LOCUS       sequence-488210-        7311 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_191, derived from human fusion protein TCF3_PBX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7311)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_191
FEATURES             Location/Qualifiers
     source          1..7311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(844..1554,1555..1557,1558..1560))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(886..907)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1235..1254
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1494..1515
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1557..1566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1642..1845)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1671..1691)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1846..2149
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2406..2450)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2635..2868
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3228..3345
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3837..3962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4009..4266
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4268..4647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4678..4782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4783..4851,4852..5643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5001..5020)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5814..6402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6303..6322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6556..6573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6641..6775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6665..6684)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6719..6738
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6781..6976)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6795..6930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7035..7056
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7071..7076,7077..7094,7095..7101)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7109..7125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7114..7136
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7133..7149
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7133..7149
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca attactggtg tcactatgaa cacttccggg
      601 cccttcagtc ggactcgtga cggatgacgg cgttgtagca tcctgccatc ccccatttgc
      661 tgagatccct tgtggggaat acatttgact ggcagctaac ccatcgctat atccacccgt
      721 ctgagaaatg acgtgacgta acgtgtcaac ttgagactgt acattggcac ctacttgagc
      781 tccctggtag gagtccccgt tccgggaagc ggccgcactt cccccggagc ctccgcttcc
      841 gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      901 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
      961 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     1021 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     1081 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     1141 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     1201 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     1261 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     1321 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     1381 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     1441 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     1501 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     1561 ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc ggttcactaa
     1621 acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     1681 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     1741 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     1801 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     1861 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     1921 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     1981 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     2041 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     2101 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     2161 atatgggcta tgaactaatg accccgtaat tgattactat taataactag cacgcgttcg
     2221 aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     2281 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     2341 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     2401 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     2461 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     2521 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     2581 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     2641 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     2701 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     2761 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     2821 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     2881 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     2941 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     3001 tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact tgccacacaa
     3061 tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct tcttggcact
     3121 acttttatgt cactattatc ttgtattact actgcccctt cacctttcca gaggagcttt
     3181 gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa attttgaatt
     3241 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     3301 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
     3361 gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc tataattttc
     3421 tttaattctt tattcataga ttctactact ccttgacttt ggggattgta gggaatttcc
     3481 aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg ctcttcctct
     3541 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     3601 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     3661 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt attaactgcg
     3721 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     3781 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     3841 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     3901 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     3961 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     4021 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     4081 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     4141 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa
     4201 aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca
     4261 cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     4321 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     4381 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     4441 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     4501 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     4561 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     4621 attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc
     4681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4861 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4921 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4981 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5041 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5101 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5161 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5221 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5281 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5341 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5401 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5461 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5521 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5581 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5641 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5701 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5941 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6601 ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg gatccagaca tgataagata
     6661 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6721 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttagctt
     6781 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6841 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     6901 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     6961 tgactaattg agatgcggat ccgctggcac gacaggtttc ccgactggaa agcgggcagt
     7021 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7081 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7141 agctatgacc atgattacgc caagcttggg ctgcaggtcg agggatctcc ataagagaag
     7201 agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta gtaaaacatg
     7261 taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata t
//
