LOCUS       sequence-488129-        8082 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_104, derived from human fusion protein MEF2D_DAZAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8082)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_104
FEATURES             Location/Qualifiers
     source          1..8082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1615..2325,2326..2328,2329..2331))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1657..1678)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2006..2025
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2265..2286
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2328..2337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2413..2616)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2442..2462)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2617..2920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3177..3221)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3406..3639
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3999..4116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4608..4733
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4780..5037
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5039..5418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5449..5553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5554..5622,5623..6414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5772..5791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6585..7173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7074..7093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7327..7344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7412..7546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7436..7455)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7490..7509
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7552..7747)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7566..7701
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7806..7827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7842..7847,7848..7865,7866..7872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7880..7896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7885..7907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7904..7920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7904..7920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gcgacgatag ggatggaagc cctgaacatt
      601 gtggttctgc ccacggccaa aaccactacc gcctgcaggc ggaccccctg agccaggaac
      661 agaggggcca ccataactcg gaggttgctg ggatggatca ctgaagcctt ggccgaaacc
      721 tgataagtcc tgtccgtaac cggcatactg cccataaggg aagtctggct gcgctgttgg
      781 tggcttggac atgtcggggg cagcctgact aggaggcggc ggaaaggcca aaggtgccgc
      841 acctggggta gctggtggag gtgggacacc tggcggggca aattggtcgg gtggtggcgg
      901 gggtggccca tagccaaaac taaactgcgg gggagcgcca tacccctgag ggaacccctg
      961 gggaggtgga aaccctcctg ggggcgtaga gacgatataa gacgtaaatg gtggtggagg
     1021 cggcggcgcg ccacgtcctg cgggcggggg accatagccc ccgatggctt gtccagcggg
     1081 tacccacatt ccctgtggac cgtagccttg ctgccaggtc ggaggtggtt gacccgccca
     1141 accatttgcg gcgttgggaa cgacacgtga cccccactga cttgcaccgg gttgaccggg
     1201 ggcctgactc tttgagtcgc gtggttcggc acgcttaact tcaacctttt tccccatgat
     1261 gtcgtggaag tgcatgttca cggcttggtc caccgattgc tcgtcctcaa aagtaatgaa
     1321 tccgaagccg acgggcgacg ggcaagcacc gttagcggag ttcaaatccc ctcctaacat
     1381 cgctcctgca ctagctggac gttgaggtaa cccaggggac acgctgttgc gttgtaatgc
     1441 aggctgctgc ggtgacagca aacgaggatc ggtaaggctt gaagtgacca gcgacggggt
     1501 caccaaactc cctgatggat tactaaattg aagggaactc tggttactaa ctggtacggt
     1561 tacgggcatc gcccgggaag cggccgcact tcccccggag cctccgcttc cgcccttgta
     1621 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     1681 atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc
     1741 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     1801 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     1861 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     1921 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     1981 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     2041 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     2101 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     2161 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     2221 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     2281 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
     2341 cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cggttcacta aacgagcccg
     2401 gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     2461 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     2521 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     2581 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     2641 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     2701 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     2761 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     2821 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     2881 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     2941 atgaactaat gaccccgtaa ttgattacta ttaataacta gcacgcgttc gaagcttgag
     3001 ctcggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     3061 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     3121 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     3181 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     3241 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     3301 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     3361 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     3421 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     3481 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     3541 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     3601 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     3661 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     3721 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc ttatggtgtt
     3781 ttactaaact tttccatgtt ctaatcctca tcctgtctac ttgccacaca atcatcacct
     3841 gccatctgtt ttccataatc cctaatgatc tttgcttttc ttcttggcac tacttttatg
     3901 tcactattat cttgtattac tactgcccct tcacctttcc agaggagctt tgctggtcct
     3961 ttccaaagtg gatttctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     4021 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     4081 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     4141 actgctgtct taagatgttc agcctgatct cttacctgtc ctataatttt ctttaattct
     4201 ttattcatag attctactac tccttgactt tggggattgt agggaatttc caggtctgaa
     4261 gatcctgctg cttgctgtgc ttttttctta cttttgtttt gctcttcctc tatcttgtct
     4321 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     4381 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     4441 agtatttgtc tacagccttc tgatgtttct aacaggccag tattaactgc gaatcgttct
     4501 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     4561 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     4621 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     4681 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     4741 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     4801 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     4861 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     4921 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct
     4981 gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccca
     5041 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     5101 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     5161 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     5221 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     5281 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     5341 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     5401 tagtcaataa tcaatgtcga cccaggtggc acttttcggg gaaatgtgcg cggaacccct
     5461 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5521 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5581 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5641 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5701 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5761 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5821 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5881 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5941 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6001 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6061 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6121 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6181 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6241 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6301 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6361 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6421 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6481 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6541 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6601 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7201 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgcttct
     7261 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7321 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7381 cccgcgcgtt ggccgattca ttaatgcagc ggatccagac atgataagat acattgatga
     7441 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7501 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttagct ttttgcaaaa
     7561 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     7621 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7681 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     7741 gagatgcgga tccgctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7801 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7861 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7921 catgattacg ccaagcttgg gctgcaggtc gagggatctc cataagagaa gagggacagc
     7981 tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat gtaaggaaaa
     8041 ttttagggat gttaaagaaa aaaataacac aaaacaaaat at
//
