LOCUS       sequence-488454-        2291 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  rbcL 5'UTR full Nicotiana tabacum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_032_rbcL_5'UTR_full_Nt
FEATURES             Location/Qualifiers
     source          1..2291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      235..292
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(316..333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(487..1075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(567..586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1258..1352)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1373..2032)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1947..1966
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2194..2212
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2216..2259)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2262..2283
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac tatgtatttg gcaaatcaaa taccatggtc taataatcaa acattctgat
       61 tagttgataa tattagtatt agttggaaat tttgtgaaag attcctgtga aaagtttcat
      121 taacacggaa ttcgtgtcga gtagaccttg ttgttgtgag aattcttaat tcatgagttg
      181 tagggaggga tttaatgtga gaccactcat cgcgactagt agcggccgct gcagtccggc
      241 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      301 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      481 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      661 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      901 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1021 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1081 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1141 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1201 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     1261 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1321 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1381 cgccctgcca ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1441 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1501 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1561 aatcaaaact ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa
     1621 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1681 gtagaaactg ccggaaatcg tcgtggtatt cactccagag tgatgaaaac gtttcagttt
     1741 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1801 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1861 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1921 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1981 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2041 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2101 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
     2161 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     2221 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta
     2281 gagcgatgag t
//
