LOCUS       sequence-488164-        8145 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_143, derived from human fusion protein PAX5_NOL4L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8145)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_143
FEATURES             Location/Qualifiers
     source          1..8145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1678..2388,2389..2391,2392..2394))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1720..1741)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2069..2088
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2328..2349
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2391..2400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2476..2679)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2505..2525)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2680..2983
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3240..3284)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3469..3702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4062..4179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4671..4796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4843..5100
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5102..5481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5512..5616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5617..5685,5686..6477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5835..5854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6648..7236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7137..7156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7390..7407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7475..7609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7499..7518)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7553..7572
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7615..7810)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7629..7764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7869..7890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7905..7910,7911..7928,7929..7935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7943..7959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7948..7970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7967..7983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7967..7983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca caggcgttcg gggtccatac cctcagaatc
      601 gttcattttg tcattatctt catggtcatc atggtcatcg tcatcgtcgt cggccgttgg
      661 agcacgtcca acggtcaaat cttcggggca accggacact tcagtcttaa tgctgtcata
      721 gcttccgctc gaataagggg gcgactctgg agtagtcttt acgccgtatt tcacgcggga
      781 acgcaatccg tctgcgccac aaccgtccga agggtagctg gccgtcccca aagcagtcgc
      841 tgggggaagt ttgtcacaca gattaattgg ctgatcctcc gctgcagtag tgaaatccat
      901 aggtgccact gcgccgtttc cgttcatctc aggaaatgct ggatcaccct gcgtcgaaga
      961 agacgtggac ggattcaagg tacttgaacc attgcctgaa ccagactctg aggaggagtc
     1021 gtcgggaacc attcctgtca gagttggcgc agaataagat ccttggcccg caggtggcac
     1081 atgcggggga taccccggta atgtggtaga tgcaaggtca cgaccagtga caattgggta
     1141 ggactgggga ccaggaaccg aagagccaat atcagcggga gtggggcttg caaggttcgc
     1201 tttcatatcg tctaagcctc cagctaagga cgccatcgct gagtattccg tagtttgttc
     1261 aggcttgata ggttcggtgg tcgtgaagat gtcagagtag tgttgacgct caaatacgcg
     1321 gtccaagact tctaattgct gttgtgtgaa cagatctccg cgcatctgct tacgcaaaaa
     1381 gtcacgaccc ggaagactat gaccgtttgg cacaggactc tcttgaatac cttcatcacg
     1441 tttacgcttg ttggtgtcgg cggaaggcga ggtgatacct aagataccgg agatactgta
     1501 tgatgaacca gcggaatcag tggacacact acttacttgg gttactgacc ccgtgctaac
     1561 aatactgtga gatgaagcag gcactggttg attgggcggt tgctgaactt tagtacgaat
     1621 aatacgatta atggaccggg aagcggccgc acttcccccg gagcctccgc ttccgccctt
     1681 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     1741 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
     1801 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     1861 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     1921 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     1981 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     2041 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     2101 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     2161 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     2221 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     2281 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     2341 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc
     2401 gaccggtgga tcccgggccc gcggtaccgt cgactgcaga attcggttca ctaaacgagc
     2461 ccggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     2521 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     2581 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     2641 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     2701 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     2761 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     2821 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     2881 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     2941 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     3001 gctatgaact aatgaccccg taattgatta ctattaataa ctagcacgcg ttcgaagctt
     3061 gagctcggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
     3121 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
     3181 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
     3241 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
     3301 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
     3361 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
     3421 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
     3481 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
     3541 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
     3601 actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata
     3661 gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt
     3721 tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt
     3781 actactttat atttatataa ttcacttctc caattgtccc tcatatctcc tccttatggt
     3841 gttttactaa acttttccat gttctaatcc tcatcctgtc tacttgccac acaatcatca
     3901 cctgccatct gttttccata atccctaatg atctttgctt ttcttcttgg cactactttt
     3961 atgtcactat tatcttgtat tactactgcc ccttcacctt tccagaggag ctttgctggt
     4021 cctttccaaa gtggatttct gctgtccctg taataaaccc gaaaattttg aatttttgta
     4081 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     4141 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     4201 tgtactgctg tcttaagatg ttcagcctga tctcttacct gtcctataat tttctttaat
     4261 tctttattca tagattctac tactccttga ctttggggat tgtagggaat ttccaggtct
     4321 gaagatcctg ctgcttgctg tgcttttttc ttacttttgt tttgctcttc ctctatcttg
     4381 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
     4441 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
     4501 cccagtattt gtctacagcc ttctgatgtt tctaacaggc cagtattaac tgcgaatcgt
     4561 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     4621 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     4681 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     4741 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     4801 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     4861 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     4921 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     4981 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca
     5041 gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc
     5101 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     5161 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5221 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     5281 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5341 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     5401 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     5461 aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
     5521 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5581 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5641 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5701 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5761 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5821 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5881 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5941 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6001 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6061 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6121 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6181 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6241 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6301 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6361 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6421 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6481 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6541 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6601 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6661 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6721 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6781 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6841 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6901 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6961 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7021 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7081 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7141 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7201 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7261 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7321 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7381 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7441 ctccccgcgc gttggccgat tcattaatgc agcggatcca gacatgataa gatacattga
     7501 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     7561 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta gctttttgca
     7621 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     7681 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     7741 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     7801 attgagatgc ggatccgctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7861 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7921 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7981 gaccatgatt acgccaagct tgggctgcag gtcgagggat ctccataaga gaagagggac
     8041 agctatgact gggagtagtc aggagaggag gaaaaatctg gctagtaaaa catgtaagga
     8101 aaattttagg gatgttaaag aaaaaaataa cacaaaacaa aatat
//
