LOCUS       sequence-488453-        2649 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  atpA promoter + 5'UTR Chlamydomonas reinhardtii 2-3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2-3a_004_atpA_Promoter+5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      593..650
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(674..691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(845..1433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(925..944)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1616..1710)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1731..2390)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2305..2324
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2552..2570
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2574..2617)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2620..2641
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga gcaaaagtca tttttataac tggatctcaa aatacctata aacccattgt
       61 tcttctcttt tagctctaag aacaatcaat ttataaatat atttattatt atgctataat
      121 ataaatacta tataaataca tttacctttt tataaataca tttacctttt ttttaatttg
      181 catgatttta atgcttatgc tatctttttt atttagtcca taaaaccttt aaaggacctt
      241 ttcttatggg atatttatat tttcctaaca aagcaatcgg cgtcataaac tttagttgct
      301 tacgacgcct gtggacgtcc cccccttccc cttacgggca agtaaactta gggattttaa
      361 tgcaataaat aaatttgtcc tcttcgggca aatgaatttt agtatttaaa tatgacaagg
      421 gtgaaccatt acttttgtta acaagtgatc ttaccactca ctatttttgt tgaattttaa
      481 acttatttaa aattctcgag aaagatttta aaaataaact tttttaatct tttatttatt
      541 ttttcttttt tccattgaga ccactcatcg cgactagtag cggccgctgc agtccggcaa
      601 aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
      661 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1261 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1561 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct
     1621 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1681 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1741 ccctgccact cgtcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1801 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1861 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1921 tcaaaactgg tgaaactcac ccagggattg gctgatacga aaaacatatt ctcaataaac
     1981 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2041 agaaactgcc ggaaatcgtc gtggtattca ctccagagtg atgaaaacgt ttcagtttgc
     2101 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     2161 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     2221 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     2281 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     2341 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     2401 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     2461 tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga
     2521 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     2581 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
     2641 gcgatgagt
//
