LOCUS       sequence-488263-        8589 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Express mEGFP-tagged fusion protein ARID1B_ZNF384 with an IDR 
            deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8589)
  TITLE     Kriwacki lab plasmids 2025
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_mEGFP_ARID1B_ZNF384-Delta-1-260
FEATURES             Location/Qualifiers
     source          1..8589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(2122..2832,2833..2835,2836..2838))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2164..2185)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2513..2532
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2772..2793
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2835..2844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2920..3123)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2949..2969)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3124..3427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(3684..3728)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3913..4146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4506..4623
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5115..5240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5287..5544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        5546..5925
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5956..6060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6061..6129,6130..6921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6279..6298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7092..7680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7581..7600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7834..7851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7919..8053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7943..7962)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7997..8016
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(8059..8254)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8073..8208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    8313..8334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8349..8354,8355..8372,8373..8379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8387..8403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8392..8414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8411..8427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8411..8427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca cgagctagcc aggtgttcaa cctggatggt
      601 gcttgtggtc acggtcagac agatgtcttt atgatgttcg gcagttttgt acggagtcag
      661 atcgaagcta cattgaggcg gtgggttcgg attgctgtcg ccgccaccac cgccctgtgg
      721 agcggcgccc ggagactgga agtgcggcgg cggttgctgc tgctgctgct gttgttgctg
      781 ttgctgctgc tgttgttgtt ggctcgcctg gctcgcctgc gcttgagctt gagcctgggc
      841 ctgcgcctgc gcctgcgcct gcgcttgcgc ctgcgcctgt gctacagcag ccgccgccgc
      901 cgcagcttgg acttgttgtt gcaggtctgg cgggttgtgc ttacgcatgt gtttcatcag
      961 atatgtctcg ctggtatatg cgcgtgagca gatagtgcac gtgtacacct tagcatgctt
     1021 caccgtatgg gtgctaagat ggacctctaa gctggctgca tcggtatacg cgcggtgaca
     1081 attatggcat ttaaacggtt tgtccttgtt gtgctgacga cggtggctct gcaggttgga
     1141 cagttgggtg aatgccttct cgcagcccgg atgtgcgcac ttatacggac gatcaccggt
     1201 atggatacgc gtgtgttgct gcaggtgtga cagctggcgg aagctttttt cgcaaaaatt
     1261 acagctgtag ggtttcgcgc cggagtggat acgaatgtgc tgcgcgagat atgaggagtt
     1321 tgcgaaggtt ttcgagcagt gcgggcactt gtgcggtttc gtttccgtgt gggacttgga
     1381 atgaatctgc atctcggact ttgaataaaa agtcaagctg cacatacggc agcggtacgt
     1441 tttgccgtct ttctgatggt cgtcgtcatc ttccgggctc aggacgtacg gatcattcat
     1501 ctcaggcaaa ccgctttcca gcatacgctt ctttttgcgt ccacgcggtg gtttcggggc
     1561 cacgctgcca ccaccaccgc cgccgccacc gccctcctca gtcaaggtgc tcgcaacttt
     1621 cttactcaga tcggggacta cctgcagcgc ctggctgcca ggcggcagcg cagaaacaat
     1681 cattggcgcg gagatcggga aggtttgcgc agacgatgcg gtggtcacga gggagccgct
     1741 cgggctggtg ataaccaaac ccggacctct gctctgacta ccttcgcgac gccaacgttg
     1801 gctgcagcta acacccgccg tcatcaagcc ggtagacggc acgggaacca cggtaatgtt
     1861 ctgggtcacg gacgcctgac tgtgcggggt cagttgatca ctcttgctct cggtatccat
     1921 agagatacct gacggcaagc tcacggaagc cggcaccgta agtaaggtcg ggtaatgtgg
     1981 cggagccaaa ccgcaaccct tctccggcag cagctggtct ttcatcttgt taataaacat
     2041 ggtattttcg atttgaccac taacggtcgg aatgctcggc cgggaagcgg ccgcacttcc
     2101 cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     2161 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
     2221 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     2281 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     2341 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     2401 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     2461 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     2521 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     2581 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     2641 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     2701 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     2761 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     2821 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
     2881 cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta tatagacctc
     2941 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     3001 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     3061 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     3121 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     3181 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     3241 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     3301 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     3361 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     3421 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     3481 ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag gtgggtctga
     3541 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     3601 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     3661 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     3721 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     3781 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     3841 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     3901 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     3961 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     4021 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     4081 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     4141 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     4201 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     4261 tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta atcctcatcc
     4321 tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct aatgatcttt
     4381 gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac tgccccttca
     4441 cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc cctgtaataa
     4501 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     4561 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     4621 aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc ctgatctctt
     4681 acctgtccta taattttctt taattcttta ttcatagatt ctactactcc ttgactttgg
     4741 ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt tttcttactt
     4801 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     4861 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     4921 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     4981 aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     5041 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     5101 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     5161 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     5221 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     5281 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     5341 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     5401 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     5461 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
     5521 ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg tagatgtact
     5581 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     5641 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     5701 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     5761 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5821 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     5881 accccgtaat tgattactat taataactag tcaataatca atgtcgaccc aggtggcact
     5941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     6001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     6061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     6121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     6181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     6241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     6301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6601 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6661 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6721 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6781 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6901 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     7021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7141 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7741 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7801 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7861 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcgga
     7921 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7981 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     8041 caataaacaa gttagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     8101 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     8161 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     8221 gggcgggact atggttgctg actaattgag atgcggatcc gctggcacga caggtttccc
     8281 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8341 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8401 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgggct gcaggtcgag
     8461 ggatctccat aagagaagag ggacagctat gactgggagt agtcaggaga ggaggaaaaa
     8521 tctggctagt aaaacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa
     8581 acaaaatat
//
