LOCUS       sequence-488464-        2633 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  psaB 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_013_psaB_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      577..634
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(658..675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(829..1417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(909..928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1600..1694)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1715..2374)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2289..2308
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2536..2554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2558..2601)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2604..2625
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac tcaaatgtta ctcgtggatg gctttggtat ttaggcgtag catgcagtaa
       61 atatatggcc aatggctcct cttttagcaa gttctcaact taaaatgcaa tctcttaacg
      121 agaatctaaa gctcagaaat atatttgaat taaaatttcc cacaggatta tggcgtagtc
      181 ataatatcaa ctaaaaaatc tttttaaatt ttaaaattta cttttttacg cttttgtatg
      241 caaagtttgc tttgcacctg aatagtttta ttaaattttt atttaatggt agtttaatag
      301 tagtaattta cttcaattaa acaaaaaaaa tcctaattgt ttatcccttt aaaagagcgc
      361 ttaaagtttt tttacttagt gaagtaaaaa taccgctccc ttctggtatt ttttcttttg
      421 atttaacaat tagcatttta accttttact tttctctcag tgttatactg cttaaaagtt
      481 tttaggtcat tagataatat ttaataatat tacatatagg gagtaagaca attttaatgt
      541 gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
      601 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1261 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1501 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1561 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1621 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1681 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcgtcgc
     1741 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1801 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1861 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1921 tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1981 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2041 cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2101 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2161 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2221 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2281 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2341 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2401 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2461 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     2521 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2581 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg agt
//
