LOCUS       sequence-488146-        9318 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_123, derived from human fusion protein NUP98_NSD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9318)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_123
FEATURES             Location/Qualifiers
     source          1..9318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(2851..3561,3562..3564,3565..3567))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2893..2914)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3242..3261
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3501..3522
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3564..3573
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3649..3852)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3678..3698)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3853..4156
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4413..4457)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4642..4875
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5235..5352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5844..5969
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6016..6273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        6275..6654
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6685..6789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6790..6858,6859..7650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7008..7027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7821..8409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8310..8329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8563..8580
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    8648..8782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8672..8691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8726..8745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8788..8983)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8802..8937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    9042..9063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9078..9083,9084..9101,9102..9108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9116..9132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9121..9143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9140..9156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9140..9156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca cttgagtgca gctcctccac cgcgttcacc
      601 ctgcattttt ttagaggcag gcatcccggg atcgtgttct acaccttcct caacagtctc
      661 cttggggctt gtggccgtcc tgtgaggcat tagttctccc tctgagcctg gaatctcttt
      721 tgacgagtca tcatttttca cttttttaca ctgcatcttg gctgcagcat gcctctgtcg
      781 ttttcgcttc cttggcttgt caaatatctt ggtagtgcct ccaccaaaat cttttgaata
      841 agatgtggca caagattcag ttatgccatt ctccaggtga ccagcttcat agcacttttt
      901 agaaaggcca aggtccccct tcttgggcat aaatccaggg tctaaatcat tggattcaag
      961 aagtttctta gttggttttc tttgacgttt actttcataa tttcctggcg ttttaactag
     1021 caattcctca gactcaaggg caggccgtgg agagacttcc ggagccacag gcacagacaa
     1081 ggtcagctgc ggcaactcag catgtccacc tccaagttct gactgagcca aggggccctc
     1141 caaaaacgga gcctcccttt caagaactgg aggctcttct ttggttgaaa tcaaagaatt
     1201 ctcgtccacc tgcttgttct gagcactaga tcgacctcgg gctagaaggc tttcctcttc
     1261 acagcgggaa cttaccttgt gcacctgctc ctgtaccttc ttttgttttt tcttaggagc
     1321 aaatatctga tcatattctt ctgtatattc caaaagccac ttgcttggct tcctaaggcg
     1381 tttcttctct gaccgcacag ctactggggc ctggggggct ccaaagccca aagtggctgt
     1441 cgtagtatta aatccagggg ccccaaaggc tcctgtacca agaggccctc caatcttagg
     1501 ttggttgttc ccaaacaaag atgcctgtcc agcaccaaga gctgttccaa atcctgcacc
     1561 aagcccagtt ccaagagtcc caggtgctgg tttacttcca aaaatactat tcccactggt
     1621 atttgtgcca aaaccaaaga gcccgccact ggttgtacca aatgaaggtg cactggttgt
     1681 gctgcttcca aatgtagtaa gcttgttatt gccaaacagg gtcgaaccaa cagcaccaaa
     1741 tccagtattg gtctgcccaa agagacctgt tcctgttcca aatgctgtcc cagtgctggt
     1801 gtttgtagct gtcccaaaaa gacctccagg ctgtgaggct tgggttactc caaataatcc
     1861 catggtgttg gtgcttggct gtcctatggt gctggtatta ccaaaggaaa agccagtgtt
     1921 ctgggtggtt gtagcctggc caaatggttt gctgaagagg ctggtagtct gctgattctg
     1981 ttggccaaag agaccacctg gatttgttcc aaatccagtt gtactagttc caaaggcagt
     2041 tttgttctga ccatatgcaa agcctgaatt agtggtggag gagctgaaga gtcctgttgc
     2101 gctggaagtg gctggagaag acccaaacaa gccagttgtg gtacctgctc ccacctggtt
     2161 ctgtgggccc ttcctgttag cctgataatc ctctaaacga agttcctcta gtgacttgct
     2221 ttcatattct ttcatagcag taatacactg gtgcttggta cttatgttag tgctaactcc
     2281 agctttgacc atagtatctg taccagttgg agggttaaat ttaatagtag tcccagtagg
     2341 agcagctgta aaactacttg gcccaaagag ggagccagat gtgctgccaa aaggattaga
     2401 ggtggtattt gtggttccaa agagtcctcc actgctagta ctggttccaa aattgccaaa
     2461 gccagttggt ttattttgtg caaaggcatt gttttgggat gagaagagac tggtccctgt
     2521 gcttgcagtt ccaaacaagg tatttgctgt tcctgttgac gtaccaaacc caaagccagt
     2581 gcttgtggag gtagctggct ggctaaatga actggttcca aacaatcctc ctggtttagt
     2641 ctgtgaattt ccaaagaggc ctccagtatt gttgctagaa ccaaatgcag atgttccaaa
     2701 tgcccctcca ctagtagtgc caaagccagt attctgtcca aatgttgaag ttgtgccaaa
     2761 gccacctgtg ccacccccaa agggtgttcc aaatgatttg ttaaacatcc gggaagcggc
     2821 cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat
     2881 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     2941 gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     3001 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     3061 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     3121 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     3181 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     3241 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     3301 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
     3361 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     3421 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     3481 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     3541 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcggtac
     3601 cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag ctctgcttat
     3661 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     3721 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     3781 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     3841 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     3901 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     3961 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     4021 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     4081 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     4141 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     4201 ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg
     4261 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     4321 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     4381 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     4441 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     4501 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     4561 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     4621 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     4681 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     4741 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     4801 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     4861 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     4921 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     4981 ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc catgttctaa
     5041 tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc ataatcccta
     5101 atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg tattactact
     5161 gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt tctgctgtcc
     5221 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
     5281 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
     5341 tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag atgttcagcc
     5401 tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc tactactcct
     5461 tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg ctgtgctttt
     5521 ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     5581 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     5641 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     5701 gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     5761 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     5821 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     5881 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     5941 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     6001 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     6061 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     6121 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     6181 ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct
     6241 cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg actaatacgt
     6301 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     6361 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     6421 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     6481 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     6541 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     6601 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcgaccca
     6661 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6721 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6781 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6841 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6901 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6961 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7021 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7081 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7141 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7201 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7261 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7321 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7381 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7441 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7501 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7561 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7621 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7681 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7741 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7801 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7861 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7921 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7981 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8041 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8101 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8161 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8221 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8281 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8341 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8401 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8461 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8521 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8581 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8641 tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     8701 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     8761 tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     8821 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     8881 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     8941 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg ctggcacgac
     9001 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9061 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9121 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg
     9181 caggtcgagg gatctccata agagaagagg gacagctatg actgggagta gtcaggagag
     9241 gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa
     9301 taacacaaaa caaaatat
//
