LOCUS       sequence-488319-        8683 bp    DNA     circular SYN 03-OCT-2025
DEFINITION  Proximity labeling studies for CAPS1 dysfunction and secretory 
            trafficking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8683)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPS1 G476E Flag Apex2
FEATURES             Location/Qualifiers
     source          1..8683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..600
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4516..4539
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5411..5532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5448..5467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5502..5521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5539..5994)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5676..5697)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5888..5907
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6021..6125
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6127..6484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6127..6318
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6150..6170)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6335..6470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6519..7313
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6519..7313
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6573..6592)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7183..7202
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7501..7520)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7545..7592
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7921..8509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8410..8429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttggt accgggcccc ccctcgaggt cgacggtatc
      601 gccaccatgc tggacccttc gtccagcgaa gaggaatcgg atgagatcct ggaagaggag
      661 agcggcaagg aggtgctggg ctcggccgcg tccggagcgc gcctgtctcc aagccgcacc
      721 aacgagggct cggcgggcag cgccggcatg gggggcagtg gcgccggggc cggggtgggc
      781 gccggcggcg gtgggggtag cggcgcgagc agcggcggcg gggccggggg gctgcagccc
      841 agcagccgcg ccggcggcgg ccggccctcc agccccagcc cgtcggtggt gagcgagaag
      901 gagaaggaag agttggagcg gctgcagaag gaagaggagg agaggaagaa gaggctgcag
      961 ctgtacgtgt tcgtgatgcg ctgcattgcc taccccttca acgccaagca gcccaccgac
     1021 atggctcggc ggcagcagaa gatcagcaaa cagcagctgc agactgtcaa ggaccgattc
     1081 caggctttcc tcaatgggga gacccaaatc gtggcagatg aagcctttat gaatgctgtc
     1141 cagagctact atgaggtgtt tctcaagagt gaccgtgtgg cccgcatggt acagagtggg
     1201 ggctgctcag ccaacgactc tcgggaggtc ttcaagaaac acatcgagaa gagggtacgc
     1261 agcctacctg agattgatgg cctcagcaag gagactgtgc taagctcctg gatggctaag
     1321 tttgacgcca tctaccgtgg tgaggaggac cccaggaagc agcaagcccg gatgacagcc
     1381 agtgcagctt ctgagttgat tctgagcaag gaacagctct atgagatgtt ccagaacatt
     1441 cttgggatca agaaatttga acatcaactc ctatataatg cgtgtcagct ggacaatcca
     1501 gatgagcagg cagcccagat cagacgagag ctggacggac gtctccaaat ggcagaccaa
     1561 atagccaggg aacgcaaatt tcctaagttc gtatccaaag aaatggaaaa tatgtacatc
     1621 gaggagctga agtcctccgt caacctactc atggccaact tggagagcat gccggtgtcc
     1681 aagggtggag agttcaagct gcagaaactg aaacgcagtc acaacgcgtc catcattgac
     1741 atgggtgagg agagcgagaa ccagctgtcc aagtcggatg tcctgctgtc cttctccttg
     1801 gaggttgtca tcatggaagt ccaaggcctt aagtccttgg ctccaaaccg aatcgtgtac
     1861 tgcacaatgg aggtggaggg tggagagaaa cttcagactg atcaagcaga ggcttctaaa
     1921 ccaacctggg gcactcaagg cgacttctct acaacccacg cgctgccagc tgttaaagta
     1981 aagctattca cagagagcac tggtgtcctg gccttggaag acaaggaact cgagcgggtt
     2041 atcctccatc ccaccccaaa cagccccaaa caatccgagt ggcacaaaat gacagtctcc
     2101 aaaaactgcc ccgatcaaga cctcaaaatc aaactcgctg tccgaatgga taagcctcaa
     2161 aacatgaagc actctgggta cttatggacc attggtaaga atgtgtggaa gaggtggaag
     2221 aaaaggttct ttgtgttggt gcaggtcagc cagtacactt ttgccatgtg cagctatcga
     2281 gaaaagaaag cagaacctca ggaactccta cagctggatg gctacaccgt ggattacacc
     2341 gaccctcagc caggtttgga gggtggccga gccttcttca atgcagtcaa agaaggagac
     2401 acggtgatat ttgccagtga cgatgagcaa gatcgaatcc tgtgggtcca agctatgtac
     2461 agggccaccg ggcagtcgca caaacctgtg ccccctaccc aggtgcagaa gctcaacgcc
     2521 aagggcggaa acgtgcctca gctggatgcc cccatctccc agttttacgc agatagagct
     2581 caaaagcatg gcatggatga atttatctcg tccaacccct gtaactttga ccacgcttcc
     2641 ctctttgaga tggtacagcg gctgactttg gatcacagac taaacgattc ctattcttgc
     2701 ctgggctggt tcagtcctgg ccaggtgttt gtgttagacg agtactgcgc tcggaatgga
     2761 gtccgaggat gtcatcggca tctctgttac cttagagact tgctggaacg ggcagaaaat
     2821 ggcgccatga tcgaccccac gcttcttcat tacagctttg ccttctgcgc atcccatgtc
     2881 cacgggaaca ggcctgatgg aatcggaact gtgactgttg aagaaaaaga gcgctttgaa
     2941 gaaatcaaag agagactccg agttctgctg gaaaatcaaa ttacacattt caggtactgc
     3001 tttccgtttg gtcgacctga aggtgctttg aaagcgactc tctcactttt ggaacgggtt
     3061 ttgatgaaag atattgtcac tccagtaccc caagaggagg tcaaaactgt catccgtaaa
     3121 tgcctagagc aggctgctct agtcaactac tctcgcctat ctgagtatgc caaaatcgaa
     3181 gaaaatgtag gccgcttaat cactcccgcc aaaaagctcg aagacacaat ccgtcttgct
     3241 gaactagtca ttgaagtgct gcaacaaaac gaagagcacc acgccgaggc ctttgcgtgg
     3301 tggtcagact taatggtgga gcacgcggag acgttcctgt cactctttgc agtggacatg
     3361 gatgcagcat tagaggtgca acccccagac acgtgggaca gtttcccact cttccagctg
     3421 ctgaatgact ttctccgtac agactataac ttgtgcaatg gaaagtttca caaacacctg
     3481 caggatctgt tcgccccact cgttgttaga tacgtggatc tgatggagtc ctcaattgca
     3541 caatctatcc acaggggctt tgagcgggag tcatgggaac cagtcaataa cggatcgggt
     3601 acatcagaag atttgttctg gaaacttgat gcccttcaga ctttcatccg tgacctacac
     3661 tggcctgagg aagagtttgg aaagcactta gagcagcggc taaaactaat ggcaagcgac
     3721 atgattgagt cctgcgtcaa gagaaccagg attgcatttg aagttaagct gcaaaaaacc
     3781 agtcgatcaa cagattttcg agtcccacag tcaatatgca ccatgtttaa tgttatggtt
     3841 gatgccaaag ctcaatcaac aaaactttgc agcatggaaa tgggccagga gcatcaatac
     3901 cattcaaaaa tagacgaact aattgaagaa accgttaagg aaatgatcac actcttggtg
     3961 gcaaagtttg taactatatt ggaaggcgta ctggcaaagt tatccagata cgacgagggg
     4021 actttgtttt cttcgtttct gtcatttacg gtgaaggcgg catctaagta tgtggacgta
     4081 cctaaacccg ggatggatgt ggccgacgcc tacgtgactt tcgtccgcca ctctcaggat
     4141 gtccttcgtg ataaggtcaa tgaggagatg tatatagaaa ggttatttga tcaatggtac
     4201 aacagctcca tgaacgtcat ctgcacctgg ctgaccgacc ggatggacct ccagcttcac
     4261 atttatcagc tgaaaacact aattaggatg gtaaagaaaa cctacagaga tttccgattg
     4321 caaggggtcc tggattccac cttaaacagc aagacctatg agaccattcg aaatcggctc
     4381 actgtagagg aagctaccgc gtccgtgagt gagggagggg gcttgcaagg catcagcatg
     4441 aaggacagcg atgaagagga cgaggaggat gacggtggag gaggttctgg ccaccacact
     4501 ggactagtgg atccggacta caaggatgac gacgataagg ggaaatcata cccaacagtg
     4561 tccgcagact accaggatgc cgtggagaaa gccaagaaga agctgagagg gtttatcgca
     4621 gaaaagaggt gcgcacctct gatgctgaga ctggctttcc acagcgcagg cacctttgac
     4681 aagggaacca aaacaggcgg accctttgga acaatcaagc accctgctga actggcacat
     4741 agtgccaaca atgggctgga catcgccgtg cggctgctgg aacctctgaa agcagagttc
     4801 ccaattctgt cctacgccga tttttatcag ctggcaggag tggtcgctgt ggaggtcact
     4861 gggggcccca aggtgccttt ccacccagga cgggaggaca aaccagaacc acctccagag
     4921 gggcgcctgc cagatccgac aaagggctcc gaccatctgc gagatgtgtt tgggaaagct
     4981 atgggcctga ctgaccagga tatcgtcgca ctgtctggag ggcacaccat tggcgccgct
     5041 cataaggaaa ggtcaggctt cgagggaccc tggacaagca accccctgat tttcgacaat
     5101 tcttacttta ctgaactgct gagtggagag aaggaagggc tgctgcagct gcccagcgac
     5161 aaagccctgc tgtccgatcc cgtgttcaga cctctggtcg ataagtatgc agccgacgag
     5221 gatgcttttt tcgcagatta cgcagaagca catcagaagc tgtcagaact gggatttgcc
     5281 gacgcctaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     5341 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     5401 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5521 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     5581 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     5641 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     5701 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     5761 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     5821 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     5881 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     5941 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     6001 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6061 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6121 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6241 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6301 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6361 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6421 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6481 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6541 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6601 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6661 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     6721 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6781 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6841 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6901 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6961 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7021 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     7081 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7141 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7201 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     7261 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     7321 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7381 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7441 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     7501 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     7561 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     7621 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     7681 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     7741 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     7801 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7921 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7981 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8041 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8101 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8161 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8221 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8281 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8341 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8401 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8461 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8521 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8581 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     8641 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
