LOCUS       sequence-488269-       10899 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  CRE recombinase with miRNA-124 target sequence to repress transgene 
            expression in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Deglon Lab
  TITLE     Deglon Lab Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SIN-cPPT-G1B3-nlsCre-WPRE-miR124T
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3344..3368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3457..3477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3478..4506
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     protein_bind    complement(4592..4616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4639..4655)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4640..4656)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4669..5257
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4722..4742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5140..5151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5461..5694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5719..5737)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5720..5737)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6027..6046)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6260..6279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6379..6401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6439..6457)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6525..6629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6630..6698,6699..7490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7661..8249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8150..8169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8403..8420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8490..8510)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8495..8824
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8675..8810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9958..10023
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10153..10173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10598..10732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10635..10654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10689..10708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatccg atgtacgggc
     2161 cagatatacg cgttgacatt gattattgac taggatccgg taccgaattc gagctcggta
     2221 ccagatctaa catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc
     2281 ctgagctggc tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac
     2341 ctggcagcat tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca
     2401 ccttggcaca gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc
     2461 ccccctcaaa tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct
     2521 gacagcctgg catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt
     2581 ggcgccaccg gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag
     2641 gggatgccca ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca
     2701 gaagtccaag gacacaaatg ggtgagggga gagctctccc catagctggg ctgcggccca
     2761 accccacccc ctcaggctat gccagggggt gttgccaggg gcacccgatc ttcctcttca
     2821 tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc
     2881 ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga gcaggcccga
     2941 tcttcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
     3001 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
     3061 gagcaggccc gatcttcctc ttcatgccca gtgaatgact caccttggca cagacacaat
     3121 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
     3181 gagaagccca ttgagcaggc ccgggcatcg ccagtctagc ccactccttc ataaagccct
     3241 cgcatcccag gagcgagcag agccagagca ggttggagag gagacgcatc acctccgctg
     3301 ctcgcgggga tcctagtgga tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa
     3361 agcaggctcc accatgggaa ccaattcagt cgactggatc cggtaccgaa ttccagcaca
     3421 ctggcggccg ttactagtgg atctcccggg accatgccca agaagaagag gaaggtgtcc
     3481 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
     3541 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
     3601 aaaatgcttc agtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
     3661 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
     3721 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc
     3781 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
     3841 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
     3901 gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca
     3961 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
     4021 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
     4081 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
     4141 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
     4201 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
     4261 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga
     4321 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct
     4381 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg
     4441 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc
     4501 gattagccat taacgcgtaa atgattgcta taattatttg atatttatgg tgacatacga
     4561 gggaattcgc ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggtgata
     4621 attcgatatc aagcttatcg ataccgtcga cctcgaggga attccgataa tcaacctctg
     4681 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4741 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4801 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4861 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4921 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4981 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5041 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5101 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5161 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5221 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattcga gctcggtacc
     5281 taatggcatt caccgcgtgc cttaattcga atggcattca ccgcgtgcct taaacgcgtt
     5341 ggcattcacc gcgtgcctta aatgcattgg cattcaccgc gtgccttaag gtacctttaa
     5401 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5461 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5521 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5581 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5641 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     5701 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     5761 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     5821 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     5881 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     5941 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     6001 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     6061 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     6121 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     6181 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     6241 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     6301 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6361 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6421 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6481 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6841 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7801 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8401 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8461 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8641 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8761 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8821 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8881 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8941 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     9001 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     9061 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     9121 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     9181 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     9241 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     9301 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     9361 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     9421 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9481 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9541 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9601 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9661 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9721 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9781 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9841 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9901 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9961 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    10021 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    10081 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    10141 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    10201 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    10261 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
    10321 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
    10381 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
    10441 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10501 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10561 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10621 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10681 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10741 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10801 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10861 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
