LOCUS       sequence-488147-        8352 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_124, derived from human fusion protein NUP98_NSD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8352)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_124
FEATURES             Location/Qualifiers
     source          1..8352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1885..2595,2596..2598,2599..2601))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1927..1948)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2276..2295
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2535..2556
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2598..2607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2683..2886)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2712..2732)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2887..3190
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3447..3491)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3676..3909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4269..4386
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4878..5003
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5050..5307
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5309..5688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5719..5823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5824..5892,5893..6684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6042..6061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6855..7443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7344..7363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7597..7614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7682..7816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7706..7725)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7760..7779
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7822..8017)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7836..7971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    8076..8097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8112..8117,8118..8135,8136..8142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8150..8166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8155..8177
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8174..8190
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8174..8190
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ggcttttccc agggcccagg gactttgctc
      601 tgtggttacc aacttctttt cttcagtggg gagggaggct ggggccttcc cgagagacct
      661 aaaagattgc ccactcttgg cggcaagtgc aggtagttgc tggcctccga ccatctgctt
      721 cgcctgcttc accaggcccg gggattggct aagaggccct ggggtctgct cagcccctgc
      781 agaagctctt cctgaggcct gagttgttgg ctcatataaa aaggccttgg caggaggctg
      841 agaaagaagt ctggcctggg tgagtgattt aacagcttgc caggggtcct ctgaaggggc
      901 tgctgctttc ccagatgtct gaagaggatc tgacacacag cctttctcaa cagctctcgg
      961 catatgcccc agacctttgc tggcaggcca tgaacttgac tccaacactg gcatcttctc
     1021 atcagcctgt ggtgtgacct gatgaggtga agctgcccgc tccttctggc gaggagttag
     1081 gtctatgaga tccatcacca aagcagctct attttttgac tgagtattct ggtccacagg
     1141 cctcagtgct ttttctttag ctacaagggt ctggaccaca gctgatagta ctttctcagg
     1201 aggtgggagt ctctgggaca aagaggcatg gggtttgtca gtaggcctgt ctgaagtctg
     1261 gggtttggga ctgctagtaa tgagcagtct gtctggcggc ggaagtctca ggccagtggg
     1321 aactgaggtt ttctccagtg actggggcct tgggctggca gcacctatgg atttatccag
     1381 ccttgggtca gccgtcccca gaggtctttc cagtggttgg gacctgactg agggtgagga
     1441 gcttgggctg gtcactggag agggtttgtc gcttagctgg ggtcttggtc ctgccactgc
     1501 tggtggcctg tccagtggcc tctggctgga aaccaaggat tgggatttgg tccctgaccc
     1561 agcgaggtct ctgaccttat ctaaaggctg gggcctggag tcagttctct caagaggtct
     1621 ttcaggtagc aatggcctct gacaagtccc tgccagagct tttttggaga gcgacagcat
     1681 ctggttggtg tcagcaggag gtttatctga cattttgggt gcctgagcag ccattcctgt
     1741 tgattgctct gccaggtgag tgcttggccc tggaggcagc ggtactgggg gaggcacata
     1801 ctcacggatc tccccaggtt ccagaggatt gggcccacag ggccgggaag cggccgcact
     1861 tcccccggag cctccgcttc cgcccttgta cagctcgtcc atgccgagag tgatcccggc
     1921 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     1981 cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     2041 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     2101 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     2161 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     2221 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     2281 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     2341 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     2401 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     2461 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     2521 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     2581 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga
     2641 ctgcagaatt cggttcacta aacgagcccg gttcactaaa cgagctctgc ttatatagac
     2701 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     2761 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     2821 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     2881 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     2941 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     3001 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     3061 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     3121 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     3181 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     3241 ttaataacta gcacgcgttc gaagcttgag ctcggtcccc tcggggttgg gaggtgggtc
     3301 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     3361 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     3421 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     3481 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     3541 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     3601 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     3661 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     3721 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     3781 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     3841 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     3901 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     3961 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     4021 ttgtccctca tatctcctcc ttatggtgtt ttactaaact tttccatgtt ctaatcctca
     4081 tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc cctaatgatc
     4141 tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac tactgcccct
     4201 tcacctttcc agaggagctt tgctggtcct ttccaaagtg gatttctgct gtccctgtaa
     4261 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     4321 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     4381 ttaaaattgt ggatgaatac tgccatttgt actgctgtct taagatgttc agcctgatct
     4441 cttacctgtc ctataatttt ctttaattct ttattcatag attctactac tccttgactt
     4501 tggggattgt agggaatttc caggtctgaa gatcctgctg cttgctgtgc ttttttctta
     4561 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     4621 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     4681 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     4741 aacaggccag tattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     4801 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
     4861 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
     4921 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
     4981 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
     5041 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     5101 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     5161 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     5221 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     5281 tccccagtcc cgcccaggcc acgcctccca tggtaatagc gatgactaat acgtagatgt
     5341 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     5401 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     5461 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5521 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     5581 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     5641 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga cccaggtggc
     5701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5821 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5881 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5941 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6001 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6061 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6121 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6181 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6241 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6301 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6361 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6421 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6481 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6541 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6601 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6961 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7501 atgttctttc ctgcgttatc ccctgcttct gtggataacc gtattaccgc ctttgagtga
     7561 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7621 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7681 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7741 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7801 ctgcaataaa caagttagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     7861 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     7921 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     7981 taggggcggg actatggttg ctgactaatt gagatgcgga tccgctggca cgacaggttt
     8041 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8101 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8161 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg gctgcaggtc
     8221 gagggatctc cataagagaa gagggacagc tatgactggg agtagtcagg agaggaggaa
     8281 aaatctggct agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa aaaataacac
     8341 aaaacaaaat at
//
