LOCUS       sequence-488473-        8515 bp    DNA     circular SYN 06-OCT-2025
DEFINITION  For simultaneous monitoring of both transcription and translation of
            genes in plant tissues: C-terminus fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Banda J, Ghosh S, Roy D, Ingole KD, Clark L, Sharma E, Kakkunath S, 
            Sue-Ob K, Bhosale R, Band L, Ghosh S, Wells D, Atkinson J, Provart 
            NJ, Bennett MJ, Lilley KS, Jones A, De Lucas M, Bishopp A, 
            Sadanandom A
  TITLE     Elucidating tissue and subcellular specificity of the entire SUMO 
            network reveals how stress responses are fine-tuned in a eukaryote.
  JOURNAL   Sci. Adv., 11, eadw9153 (2025)
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci. Adv., 
            11, eadw9153 (2025)"
COMMENT     Sequence Label: pMDS1
FEATURES             Location/Qualifiers
     source          1..8515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        85..430
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     373..393
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1337..2047
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELYK"
     primer_bind     complement(1482..1500)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1750..1769
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2066..2318
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2416..2739)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     2421..2440
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2634..2656
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2648..2665
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2649..2665
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2666..2722
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2735..2751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2735..2751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2748..2770)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2759..2775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2783..2789,2790..2807,2808..2813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2828..2849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2966..2983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(3062..3064,3065..3775)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             3797..3886
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             join(3965..3967,3968..4675)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     polyA_signal    4985..5161
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(5225..5245)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5225..5243)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    5322..5346
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5437..6025)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5517..5536)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6116..6904)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7956..8391
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 cttggcagga tatattgtgg tgtaacgttg gcgcgcctac tccaaaaatg tcaaagatac
       61 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt cgggaaacct
      121 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      181 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atgcctctgc
      241 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      301 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
      361 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      421 ggagaggaca gcccagatca ctagtcttaa gtccggagct agctctagaa tggcaaagga
      481 tgtggaagcc gttcccggag aaggatttca gacaagagac tatcaagatc cgccaccagc
      541 tccgtttatt gatggagcgg agctaaagaa gtggtctttc tacagagcag ttatcgcaga
      601 gttcgtagcc actctcctct tcttatacat caccgttttg acagtcatcg gttacaagat
      661 tcagtccgat actgatgccg gtggcgtaga ttgcggcgga gttggaatcc tcggtatcgc
      721 ttgggccttt ggtggtatga tcttcatcct cgtctactgc accgccggta tctctggtgg
      781 tcacattaac ccagcggtga catttgggct attcttggca cgtaaagtgt cgttacctag
      841 ggccctattg tacataatcg ctcagtgttt gggtgcgatt tgtggagttg gttttgtcaa
      901 agccttccaa agctcttact acacccgtta cggaggtgga gccaactctc tagccgatgg
      961 ctacagcaca gggaccggtc tagccgcaga gatcattggt actttcgttc ttgtctacac
     1021 cgtcttctct gccactgacc ccaaacgtag tgccagagac tcccacgttc cggtgttggc
     1081 gccacttcca atcggatttg ccgtgttcat ggtacatttg gctaccattc ccattaccgg
     1141 aaccggaatt aacccggcaa ggagtttcgg agctgccgta atctacaaca agagcaagcc
     1201 atgggatgac cactggatat tttgggttgg accattcatt ggagctgcga tagctgcatt
     1261 ctaccaccaa ttcgttctaa gagcttcagg ttctaagtct cttggatcat tcagaagtgc
     1321 tgccaacgtc ggatccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     1381 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     1441 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1501 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     1561 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     1621 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1681 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1741 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1801 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1861 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1921 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     1981 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg aggagctgta
     2041 caagtaaagc ggccgcccgg ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag
     2101 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     2161 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     2221 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     2281 taaattatcg cgcgcggtgt catctatgtt actagatcgg ggcgcgccct cgagagtaga
     2341 agagccggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     2401 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     2461 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     2521 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2581 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2641 acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat cctctagagt
     2701 cgacctgcag gcatgcaggc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2761 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     2821 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2881 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2941 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3001 tgcggcgagc ggtatcgctc ttcatggatc cggaggtggt ggttctggtg ggggtggctc
     3061 tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     3121 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     3181 caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     3241 cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
     3301 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     3361 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     3421 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     3481 gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg
     3541 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga
     3601 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     3661 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     3721 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagcatga
     3781 tggcattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     3841 cccggactat gcaggatcct atccatatga cgttccagat tacgcttccg gctcgggcca
     3901 gctgttgaat tttgaccttc ttaagcttgc gggagacgtc gagtccaacc ctgggccccc
     3961 cggggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4021 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4081 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     4141 cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     4201 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4261 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4321 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4381 caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca agcagaagaa
     4441 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     4501 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4561 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     4621 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacattgc
     4681 tgcagcggcc gaattcaagc gtgaggagca agcaaggaaa gctaaggtga acaatgagaa
     4741 aaagacggaa atagtgaaac cagagagttg tagcaatgaa ggagatgtca aggatctgaa
     4801 aagaaaggac tctgaggatg gaaacgaggg tgaggaagaa gaagcttctt cgaaaccgaa
     4861 aaagccaaaa gttgctcttt ctcatcttca ggacattgac gacacagaag ctgatcaaga
     4921 agaagagtaa ggtgtcggta cgctgaaatc accagtctct ctctacaaat ctatctctct
     4981 ctattttctc cataaataat gtgtgagtag tttcccgata agggaaatta gggttcttat
     5041 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     5101 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     5161 cgatgggcta gagcggacta gttctagagc ggccgccacc gcggtggagc tccagctttt
     5221 gttcccttta gtgagggtta attgcgcgct agctcactca aaggcggtaa tacggttatc
     5281 cacagaatca ggggataacg caggaaagaa catgaaggcc ttgacaggat atattggcgg
     5341 gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa
     5401 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5461 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5521 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5581 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5641 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5701 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5761 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5821 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5881 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc
     5941 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6001 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6061 tcagtggaac gaaaactcac gttaagggat tttggtcatg aattcttgtt agacattatt
     6121 tgccgactac cttggtgatc tcgcctttca cgtagtgaac aaattcttcc aactgatctg
     6181 cgcgcgaggc caagcgatct tcttgtccaa gataagcctg cctagcttca agtatgacgg
     6241 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     6301 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     6361 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     6421 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     6481 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     6541 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     6601 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     6661 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     6721 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca
     6781 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     6841 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     6901 tcatgatgtt taactcctga attaagccgc gccgcgaagc ggtgtcggct tgaatgaatt
     6961 gttaggcgtc atcctgtgct cccgagaacc agtaccagta catcgctgtt tcgttcgaga
     7021 cttgaggtct agttttatac gtgaacaggt caatgccgcc gagagtaaag ccacattttg
     7081 cgtacaaatt gcaggcaggt acattgttcg tttgtgtctc taatcgtatg ccaaggagct
     7141 gtctgcttag tgcccacttt ttcgcaaatt cgatgagact gtgcgcgact cctttgcctc
     7201 ggtgcgtgtg cgacacaaca atgtgttcga tagaggctag atcgttccat gttgagttga
     7261 gttcaatctt cccgacaagc tcttggtcga tgaatgtgcc atagcaagca gagtcttcat
     7321 cagagtcatc atccgagatg taatccttcc ggtaggggct cacacttctg gtagatagtt
     7381 caaagccttg gtcggatagg tgcacatcga acacttcacg aacaatgaaa tggttctcag
     7441 catcaaatgt ttccgccacc tgctcaggga tcaccgaaat cttcatatga cgcctaacgc
     7501 ctggcacagc ggatcgcaaa cctggcgcgg cttttggcac aaaaggcgtg acaggtttgc
     7561 gaatccgttg ctgccacttg tttaatagac tggatggagg cggataaagt tgcaggacca
     7621 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7681 cgtggatctc gcggtatcat tgcagcactg ccatgatgat atatttttat cttgtgcaat
     7741 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
     7801 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
     7861 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
     7921 tttctggctg gatgatgggg cgattcagga gatccccatc caacagcccg ccgtcgagcg
     7981 ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga aaccgctaat
     8041 gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta tccacgtgaa
     8101 atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct aataaggtca
     8161 cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca gatcaacagc
     8221 ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agtatgttca attagctttt
     8281 caattatgaa tatatatatc aattattggt cgcccttggc ttgtggacaa tgcgctacgc
     8341 gcaccggctc cgcccgtgga caaccgcaag cggttgccca ccgtcgagcg cctttgccca
     8401 caacccggcg gccggccgca acagatcgtt ttataaattt ttttttttga aaaagaaaaa
     8461 gcccgaaagg cggcaacctc tcgggcttct ggatttccga tccccggaat tagat
//
