccaag tatttccagc cgcagctccg ccagtccatg
     3181 aagtcttttg gctggctctg caactccgtg gaagagatcg agacactcgg cttctccatc
     3241 ctccgcaact acaccaagct gccgatctgg ggcatcggcc cacttattgc ttccccagtg
     3301 cagcactcct cctccgacaa caattcaaca ggcgccgagt tcgtgcagtg gctcagcctc
     3361 aaagagccgg actccgtcct ctacatctcc ttcggctccc agaacacgat cagcccgacg
     3421 cagatgatgg aactcgctgc tggccttgag tcctccgaga agccattcct ctgggtgatc
     3481 agagccccgt tcggcttcga catcaacgaa gagatgcgcc cagagtggct gccagagggc
     3541 tttgaggaac gcatgaaggt gaagaaacag ggcaagctcg tgtacaagct cggcccgcag
     3601 cttgagatcc tcaaccatga atccatcggc ggctttctca cccactgcgg atggaacagc
     3661 atccttgagt ctcttcgcga gggcgttccg atgcttggat ggccacttgc tgccgagcag
     3721 gcctacaacc tcaagtacct cgaagatgag atgggcgtcg cggttgagct tgctagaggc
     3781 ctcgaaggcg agatctccaa agagaaggtc aagcgcatcg tcgagatgat ccttgagcgc
     3841 aacgagggct ccaaaggctg ggagatgaag aatcgcgccg tggaaatggg caaaaagctc
     3901 aaggacgccg tgaacgagga aaaagagctg aagggctcct ccgtgaaggc gatcgacgat
     3961 ttcctcgacg ccgtcatgca ggccaaactt gagccaagcc tccagtgagc tttgagacca
     4021 cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4081 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca
     4261 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     4321 actgtttttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     4381 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     4441 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     4501 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     4561 cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt
     4621 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     4681 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     4741 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     4801 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     4861 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     4921 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     4981 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     5041 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     5101 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     5161 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa
     5221 aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact catatatact
     5281 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5341 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5401 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5461 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5521 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5581 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5641 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5701 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5761 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5821 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5881 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5941 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6001 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6061 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6121 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6181 ggaagcggaa gagcgcccaa tacg
//
