LOCUS       sequence-488273-       11985 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Fluorescent reporters ECFP and Mito-Timer, with miRNA-124 target 
            sequence to repress transgene expression in neurons .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Deglon Lab
  TITLE     Deglon Lab Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SIN-cPPT-G1B3-CFP-IRES2-mito-timer-WPRE-miR124T
FEATURES             Location/Qualifiers
     source          1..11985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3344..3368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3411..3435
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3441..3443,3444..3446,3447..4160)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3486..3507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3747..3766)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4094..4115
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4177..4201)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4252..4276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4304..4878,4879..4881,4882..4890)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4471..4488)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4698..4717
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4891..4965
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      4993..5002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4999..5676
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5179..5199)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5591..5614
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5678..5702)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5725..5741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5726..5742)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5755..6343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5808..5828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6226..6237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6547..6780
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6805..6823)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6806..6823)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7113..7132)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7346..7365)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7465..7487
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7525..7543)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7611..7715
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7716..7784,7785..8576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8747..9335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9236..9255
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9489..9506
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9576..9596)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9581..9910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9761..9896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11044..11109
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11239..11259
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11684..11818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11721..11740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11775..11794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatccg atgtacgggc
     2161 cagatatacg cgttgacatt gattattgac taggatccgg taccgaattc gagctcggta
     2221 ccagatctaa catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc
     2281 ctgagctggc tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac
     2341 ctggcagcat tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca
     2401 ccttggcaca gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc
     2461 ccccctcaaa tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct
     2521 gacagcctgg catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt
     2581 ggcgccaccg gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag
     2641 gggatgccca ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca
     2701 gaagtccaag gacacaaatg ggtgagggga gagctctccc catagctggg ctgcggccca
     2761 accccacccc ctcaggctat gccagggggt gttgccaggg gcacccgatc ttcctcttca
     2821 tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc
     2881 ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga gcaggcccga
     2941 tcttcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
     3001 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
     3061 gagcaggccc gatcttcctc ttcatgccca gtgaatgact caccttggca cagacacaat
     3121 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
     3181 gagaagccca ttgagcaggc ccgggcatcg ccagtctagc ccactccttc ataaagccct
     3241 cgcatcccag gagcgagcag agccagagca ggttggagag gagacgcatc acctccgctg
     3301 ctcgcgggga tcctagtgga tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa
     3361 agcaggcttc gtttaaacac tagtggatcc cccgggctgc aggaattatc acaagtttgt
     3421 acaaaaaagc aggctccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3481 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     3541 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3601 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
     3661 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     3721 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     3781 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     3841 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
     3901 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     3961 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4021 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     4081 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4141 tggacgagct gtacaagtaa aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
     4201 tgattcgagg gaattcgcgg ccgcactcga gatggaagat cttccatcta gacccagctt
     4261 tcttgtacaa agtggtgata attcgatatc aagcttatcg atgcccctct ccctcccccc
     4321 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4381 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4441 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4501 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4561 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4621 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4681 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     4741 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     4801 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4861 gaaaaacacg atgataatat ggccacaacc atgtccgtcc tgacgccgct gctgctgcgg
     4921 ggcttgacag gctcggcccg gcggctccca gtgccgcgcg ccaagatcca ttcgttgggg
     4981 gatccaccgg tcgccaccat ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc
     5041 aaggtgcgca tggagggcac cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     5101 ggccgcccct acgagggcca caacaccgtg aagctgaagg tgaccaaggg cggccccctg
     5161 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
     5221 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
     5281 cgcgtgatga acttcgagga cggcggcgtg gcgaccgtga cccaggactc ctccctgcag
     5341 gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc cgacggcccc
     5401 gtgatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     5461 ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg ccactacctg
     5521 gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg ctactactac
     5581 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggagcagtac
     5641 gagcgcaccg agggccgcca ccacctgttc ctgtaggacc cagctttctt gtacaaagtg
     5701 gtgataattc gatatcaagc ttatcgatac cgtcgacctc gagggaattc cgataatcaa
     5761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6121 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6301 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcgagctc
     6361 ggtacctaat ggcattcacc gcgtgcctta attcgaatgg cattcaccgc gtgccttaaa
     6421 cgcgttggca ttcaccgcgt gccttaaatg cattggcatt caccgcgtgc cttaaggtac
     6481 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6541 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6601 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6661 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6721 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6781 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
     6841 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
     6901 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
     6961 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
     7021 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
     7081 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     7141 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     7201 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     7261 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     7321 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     7381 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7441 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     7501 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     7561 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7621 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7681 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7741 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7801 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7861 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7921 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7981 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8041 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8101 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8161 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8221 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8281 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8341 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8401 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8461 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8521 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8581 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8641 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8701 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8761 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8821 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8881 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8941 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9001 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9061 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9121 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9181 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9241 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9301 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9361 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9421 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9481 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9541 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     9601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9661 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9721 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9781 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9841 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9901 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     9961 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
    10021 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
    10081 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
    10141 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
    10201 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
    10261 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
    10321 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
    10381 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
    10441 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
    10501 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
    10561 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
    10621 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
    10681 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
    10741 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
    10801 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
    10861 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
    10921 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
    10981 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    11041 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    11101 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
    11161 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
    11221 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
    11281 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
    11341 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
    11401 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
    11461 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
    11521 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
    11581 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
    11641 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
    11701 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    11761 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    11821 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    11881 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    11941 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
