LOCUS       sequence-488468-        2883 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  rpoB2 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_030_rpoB2_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      827..884
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(908..925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1079..1667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1159..1178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1850..1944)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1965..2624)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2539..2558
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2786..2804
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2808..2851)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2854..2875
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct taattctatg cataaatgaa attattttat tattttttta atcactttat
       61 gaatctggtt ctttcatttt ttagaatctt gttagcaaat ttctctacca gaatgggatg
      121 gcattggcag ttggaaaacc cggtacaagt caatacattc tttagttttt tgcatattcg
      181 aaaggacgtc cccttacggg acgtcccttt tgggacgcca gtggacgtca gtggcagtgg
      241 taccgccact gcctccttcc ccttcccctt cgggacgtcc ccttacggga atataaatat
      301 tagtggcagt tgcctccttc cctttcccct tcgggacgtc cccttacggg aatataaata
      361 ttagtggcag ttgcctcctt ccctttcccc ttcgggacgt ccccttacgg gaatataaat
      421 attagtggca gttgcctcct tccctttccc cttcgggacg tccccttacg ggaatataaa
      481 tattagtggc agttgcctcc ttccccttcg ggcaagtaaa cttaggagta tataaatata
      541 ggatgttaat actgcggagc aggcagtggc ggtaccactg ccactaaaat ttatttgccc
      601 gaaggggacg tcctgccaac tgcctaggca gccaataaat tttatcgcag tatatgtata
      661 tgcttcccct tctgggatag aagtaaacat ctccgaaggc acgttcttta cggtctatat
      721 attcttgcag tggcttgcga ctgctactaa tgtaatgttc ccaaggggtt taatcccgat
      781 ttaagcgctt gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg
      841 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
      901 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1081 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1801 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
     1861 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     1921 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     1981 cactcgtcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     2041 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     2101 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     2161 ctggtgaaac tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta
     2221 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2281 tgccggaaat cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg
     2341 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     2401 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     2461 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     2521 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     2581 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     2641 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     2701 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
     2761 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     2821 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg
     2881 agt
//
