LOCUS       sequence-488206-        8244 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_187, derived from human fusion protein TAF15_ZNF384.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8244)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_187
FEATURES             Location/Qualifiers
     source          1..8244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1777..2487,2488..2490,2491..2493))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1819..1840)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2168..2187
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2427..2448
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2490..2499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2575..2778)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2604..2624)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2779..3082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3339..3383)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3568..3801
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4161..4278
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4770..4895
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4942..5199
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5201..5580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5611..5715
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5716..5784,5785..6576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5934..5953)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6747..7335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7236..7255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7489..7506
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7574..7708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7598..7617)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7652..7671
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7714..7909)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7728..7863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7968..7989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8004..8009,8010..8027,8028..8034)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8042..8058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8047..8069
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8066..8082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8066..8082
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gccgtctttc tgatggtcat catcatcctc
      601 aggggagagg acataagggt cattcatctc gggcagccct gattccagca tccgcttctt
      661 cttccggccc cggggtggct taggagccac actgccacct ccaccaccac ctccgcctcc
      721 ttcctcggtt agggtcgatg ctaccttctt ggagaggtca gggacaacct gcagggcttg
      781 tgagccaggg ggaagagctg agacaatcat gggagccgaa atggggaagg tctgagctga
      841 tgatgctgtg gtcacaagag agcctgaggg ggacgtgatt accaaacccg gaccccttga
      901 ttgactccct tctcttctcc acctctgaga acaggagact ccagcagtca tcagtcctgt
      961 agacggcaca gggaccaccg tgatattctg ggtaacggac gcttggctgt gtggggtcag
     1021 ctggtctgac ttggactctg tgtccatact gatgcctgag ggcagggaca ctgaggcagg
     1081 cactgtcagc aaggtggggt agtgaggtgg ggccagacca cagcccttct ctggcaacag
     1141 ctgatccttc atcttgttga tgaacattgt gttctcgatc tgacctgaga ctgtggggat
     1201 agaaggccag aagtacgggt tagaattgaa gtgagattct tccattctac cttgtgtgtg
     1261 gtggctgtag ttttcccttt gtgaatgata ggactgctgg ttttgactat aggaatcatg
     1321 ctgctgatca taatttgact gctcatcata tgagccttga tgttgatcat agcctgactg
     1381 ctggtcatat gaatcttgtt gaccatagtc tggctggtca taggaaggtg ctcttccacc
     1441 ttggcttcct gatgattcca tgttttgctg ctgtccctgg ttattatatg gttgctggct
     1501 atatgagctc tgcttttgat tctcataacc accataggac tgtgaataac ctgactgact
     1561 ttgtccataa ctggagtaac cgctgtagtt ctgtccataa gaggaatcag tcgtttgccc
     1621 atagccagaa tagctttgtg atgcttgtcc atagccttgg ctgcctggat ttccataggt
     1681 agaataactt tgctgctcac ccccagactg accgtaactt ccagaatccg acatccggga
     1741 agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt ccatgccgag
     1801 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     1861 gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     1921 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     1981 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     2041 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     2101 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     2161 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     2221 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     2281 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     2341 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     2401 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     2461 cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat cccgggcccg
     2521 cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta aacgagctct
     2581 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     2641 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     2701 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     2761 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     2821 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     2881 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     2941 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     3001 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     3061 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     3121 aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc cctcggggtt
     3181 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     3241 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     3301 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     3361 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     3421 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     3481 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     3541 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     3601 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     3661 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     3721 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     3781 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     3841 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     3901 tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa cttttccatg
     3961 ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg ttttccataa
     4021 tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt atcttgtatt
     4081 actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag tggatttctg
     4141 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     4201 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     4261 cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt cttaagatgt
     4321 tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat agattctact
     4381 actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc tgcttgctgt
     4441 gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     4501 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     4561 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     4621 tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct gcttgcccat
     4681 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     4741 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     4801 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     4861 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     4921 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     4981 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     5041 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     5101 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     5161 agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata gcgatgacta
     5221 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5281 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5341 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5401 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     5461 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     5521 ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc
     5581 gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5641 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5701 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5761 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5821 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5881 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5941 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6001 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6061 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6121 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6181 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6241 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6301 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6361 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6421 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6481 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6541 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6601 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6661 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6721 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6901 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7321 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7381 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7441 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7501 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7561 cattaatgca gcggatccag acatgataag atacattgat gagtttggac aaaccacaac
     7621 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     7681 aaccattata agctgcaata aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa
     7741 gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat
     7801 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     7861 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgcg gatccgctgg
     7921 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7981 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     8041 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
     8101 gggctgcagg tcgagggatc tccataagag aagagggaca gctatgactg ggagtagtca
     8161 ggagaggagg aaaaatctgg ctagtaaaac atgtaaggaa aattttaggg atgttaaaga
     8221 aaaaaataac acaaaacaaa atat
//
