LOCUS       sequence-488466-        3246 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  3' Homology psbH in the M2 position used for genome integration at 
            the psbH locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_005_psbH_3'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..3246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1190..1247
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1271..1288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1442..2030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1522..1541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2213..2307)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2328..2987)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2902..2921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3149..3167
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3171..3214)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3217..3238
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgcgc ttgccactgc cgaatataaa tatggttgag ttgcttagtt taccttagcg
       61 aaaagaagac ttagcagcta gccttaacaa acagttttat attttatgtt tgtgttaaat
      121 aaaattaaga aactttagct aaagtttccc aactcataga aacgtcatct aaaattaaag
      181 aactgttgta aatttctaaa atgattaata agaatgctgc aaataaaagg ataaatacag
      241 ccattaaaac agttgtaccc cagcctggta atactttacc tgcttctgag ttaagtggac
      301 gtaataaagt acctaatggt gtaactaaac caggttcttg gaagtctgaa tttacttttg
      361 atggtttagc tttagaagtt cctgttgcca taattgatta aatgaattaa gcgttattag
      421 cgctatttta tttactttct gtaaaaaata aggaaaatat tcttcagtgc attccctctc
      481 aggattataa atactctgag gataacgttc tctcgtcaag gggttgcttc ttgtgagtat
      541 agaaacctac tagcacaaga aataaattgc ataaaaatgt atttacctag gaccgcagta
      601 ggcagtccct tttccccttc agaactgcct gctttaaaag aatgaaaaaa ctgccttgtc
      661 tggtaagtaa aactctttaa ttactcacta aagacgatct agaagttctt tgttcatttt
      721 ttatttaata taatatttgt tatataaaaa ttaaataatt tttaattaat gtttaacttt
      781 gtaaggacag tttcaaagtg acatgaatgg ctactgcaaa aacgaagtaa gttattcttt
      841 ctcagggcaa aattttgagt agattaattt tgtttaaaaa tgtgggacac agtcgtcaag
      901 tcttttgaac tatctaagag atatgttgaa aagagaataa ttttattatt aaatgagcta
      961 tggaaagtcc agcttttttc tttacctttt ttttatggtt tcttctgtta agtgtaactg
     1021 gctattcagt ttatgttagt tttggtccac cttcaaaaaa attacgtgat ccttttgaag
     1081 aacacgaaga ttaaacaagt taaaaagtac tatttttaca agtgacttcg gtgcctcaga
     1141 cggagacgag actgagacca ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa
     1201 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     1261 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1321 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1441 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1861 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2101 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2161 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     2221 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     2281 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     2341 tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2401 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2461 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2521 aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct
     2581 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2641 aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc agtttgctca
     2701 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2761 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2821 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2881 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2941 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     3001 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     3061 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
     3121 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     3181 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagcg
     3241 atgagt
//
