LOCUS       sequence-488291-        5521 bp    DNA     circular SYN 02-OCT-2025
DEFINITION  Distribution and localization of rat Rab3a Q81L mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5521)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: emiRFP 670 Rab3a Q81L
FEATURES             Location/Qualifiers
     source          1..5521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      548..557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2321..2442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2358..2377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2412..2431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2449..2904)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2586..2607)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2798..2817
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2931..3035
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3060..3080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3104..3314
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3126..3322
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3173..3308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3357..4151
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3357..4151
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3411..3430)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4021..4040
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4339..4358)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4383..4430
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4759..5347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5248..5267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggttcgcc accatggcgg aaggctccgt cgccaggcag cctgacctct tgacctgcga
      601 acatgaagag atccacctcg ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag
      661 cgaacatgat catcgcgtca tccaggccag cgccaacgcc gcggaatttc tgaatctcgg
      721 aagcgtactc ggcgttccgc tcgccgagat cgacggcgat ctgttgatca agatcctgcc
      781 gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc
      841 ctctacggag tactgcggtc tgatgcatcg gcctccggaa ggcgggctga tcatcgaact
      901 cgaacgtgcc ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc tggagcggat
      961 ccgcacggcg ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg
     1021 caccggctac gaccgggtga tggtgtatcg tttcgatgag caaggccacg gcctggtatt
     1081 ctccgagtgc catgtgcctg ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgac
     1141 tgtcccgcag atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc tggtcgacgt
     1201 cacctatcag ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga
     1261 catgtcgggc tgcttcctgc gctcgatgtc gccgtgccat ctgcagttcc tgaaggacat
     1321 gggcgtgcgc gccaccctgg cggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt
     1381 tgtctgtcac cattatctgc cgcgcttcat ccgtttcgag ctgcgggcga tctgcaaacg
     1441 gctcgccgaa aggatcgcga cgcggatcac cgcgcttgag agctccggac tcagatctcg
     1501 agctcaagct tcgaattctg cagtcgacat ggcctcagcc acagactctc gatatgggca
     1561 gaaggagtcc tcagaccaga acttcgacta tatgttcaag atcctgatca ttggtaacag
     1621 cagtgtgggc aaaacctcat tccttttccg ctacgcagac gactctttta ctccagcctt
     1681 tgtcagcact gtgggcatag acttcaaggt caaaaccatc taccgcaatg acaagaggat
     1741 caagctgcag atctgggaca cagcaggact ggagcggtac cgaaccatca ccacagccta
     1801 ctaccggggc gccatgggct tcattctaat gtatgacatc accaatgagg agtccttcaa
     1861 tgcagtgcag gactggtcca ctcagatcaa aacttactca tgggacaatg cccaggtgct
     1921 gctggtgggg aacaagtgcg acatggagga cgagcgagtg gtgtcctcag aacgaggccg
     1981 gcagctggcc gaccacctgg gctttgagtt ctttgaggcc agcgccaagg acaacattaa
     2041 tgtcaagcag acctttgaac gtctggtgga cgtgatctgt gagaagatgt cggagtccct
     2101 agatactgca gaccctgcag tcacgggtgc caagcagggc ccacagctca ccgaccagca
     2161 ggcaccacct catcaggatt gcgcctgctg aggatccacc ggatctagat aactgatcat
     2221 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2281 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2341 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2401 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     2461 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2521 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     2581 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2641 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     2701 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2761 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     2821 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     2881 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3001 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     3061 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3121 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3241 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3301 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     3361 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3421 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3481 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3541 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3601 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3661 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3721 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3781 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3841 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3901 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3961 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4021 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4081 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4141 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4201 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4261 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4321 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     4381 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     4441 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4501 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4561 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4621 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4681 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4741 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4801 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4861 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4921 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4981 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5041 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5101 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5161 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5221 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5281 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5341 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5401 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     5461 ttagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     5521 g
//
