LOCUS       sequence-488163-        7845 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_141, derived from human fusion protein PAX5_NCOA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7845)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_141
FEATURES             Location/Qualifiers
     source          1..7845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1378..2088,2089..2091,2092..2094))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1420..1441)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1769..1788
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2028..2049
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2091..2100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2176..2379)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2205..2225)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2380..2683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2940..2984)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3169..3402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3762..3879
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4371..4496
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4543..4800
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4802..5181
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5212..5316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5317..5385,5386..6177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5535..5554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6348..6936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6837..6856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7090..7107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7175..7309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7199..7218)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7253..7272
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7315..7510)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7329..7464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7569..7590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7605..7610,7611..7628,7629..7635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7643..7659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7648..7670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7667..7683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7667..7683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca atagcgatca tacgagcctt cgcggcggta
      601 catgggatcg cgggagtcac gcgagtctcc aaagccgtag ggatcgcggc gtgtcggtga
      661 tgggcgtgaa ggggctgtat tcatattacg acgttcggcg gctttattaa tatctggcac
      721 cataccggtc agagtaggcg cgctatagga cccttgccct gccgggggga cgtgaggtgg
      781 gtaacctgga agtgtggtcg atgccagatc gcgacctgtc acaatggggt aggactgggg
      841 acctgggacc gaagacccaa tgtctgctgg tgtaggggag gccagattcg ccttcatgtc
      901 atccaacccc ccggccaaag aggccattgc tgagtactcc gttgtctgtt cgggcttgat
      961 gggttctgtt gtggtaaaaa tgtccgagta atgctggcgt tcaaaaacac ggtccagaac
     1021 ctctaattgc tgttgggtga acagatcacc gcgcatctgt ttacgcagaa agtcacgacc
     1081 cggtaaacta tggccatttg ggacagggct ctcttgaata ccttcgtcac gtttgcgctt
     1141 attcgtatca gcggatgggc tggtgattcc taaaatacca ctaatgctat aacttgagcc
     1201 agcactatcg gtcgaaaccg agctgacttg tgtgacagag cctgtcgaga cgatggagtg
     1261 ggaactggcg ggcacgggtt gattgggggg ctgctggact tttgtacgga tgatgcgatt
     1321 aatggaagac actgaccggg aagcggccgc acttcccccg gagcctccgc ttccgccctt
     1381 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     1441 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
     1501 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     1561 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     1621 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     1681 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     1741 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     1801 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     1861 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     1921 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     1981 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     2041 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc
     2101 gaccggtgga tcccgggccc gcggtaccgt cgactgcaga attcggttca ctaaacgagc
     2161 ccggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     2221 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     2281 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     2341 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     2401 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     2461 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     2521 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     2581 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     2641 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     2701 gctatgaact aatgaccccg taattgatta ctattaataa ctagcacgcg ttcgaagctt
     2761 gagctcggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     2821 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     2881 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
     2941 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
     3001 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
     3061 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
     3121 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
     3181 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
     3241 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
     3301 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
     3361 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
     3421 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     3481 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tccttatggt
     3541 gttttactaa acttttccat gttctaatcc tcatcctgtc tacttgccac acaatcatca
     3601 cctgccatct gttttccata atccctaatg atctttgctt ttcttcttgg cactactttt
     3661 atgtcactat tatcttgtat tactactgcc ccttcacctt tccagaggag ctttgctggt
     3721 cctttccaaa gtggatttct gctgtccctg taataaaccc gaaaattttg aatttttgta
     3781 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     3841 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     3901 tgtactgctg tcttaagatg ttcagcctga tctcttacct gtcctataat tttctttaat
     3961 tctttattca tagattctac tactccttga ctttggggat tgtagggaat ttccaggtct
     4021 gaagatcctg ctgcttgctg tgcttttttc ttacttttgt tttgctcttc ctctatcttg
     4081 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
     4141 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
     4201 cccagtattt gtctacagcc ttctgatgtt tctaacaggc cagtattaac tgcgaatcgt
     4261 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     4321 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     4381 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     4441 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     4501 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     4561 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     4621 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     4681 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca
     4741 gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc
     4801 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     4861 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     4921 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     4981 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5041 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     5101 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     5161 aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
     5221 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5281 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5341 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5401 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5461 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5521 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5581 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5641 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5701 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5761 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5821 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5881 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5941 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6001 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6061 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6121 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6181 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6361 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6421 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6481 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6541 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6601 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6661 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6721 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6781 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6841 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6901 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6961 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgct
     7021 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7081 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7141 ctccccgcgc gttggccgat tcattaatgc agcggatcca gacatgataa gatacattga
     7201 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     7261 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta gctttttgca
     7321 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     7381 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     7441 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     7501 attgagatgc ggatccgctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7561 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7621 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7681 gaccatgatt acgccaagct tgggctgcag gtcgagggat ctccataaga gaagagggac
     7741 agctatgact gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga
     7801 aaattttagg gatgttaaag aaaaaaataa cacaaaacaa aatat
//
