LOCUS       sequence-488162-        7707 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_140, derived from human fusion protein PAX5_JAK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7707)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_140
FEATURES             Location/Qualifiers
     source          1..7707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1240..1950,1951..1953,1954..1956))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1282..1303)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1631..1650
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1890..1911
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1953..1962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2038..2241)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2067..2087)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2242..2545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2802..2846)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3031..3264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3624..3741
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4233..4358
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4405..4662
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4664..5043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5074..5178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5179..5247,5248..6039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5397..5416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6210..6798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6699..6718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6952..6969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7037..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7061..7080)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7115..7134
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7177..7372)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7191..7326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7431..7452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7467..7472,7473..7490,7491..7497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7505..7521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7510..7532
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7529..7545
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7529..7545
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca cagcaactcg tagtcgggga ccatgcccgt
      601 cagcgtaggc gcgctatatg agccctgtcc agcgggcgga acatggggcg ggtatccggg
      661 caatgtggtt gatgctaaat cgcgccccgt tacaatagga tacgactgcg gccctggaac
      721 ggatgaccca atgtctgcgg gtgttggaga cgctaaattg gccttcatgt catccagtcc
      781 tccggccaag ctagccatcg cgctatactc agtcgtctgt tcaggtttaa ttggctcagt
      841 ggttgtaaaa atatcactgt agtgctggcg ttcgaaaacg cgatctaaga cttcaagctg
      901 ctgctgagta aacaggtcgc cgcgcatttg cttacgcagg aagtcgcgtc ccggcagaga
      961 gtgtccgttt ggcacggggc tttcctggat gccttcatcg cgtttacgct tgttagtatc
     1021 ggccgaaggt gaggtaattc ctaaaattcc agagatgcta taacttgacc cggcgctgtc
     1081 ggttgaaaca ctcgatactt gtgttaccga ccccgtagac acgatcgaat gggagctcgc
     1141 tggcacgggt tggttaggcg gctgttgcac tttagtgcga atgatgcgat taatcgaccg
     1201 ggaagcggcc gcacttcccc cggagcctcc gcttccgccc ttgtacagct cgtccatgcc
     1261 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
     1321 ggggtctttg ctcagcttgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg
     1381 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc
     1441 gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat
     1501 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
     1561 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     1621 ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta
     1681 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
     1741 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
     1801 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
     1861 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
     1921 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatcccgggc
     1981 ccgcggtacc gtcgactgca gaattcggtt cactaaacga gcccggttca ctaaacgagc
     2041 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     2101 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     2161 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     2221 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     2281 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     2341 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     2401 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     2461 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     2521 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     2581 cgtaattgat tactattaat aactagcacg cgttcgaagc ttgagctcgg tcccctcggg
     2641 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     2701 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     2761 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     2821 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     2881 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     2941 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     3001 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     3061 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     3121 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     3181 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     3241 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     3301 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     3361 aattcacttc tccaattgtc cctcatatct cctccttatg gtgttttact aaacttttcc
     3421 atgttctaat cctcatcctg tctacttgcc acacaatcat cacctgccat ctgttttcca
     3481 taatccctaa tgatctttgc ttttcttctt ggcactactt ttatgtcact attatcttgt
     3541 attactactg ccccttcacc tttccagagg agctttgctg gtcctttcca aagtggattt
     3601 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     3661 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     3721 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtactgc tgtcttaaga
     3781 tgttcagcct gatctcttac ctgtcctata attttcttta attctttatt catagattct
     3841 actactcctt gactttgggg attgtaggga atttccaggt ctgaagatcc tgctgcttgc
     3901 tgtgcttttt tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     3961 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     4021 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     4081 ccttctgatg tttctaacag gccagtatta actgcgaatc gttctagctc cctgcttgcc
     4141 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     4201 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     4261 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     4321 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     4381 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     4441 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     4501 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     4561 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     4621 ctgagggctc gccactcccc agtcccgccc aggccacgcc tcccatggta atagcgatga
     4681 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     4741 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     4801 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     4861 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     4921 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     4981 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     5041 gtcgacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7021 attcattaat gcagcggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     7081 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     7141 tgtaaccatt ataagctgca ataaacaagt tagctttttg caaaagccta ggcctccaaa
     7201 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     7261 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     7321 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcggatccgc
     7381 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7441 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7501 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7561 cttgggctgc aggtcgaggg atctccataa gagaagaggg acagctatga ctgggagtag
     7621 tcaggagagg aggaaaaatc tggctagtaa aacatgtaag gaaaatttta gggatgttaa
     7681 agaaaaaaat aacacaaaac aaaatat
//
