LOCUS       sequence-488459-        2805 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  mScarlet-I C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5a_007_mScarlet-I
FEATURES             Location/Qualifiers
     source          1..2805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      749..806
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(830..847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1001..1589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1081..1100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1772..1866)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1887..2546)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2461..2480
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2708..2726
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2730..2773)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2776..2797
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct ttagtttcaa aaggtgaagc agtaattaaa gaatttatgc gtttcaaagt
       61 tcatatggaa ggttctatga atggtcacga atttgaaatt gaaggtgaag gtgaaggtcg
      121 tccatatgaa ggtacacaaa ctgctaaatt aaaagtaaca aaaggtggtc cattaccttt
      181 tagttgggat attttatctc cacaatttat gtatggttca cgtgcattta ttaaacaccc
      241 agcagacatt ccagattact ataaacaatc attcccagaa ggtttcaaat gggaacgtgt
      301 tatgaacttt gaagatggtg gtgcagttac tgtaacacaa gatacatcat tagaagatgg
      361 tactttaatt tataaagtta aattacgtgg tacaaatttt ccacctgatg gtcctgtaat
      421 gcaaaaaaaa acaatgggtt gggaagcttc aactgaacgt ttatatcctg aagatggtgt
      481 attaaaaggt gatattaaaa tggcattacg tttaaaagat ggtggtcgtt atttagctga
      541 ctttaaaaca acttataaag caaaaaaacc tgttcaaatg cctggtgctt ataacgttga
      601 tcgtaaatta gacattactt cacacaatga agattataca gtagttgaac aatatgaacg
      661 tagtgaaggt cgtcattcta caggtggtat ggatgaatta tataaagggt atgagaccac
      721 tcatcgcgac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
      781 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
      841 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      901 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      961 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1021 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1081 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1141 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1201 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1261 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1321 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1381 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1441 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1501 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1561 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1621 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1681 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1741 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     1801 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1861 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcgtc gcagtactgt
     1921 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1981 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     2041 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     2101 ggattggctg atacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     2161 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     2221 tattcactcc agagtgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     2281 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     2341 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     2401 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     2461 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     2521 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     2581 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     2641 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2701 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     2761 taaggatgat ttctggaatt cgcggccgct tctagagcga tgagt
//
