LOCUS       sequence-488131-        7602 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_108, derived from human fusion protein MFF_ERBB4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_108
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1135..1845,1846..1848,1849..1851))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1177..1198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1526..1545
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1785..1806
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1848..1857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1933..2136)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1962..1982)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2137..2440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2697..2741)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2926..3159
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3519..3636
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4128..4253
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4300..4557
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4559..4938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4969..5073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5074..5142,5143..5934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5292..5311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6105..6693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6594..6613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6847..6864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6932..7066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6956..6975)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7010..7029
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7072..7267)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7086..7221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7326..7347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7362..7367,7368..7385,7386..7392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7400..7416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7405..7427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7424..7440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7424..7440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca caggtaaagg ggctcgttga cgtactcatc
      601 ttccgcctta ggcggcccgt tagacgcatt gtggtattct ggattgtcta acgcctgcag
      661 atccccattt ttacgacggg atacgaaggg attttcctct acaggattca ggtattcttg
      721 tttcggcttg tcacgcattg gtgtcatgta gccctcctcg tccagctccc cacgcggact
      781 acgctccggt gcgaaaaccg tgggatctgc actgtaacgc tgtgtgctag aatcttcctg
      841 aacgtgaggc gcgactggct tgcgcaaagt cccattgcag caagaatcat caaaaatctc
      901 agctgtggcc ccttgtgcaa ctggcgcctc agggatagta ctagtaggag cgcggtaggg
      961 cactgagact ccctgttccg ccgcgaaccc cccatcacgg tagacaaatt ggttacctga
     1021 catgggagta taggcgggcg gaggcgagtg cccaatttcc gagcgattgg aatcaatgcg
     1081 cgcgcgacta gtccgggaag cggccgcact tcccccggag cctccgcttc cgcccttgta
     1141 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     1201 atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc
     1261 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     1321 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     1381 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     1441 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     1501 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     1561 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1621 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     1681 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1741 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1801 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
     1861 cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cggttcacta aacgagcccg
     1921 gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     1981 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     2041 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     2101 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     2161 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     2221 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     2281 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     2341 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     2401 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     2461 atgaactaat gaccccgtaa ttgattacta ttaataacta gcacgcgttc gaagcttgag
     2521 ctcggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     2581 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     2641 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     2701 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     2761 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     2821 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     2881 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     2941 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     3001 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     3061 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     3121 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     3181 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     3241 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc ttatggtgtt
     3301 ttactaaact tttccatgtt ctaatcctca tcctgtctac ttgccacaca atcatcacct
     3361 gccatctgtt ttccataatc cctaatgatc tttgcttttc ttcttggcac tacttttatg
     3421 tcactattat cttgtattac tactgcccct tcacctttcc agaggagctt tgctggtcct
     3481 ttccaaagtg gatttctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     3541 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     3601 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     3661 actgctgtct taagatgttc agcctgatct cttacctgtc ctataatttt ctttaattct
     3721 ttattcatag attctactac tccttgactt tggggattgt agggaatttc caggtctgaa
     3781 gatcctgctg cttgctgtgc ttttttctta cttttgtttt gctcttcctc tatcttgtct
     3841 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     3901 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     3961 agtatttgtc tacagccttc tgatgtttct aacaggccag tattaactgc gaatcgttct
     4021 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     4081 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     4141 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     4201 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     4261 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     4321 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     4381 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     4441 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct
     4501 gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccca
     4561 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     4621 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     4681 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     4741 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     4801 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     4861 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     4921 tagtcaataa tcaatgtcga cccaggtggc acttttcggg gaaatgtgcg cggaacccct
     4981 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5041 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5101 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5161 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5221 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5281 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5341 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5401 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5461 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5521 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5581 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5641 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5701 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5761 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5821 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5881 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5941 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6241 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6721 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgcttct
     6781 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6841 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6901 cccgcgcgtt ggccgattca ttaatgcagc ggatccagac atgataagat acattgatga
     6961 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7021 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttagct ttttgcaaaa
     7081 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     7141 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7201 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     7261 gagatgcgga tccgctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7321 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7381 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7441 catgattacg ccaagcttgg gctgcaggtc gagggatctc cataagagaa gagggacagc
     7501 tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat gtaaggaaaa
     7561 ttttagggat gttaaagaaa aaaataacac aaaacaaaat at
//
