LOCUS       sequence-488198-        8550 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_178, derived from human fusion protein SMARCA2_ZNF384.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8550)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_178
FEATURES             Location/Qualifiers
     source          1..8550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(345..364)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    410..465
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(464..483)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(826..842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(826..842)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(839..861)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    850..866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(874..880,881..898,899..904))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    919..940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        999..1194
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1045..1180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1200..1334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1237..1256)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1291..1310
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1402..1419)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1573..2161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1653..1672)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2332..3123,3124..3192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2955..2974
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3193..3297)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        3328..3707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             3709..3966
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4013..4138
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4630..4747
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5107..5340
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5525..5569
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        5826..6129
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6130..6333
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6284..6304
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6409..6418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6415..6417,6418..6420,6421..7131)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6460..6481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6721..6740)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7068..7089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaaaaaaagc gaatgctgga atctggcttg ccggagatga atgatccgta cgttctgtcc
       61 ccggaggacg acgacgacca tcagaaagat ggctgaaccg gttctagata agattacggt
      121 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
      181 ggggggactg gaagggctaa ttcactccca aagaagacaa gatctgcttt ttgcctgtac
      241 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      301 actgcttaag cctcaataaa gcttcagctg ctcgagctag cagatctttt tccctctgcc
      361 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      421 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      481 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
      541 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
      601 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
      661 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
      721 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
      781 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
      841 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      901 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      961 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     1021 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     1081 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     1141 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     1201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1321 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1381 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1441 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1501 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1561 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1621 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1681 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1741 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1801 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1861 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1921 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1981 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2041 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2101 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2161 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2221 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2281 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2341 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2401 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2461 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2521 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2581 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2641 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2701 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2761 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2821 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2881 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2941 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3001 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3061 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3301 atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat
     3361 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3421 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3481 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3541 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3601 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3661 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggga ggcgtggcct
     3721 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     3781 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3841 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3901 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3961 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     4021 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     4081 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     4141 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     4201 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     4261 taatactggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     4321 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     4381 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     4441 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca ggatcttcag acctggaaat
     4501 tccctacaat ccccaaagtc aaggagtagt agaatctatg aataaagaat taaagaaaat
     4561 tataggacag gtaagagatc aggctgaaca tcttaagaca gcagtacaaa tggcagtatt
     4621 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     4681 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     4741 aaattttcgg gtttattaca gggacagcag aaatccactt tggaaaggac cagcaaagct
     4801 cctctggaaa ggtgaagggg cagtagtaat acaagataat agtgacataa aagtagtgcc
     4861 aagaagaaaa gcaaagatca ttagggatta tggaaaacag atggcaggtg atgattgtgt
     4921 ggcaagtaga caggatgagg attagaacat ggaaaagttt agtaaaacac cataaggagg
     4981 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     5041 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     5101 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     5161 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     5221 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     5281 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     5341 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     5401 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     5461 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     5521 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     5581 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     5641 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     5701 accattatcg tttcagaccc acctcccaac cccgagggga ccgagctcaa gcttcgaacg
     5761 cgtgctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5821 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5881 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5941 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6001 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6061 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6121 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     6181 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     6241 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     6301 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgggc tcgtttagtg
     6361 aaccgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     6421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     6481 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     6541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     6601 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     6661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     6721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     6781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     6841 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     6901 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     6961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     7021 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     7081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggaagc
     7141 ggaggctccg ggggaagtgc ggccgcttcc cggatgtcaa ctccaacaga tcccggagct
     7201 atgccgcacc cgggtccgtc tccaggtccg ggcccgagcc ccggaccgat cctgggtccg
     7261 tcgccgggcc cgggcccgtc tccgggtagc gtgcatagca tgatgggtcc gtcgccgggc
     7321 ccgccctcag tcagtcaccc gatgccaacc atgggttcca ctgattttcc gcaagaaggc
     7381 atgcatcaga tgcataagcc gatcgatggt atccacgata aaggtattgt agaagacatc
     7441 cactgcggca gcatgaaggg caccggcatg cgtccgccgc atccgggcat gggcccgcca
     7501 caaagcccga tggaccagca ttcccaaggt tatatgagcc cgcacccgag cccgctgggc
     7561 gcgccggagc atgtttccag cccgatgtct ggtggcggtc cgaccccgcc acaaatgccg
     7621 ccatcgcaac caggcgctct tatcccgggc gacccgcaag cgatgagcca accaaatcgt
     7681 ggtccgagcc cgttcagccc cgtgcagctc catcagttgc gtgcccagat tttggcgtac
     7741 aagatgctgg cgcgcggtca gccgctgccg gaaacactgc agctcgcggt tcagggcaaa
     7801 cgcaccctcc cgggtttgca gcaacaacag caacaacagc agcaacagca acaacaacag
     7861 cagcagcagc agcagcaaca gcaacagcct cagcagcagc cgccacaacc gcagacccag
     7921 caacaacaac agccggcact ggtcaattat aaccgtccta gcggccgtat ggaagagagc
     7981 cacttcaaca gcaaccccta cttctggccg agcattccga cggtgtccgg ccagatcgaa
     8041 aataccatgt tcattaacaa gatgaaggac cagttactgc ccgagaaagg ttgcggtctg
     8101 gcgccgccac actatccgac cctgctcact gtcccggcaa gtgtttccct gccgtccggc
     8161 atcagtatgg ataccgaatc gaagtctgat cagctgacac ctcacagcca agccagcgtc
     8221 acccagaaca ttaccgttgt tccggtgccg tcaactggcc tgatgaccgc gggtgtttct
     8281 tgtagccaac gttggcgtcg tgaagggagc caatccagag gtccgggttt agttatcacc
     8341 agcccgtctg gtagcttggt taccaccgct agctcagcgc aaaccttccc catcagcgct
     8401 ccgatgatcg tgtctgcact gccgccgggt agccaagcgc tacaggtggt gccggacctg
     8461 tctaagaagg tggccagcac gctgactgag gaaggtggag gtggcggtgg cggtggtggt
     8521 tccgtggcgc cgaaaccacc gcgtggtcgt
//
