LOCUS       sequence-488321-        8653 bp    DNA     circular SYN 03-OCT-2025
DEFINITION  Bipolar rat CAPS1 R944L mutant used for proximity labeling studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8653)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPS1 R944L Flag Apex2
FEATURES             Location/Qualifiers
     source          1..8653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     554..570
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     555..571
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4486..4509
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5381..5502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5418..5437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5472..5491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5509..5964)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5646..5667)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5858..5877
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5991..6095
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6097..6454
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6097..6288
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6120..6140)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6305..6440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6489..7283
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6489..7283
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6543..6562)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7153..7172
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7471..7490)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7515..7562
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7891..8479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8380..8399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgaggt cgacggtatc gccaccatgc tggacccttc gtccagcgaa
      601 gaggaatcgg atgagatcct ggaagaggag agcggcaagg aggtgctggg ctcggccgcg
      661 tccggagcgc gcctgtctcc aagccgcacc aacgagggct cggcgggcag cgccggcatg
      721 gggggcagtg gcgccggggc cggggtgggc gccggcggcg gtgggggtag cggcgcgagc
      781 agcggcggcg gggccggggg gctgcagccc agcagccgcg ccggcggcgg ccggccctcc
      841 agccccagcc cgtcggtggt gagcgagaag gagaaggaag agttggagcg gctgcagaag
      901 gaagaggagg agaggaagaa gaggctgcag ctgtacgtgt tcgtgatgcg ctgcattgcc
      961 taccccttca acgccaagca gcccaccgac atggctcggc ggcagcagaa gatcagcaaa
     1021 cagcagctgc agactgtcaa ggaccgattc caggctttcc tcaatgggga gacccaaatc
     1081 gtggcagatg aagcctttat gaatgctgtc cagagctact atgaggtgtt tctcaagagt
     1141 gaccgtgtgg cccgcatggt acagagtggg ggctgctcag ccaacgactc tcgggaggtc
     1201 ttcaagaaac acatcgagaa gagggtacgc agcctacctg agattgatgg cctcagcaag
     1261 gagactgtgc taagctcctg gatggctaag tttgacgcca tctaccgtgg tgaggaggac
     1321 cccaggaagc agcaagcccg gatgacagcc agtgcagctt ctgagttgat tctgagcaag
     1381 gaacagctct atgagatgtt ccagaacatt cttgggatca agaaatttga acatcaactc
     1441 ctatataatg cgtgtcagct ggacaatcca gatgagcagg cagcccagat cagacgagag
     1501 ctggacggac gtctccaaat ggcagaccaa atagccaggg aacgcaaatt tcctaagttc
     1561 gtatccaaag aaatggaaaa tatgtacatc gaggagctga agtcctccgt caacctactc
     1621 atggccaact tggagagcat gccggtgtcc aagggtggag agttcaagct gcagaaactg
     1681 aaacgcagtc acaacgcgtc catcattgac atgggtgagg agagcgagaa ccagctgtcc
     1741 aagtcggatg tcctgctgtc cttctccttg gaggttgtca tcatggaagt ccaaggcctt
     1801 aagtccttgg ctccaaaccg aatcgtgtac tgcacaatgg aggtggaggg tggagagaaa
     1861 cttcagactg atcaagcaga ggcttctaaa ccaacctggg gcactcaagg cgacttctct
     1921 acaacccacg cgctgccagc tgttaaagta aagctattca cagagagcac tggtgtcctg
     1981 gccttggaag acaaggaact cgggcgggtt atcctccatc ccaccccaaa cagccccaaa
     2041 caatccgagt ggcacaaaat gacagtctcc aaaaactgcc ccgatcaaga cctcaaaatc
     2101 aaactcgctg tccgaatgga taagcctcaa aacatgaagc actctgggta cttatggacc
     2161 attggtaaga atgtgtggaa gaggtggaag aaaaggttct ttgtgttggt gcaggtcagc
     2221 cagtacactt ttgccatgtg cagctatcga gaaaagaaag cagaacctca ggaactccta
     2281 cagctggatg gctacaccgt ggattacacc gaccctcagc caggtttgga gggtggccga
     2341 gccttcttca atgcagtcaa agaaggagac acggtgatat ttgccagtga cgatgagcaa
     2401 gatcgaatcc tgtgggtcca agctatgtac agggccaccg ggcagtcgca caaacctgtg
     2461 ccccctaccc aggtgcagaa gctcaacgcc aagggcggaa acgtgcctca gctggatgcc
     2521 cccatctccc agttttacgc agatagagct caaaagcatg gcatggatga atttatctcg
     2581 tccaacccct gtaactttga ccacgcttcc ctctttgaga tggtacagcg gctgactttg
     2641 gatcacagac taaacgattc ctattcttgc ctgggctggt tcagtcctgg ccaggtgttt
     2701 gtgttagacg agtactgcgc tcggaatgga gtccgaggat gtcatcggca tctctgttac
     2761 cttagagact tgctggaacg ggcagaaaat ggcgccatga tcgaccccac gcttcttcat
     2821 tacagctttg ccttctgcgc atcccatgtc cacgggaaca ggcctgatgg aatcggaact
     2881 gtgactgttg aagaaaaaga gcgctttgaa gaaatcaaag agagactccg agttctgctg
     2941 gaaaatcaaa ttacacattt caggtactgc tttccgtttg gtcgacctga aggtgctttg
     3001 aaagcgactc tctcactttt ggaacgggtt ttgatgaaag atattgtcac tccagtaccc
     3061 caagaggagg tcaaaactgt catccgtaaa tgcctagagc aggctgctct agtcaactac
     3121 tctcgcctat ctgagtatgc caaaatcgaa gaaaatgtag gccgcttaat cactcccgcc
     3181 aaaaagctcg aagacacaat ccgtcttgct gaactagtca ttgaagtgct gcaacaaaac
     3241 gaagagcacc acgccgaggc ctttgcgtgg tggtcagact taatggtgga gcacgcggag
     3301 acgttcctgt cactctttgc agtggacatg gatgcagcat tagaggtgca acccccagac
     3361 acgtgggaca gtttcccact cttccagctg ctgaatgact ttctccttac agactataac
     3421 ttgtgcaatg gaaagtttca caaacacctg caggatctgt tcgccccact cgttgttaga
     3481 tacgtggatc tgatggagtc ctcaattgca caatctatcc acaggggctt tgagcgggag
     3541 tcatgggaac cagtcaataa cggatcgggt acatcagaag atttgttctg gaaacttgat
     3601 gcccttcaga ctttcatccg tgacctacac tggcctgagg aagagtttgg aaagcactta
     3661 gagcagcggc taaaactaat ggcaagcgac atgattgagt cctgcgtcaa gagaaccagg
     3721 attgcatttg aagttaagct gcaaaaaacc agtcgatcaa cagattttcg agtcccacag
     3781 tcaatatgca ccatgtttaa tgttatggtt gatgccaaag ctcaatcaac aaaactttgc
     3841 agcatggaaa tgggccagga gcatcaatac cattcaaaaa tagacgaact aattgaagaa
     3901 accgttaagg aaatgatcac actcttggtg gcaaagtttg taactatatt ggaaggcgta
     3961 ctggcaaagt tatccagata cgacgagggg actttgtttt cttcgtttct gtcatttacg
     4021 gtgaaggcgg catctaagta tgtggacgta cctaaacccg ggatggatgt ggccgacgcc
     4081 tacgtgactt tcgtccgcca ctctcaggat gtccttcgtg ataaggtcaa tgaggagatg
     4141 tatatagaaa ggttatttga tcaatggtac aacagctcca tgaacgtcat ctgcacctgg
     4201 ctgaccgacc ggatggacct ccagcttcac atttatcagc tgaaaacact aattaggatg
     4261 gtaaagaaaa cctacagaga tttccgattg caaggggtcc tggattccac cttaaacagc
     4321 aagacctatg agaccattcg aaatcggctc actgtagagg aagctaccgc gtccgtgagt
     4381 gagggagggg gcttgcaagg catcagcatg aaggacagcg atgaagagga cgaggaggat
     4441 gacggtggag gaggttctgg ccaccacact ggactagtgg atccggacta caaggatgac
     4501 gacgataagg ggaaatcata cccaacagtg tccgcagact accaggatgc cgtggagaaa
     4561 gccaagaaga agctgagagg gtttatcgca gaaaagaggt gcgcacctct gatgctgaga
     4621 ctggctttcc acagcgcagg cacctttgac aagggaacca aaacaggcgg accctttgga
     4681 acaatcaagc accctgctga actggcacat agtgccaaca atgggctgga catcgccgtg
     4741 cggctgctgg aacctctgaa agcagagttc ccaattctgt cctacgccga tttttatcag
     4801 ctggcaggag tggtcgctgt ggaggtcact gggggcccca aggtgccttt ccacccagga
     4861 cgggaggaca aaccagaacc acctccagag gggcgcctgc cagatccgac aaagggctcc
     4921 gaccatctgc gagatgtgtt tgggaaagct atgggcctga ctgaccagga tatcgtcgca
     4981 ctgtctggag ggcacaccat tggcgccgct cataaggaaa ggtcaggctt cgagggaccc
     5041 tggacaagca accccctgat tttcgacaat tcttacttta ctgaactgct gagtggagag
     5101 aaggaagggc tgctgcagct gcccagcgac aaagccctgc tgtccgatcc cgtgttcaga
     5161 cctctggtcg ataagtatgc agccgacgag gatgcttttt tcgcagatta cgcagaagca
     5221 catcagaagc tgtcagaact gggatttgcc gacgcctaag cggccgcgac tctagatcat
     5281 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5341 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5461 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     5521 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5581 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     5641 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5701 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5761 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5821 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5881 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5941 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6001 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6061 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     6121 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6181 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6301 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6361 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6421 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     6481 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6541 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6601 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6661 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6721 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6781 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6841 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6901 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6961 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     7021 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7081 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7141 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7201 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7261 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7321 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7381 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7441 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     7501 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     7561 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     7621 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     7681 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     7741 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7801 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7861 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7921 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7981 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8041 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8101 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8161 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8221 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8281 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8341 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8401 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8461 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8521 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8581 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     8641 atatggagtt ccg
//
