LOCUS       sequence-488161-        7398 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_138, derived from human fusion protein PAX5_FOXP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_138
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(931..1641,1642..1644,1645..1647))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(973..994)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1322..1341
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1581..1602
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1644..1653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1729..1932)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1758..1778)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1933..2236
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2493..2537)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2722..2955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3315..3432
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3924..4049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4096..4353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4355..4734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4765..4869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4870..4938,4939..5730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5088..5107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5901..6489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6390..6409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6643..6660
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6728..6862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6752..6771)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6806..6825
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6868..7063)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6882..7017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7122..7143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7158..7163,7164..7181,7182..7188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7196..7212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7201..7223
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7220..7236
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7220..7236
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gccgacagta tgcatcgagg ttgttgtgat
      601 tactgacggc ccttgtgtta cgggagtaag tggtgccgta ggagtggttg gggtgtgggg
      661 caagctctgt ggactggctt cagaagcact cttgcttaag gtgacggaac tgactaagtt
      721 caacggttga ggagctgctt tgggttcggt agactttaca tgaaggtgcg tcatcatcgc
      781 ctgcaagcgt tctttgtctt tggctaattg taactcaagt tgctggacga cttgcatttg
      841 aacgcggcat tgcgcagtag aacggtcgtc aagagcatgt tcggagttcc gggaagcggc
      901 cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat
      961 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
     1021 gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
     1081 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
     1141 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
     1201 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
     1261 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
     1321 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     1381 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
     1441 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
     1501 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     1561 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
     1621 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg cccgcggtac
     1681 cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag ctctgcttat
     1741 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     1801 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     1861 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     1921 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     1981 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     2041 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     2101 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     2161 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     2221 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     2281 ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg
     2341 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     2401 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     2461 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     2521 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     2581 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     2641 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     2701 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     2761 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     2821 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     2881 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     2941 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     3001 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     3061 ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc catgttctaa
     3121 tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc ataatcccta
     3181 atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg tattactact
     3241 gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt tctgctgtcc
     3301 ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt
     3361 atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct
     3421 tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag atgttcagcc
     3481 tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc tactactcct
     3541 tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg ctgtgctttt
     3601 ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     3661 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     3721 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     3781 gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     3841 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     3901 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     3961 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     4021 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     4081 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     4141 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     4201 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     4261 ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct
     4321 cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg actaatacgt
     4381 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     4441 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     4501 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     4561 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     4621 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     4681 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcgaccca
     4741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4981 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5041 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5101 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5161 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5281 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5401 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5461 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6541 gctcacatgt tctttcctgc gttatcccct gcttctgtgg ataaccgtat taccgccttt
     6601 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6661 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6721 tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6781 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6841 tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     6901 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     6961 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     7021 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg ctggcacgac
     7081 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7141 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7201 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgggctg
     7261 caggtcgagg gatctccata agagaagagg gacagctatg actgggagta gtcaggagag
     7321 gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa
     7381 taacacaaaa caaaatat
//
