LOCUS       sequence-488128-        8412 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_103, derived from human fusion protein MEF2D_BCL9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8412)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_103
FEATURES             Location/Qualifiers
     source          1..8412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1945..2655,2656..2658,2659..2661))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1987..2008)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2336..2355
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2595..2616
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2658..2667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2743..2946)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2772..2792)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2947..3250
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3507..3551)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4329..4446
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4938..5063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5110..5367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5369..5748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5779..5883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5884..5952,5953..6744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6102..6121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6915..7503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7404..7423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7657..7674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7742..7876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7766..7785)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7820..7839
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7882..8077)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7896..8031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    8136..8157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8172..8177,8178..8195,8196..8202)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8210..8226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8215..8237
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8234..8250
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8234..8250
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca aaacatcatg tttccgggat tgcccggtcc
      601 ttgtgaaaac ccacccattc ccacatctgc tgccatgcca cggggacgca tctgaggagg
      661 gatcataatg ttttgttggg gtcccatcat tccctgcatg ctcatcatca tacctggact
      721 acccacaggt ccgggatgcg tatacagtcc agcaggtcca cgatctttgc cgggaatcat
      781 ccccacggcg gcggctggat tactcataag cgtgggttgc ccaggcatgg tcgattgcgc
      841 cggagacatc atacgatgat gtggtcccat catcccctgc tgcaagaacg ctggcggacg
      901 catagggttg tggcctggca tacttggagc cgtaccaagt gggatgtcgg gcgtcccgac
      961 tgggcccgga ccacccattc ccggtaaagc caaacccata cggggtgcct gctctcccat
     1021 cattccttgc atgtgggaaa accctggtcc gggcccctgg ggttgtttgc gaccagggac
     1081 ctcgccgcga gggaagtact gcagcgtctg tgagggtttc tctgacggaa taatacgaga
     1141 aagatcaaac tcaggaatgc cggtagcacc gggacgaatt acttcttgca aatcgggatc
     1201 agtaaacacg gatggcatgc tgttccccaa gacggtgaaa ctatccggcc ctccgggacc
     1261 tcccggttta cacagcgcag catcagctga actctgcggc aagtttgatg ggcgacccag
     1321 tggcccttca cccggaaatc ccatgccacc aggaaatgag ccttggcccc cggagggccc
     1381 gttgtgggga aaggggacct gttgcggtgg tgactgcacc ggaggaaagc cctgtggaaa
     1441 gcccatgcgc ccctgaggga ccataggagg ttcctgtgat ccatgtccca taattggatt
     1501 atgactcatt aagcctggcc ccattggcac tccgtgggga gggatattcg ggccaacggc
     1561 gtttggcgag ggcatttgat tggaatggct taatggctgc gtcattccca taggggaaag
     1621 tgtgggcata ggaactactg ggttaggacc cactggggat gggcaagcgc catttgcgct
     1681 attcaagtct ccaccaagca ttgcccccgc cgaggctggg cgttgcggta aacctggtga
     1741 gacactatta cgttgaagtg cgggctgctg tggcgacaac aagcgtgggt ccgtaaggga
     1801 cgaggttacc agtgagggag tgactaagct gccagacggg ttactgaact gaaggcttga
     1861 ttggttagag accgggacgg tcacgggcat cgcgaagttg ggccgggaag cggccgcact
     1921 tcccccggag cctccgcttc cgcccttgta cagctcgtcc atgccgagag tgatcccggc
     1981 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     2041 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     2101 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     2161 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     2221 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     2281 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     2341 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     2401 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     2461 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     2521 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     2581 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     2641 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga
     2701 ctgcagaatt cggttcacta aacgagcccg gttcactaaa cgagctctgc ttatatagac
     2761 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     2821 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     2881 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     2941 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     3001 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     3061 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     3121 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     3181 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     3241 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     3301 ttaataacta gcacgcgttc gaagcttgag ctcggtcccc tcggggttgg gaggtgggtc
     3361 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     3421 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     3481 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     3541 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     3601 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     3661 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     3721 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     3781 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     3841 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     3901 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     3961 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     4021 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     4081 ttgtccctca tatctcctcc ttatggtgtt ttactaaact tttccatgtt ctaatcctca
     4141 tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc cctaatgatc
     4201 tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac tactgcccct
     4261 tcacctttcc agaggagctt tgctggtcct ttccaaagtg gatttctgct gtccctgtaa
     4321 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     4381 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     4441 ttaaaattgt ggatgaatac tgccatttgt actgctgtct taagatgttc agcctgatct
     4501 cttacctgtc ctataatttt ctttaattct ttattcatag attctactac tccttgactt
     4561 tggggattgt agggaatttc caggtctgaa gatcctgctg cttgctgtgc ttttttctta
     4621 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     4681 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     4741 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     4801 aacaggccag tattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     4861 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
     4921 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
     4981 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
     5041 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
     5101 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     5161 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     5221 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     5281 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     5341 tccccagtcc cgcccaggcc acgcctccca tggtaatagc gatgactaat acgtagatgt
     5401 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     5461 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     5521 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5581 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     5641 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     5701 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga cccaggtggc
     5761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5821 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5881 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5941 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6001 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6061 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6121 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6181 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6241 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6301 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6361 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6421 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6481 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6541 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6601 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6661 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6721 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6781 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6841 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6901 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6961 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7021 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7081 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7141 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7201 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7261 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7321 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7381 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7441 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7501 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7561 atgttctttc ctgcgttatc ccctgcttct gtggataacc gtattaccgc ctttgagtga
     7621 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7681 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7741 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7801 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7861 ctgcaataaa caagttagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     7921 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     7981 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     8041 taggggcggg actatggttg ctgactaatt gagatgcgga tccgctggca cgacaggttt
     8101 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8161 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8221 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg gctgcaggtc
     8281 gagggatctc cataagagaa gagggacagc tatgactggg agtagtcagg agaggaggaa
     8341 aaatctggct agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa aaaataacac
     8401 aaaacaaaat at
//
